A monoclonal antibody, WCC4, recognizes a developmentally regulated ganglioside containing α-galactose and α-fucose present in the rat nervous system

Suchy, Sharon F.; Yamamoto, Miyuki; Barbero, Linda; Schwarting, Gerald A.

doi:10.1016/0006-8993(88)91155-9

Cited by 24 publications

(4 citation statements)

References 32 publications

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“…With the exception of the receptors for glutamatergic synapses described above, relatively few specific markers for the OML have been reported. However, while most, if not all, neurons in the forebrain contain the GMl ganglioside recognized by MAb WCC4, this antigen is enriched in the neuropil of the dentate OML and the stratum lacunosum-moleculare of hippocampus (Suchy et al, 1988). We have characterized a number of Mabs that appear to be unique markers of the OML (Woodhams et al, 1992b).…”

Section: Markers For Neuropil-discontinuously Expressedmentioning

confidence: 99%

Laminar and region‐specific cell surface markers in the entorhinal cortex and hippocampus

Woodhams¹

1993

Hippocampus

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Section: Markers For Neuropil-discontinuously Expressedmentioning

confidence: 99%

Laminar and region‐specific cell surface markers in the entorhinal cortex and hippocampus

Woodhams¹

1993

Hippocampus

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“…Differential expression of cell surface carbohydrates has been used extensively to distinguish among subsets of neurons in the developing and mature nervous system ( Sieber- Blum and Cohen, 1978;Grunwald et al, 1985;Streit et al, 1985;Yamamoto et al, 1985;Dodd and Jessell, 1986; Mori, 1986;Nakagawa et al, 1986;Regan et al, 1986;Bastiani et al, 1987;Blum and Barnstable, 1987;Schwarting et al, 1987;Doddet al, 1988; Mendez-* To whom correspondence should be addressed. Otero et al, 1988;Suchy et al, 1988;Alvarez et al, 1989;Ashton et al, 1990;Scott et al, 1990;Stainier et al, 1991;Schwarting et al, 1992). This approach has led to the identification of several developmentally regulated epitopes that are believed to mediate cellular interactions important for neural development, including neuronal migration, axon outgrowth and fasciculation, and recognition (reviewed by Yamamoto and Schwarting, 1992).…”

Section: Introductionmentioning

confidence: 99%

Lectin binding distinguishes between neuroendocrine and neuronal derivatives of the sympathoadrenal neural crest

1995

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Lectin cytochemistry was used to identify surface epitopes selectively expressed by chromaffin cell chemoreceptors (glomus cells) in the rat carotid body. Unexpectedly, these studies revealed that binding sites for peanut agglutinin (PNA; Arachis hypogea) were highly expressed by all neuroendocrine-derivatives of the sympathoadrenal neural crest, including glomus cells, small, intensely fluorescent cells, and adrenal chromaffin cells in situ. In contrast, principal sympathetic neurons did not express PNA receptors. PNA binding was inhibited by 2% galactose. To determine whether expression of PNA receptors was selectively induced by neuroendocrine differentiation of sympathoadrenal precursors, we compared PNA labeling of embryonic sympathoblasts in the presence of either nerve growth factor (NGF) or the synthetic glucocorticoid dexamethasone (DEX). DEX-treated cells, which expressed several neuroendocrine traits, bound PNA, whereas NGF-treated neuronal derivatives did not. In addition, to examine whether expression of existing PNA receptors was down-regulated by neuronal differentiation of chromaffin cells, we compared labeling of PC12 cells, which normally bind PNA, in the presence and absence of NGF. Although PC12 cells acquired characteristic neuronal morphologies in the presence of NGF, they did not lose PNA labeling, even after 8 days of NGF treatment. These findings indicate that neuronal and neuroendocrine derivatives of the sympathoadrenal lineage can be distinguished by differential expression of carbohydrate epitopes and suggest that PNA receptors are induced by neuroendocrine differentiation.

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“…GD3 from buttermilk chromatographs as a double band because of heterogeneity in the lipid portion of the molecule. Glycolipids were extracted from adult mouse cerebellum as described (13). The acidic glycolipid fraction obtained from DEAE chromatography was analyzed by TLC and by TLC immunostaining as described (14).…”

mentioning

confidence: 99%

Compartmentation in mammalian cerebellum: Zebrin II and P-path antibodies define three classes of sagittally organized bands of Purkinje cells.

Leclerc

Schwarting

Herrup

et al. 1992

Proc. Natl. Acad. Sci. U.S.A.

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The e ve roles of genetic and epigenetc factors in generation of pattern formation in the vertebrate nervous system are still poorly eucidated. The m cerebellum is subdivided In p i modules defne by anatomical, physiological, and biochemical criteria. Immunostaining of adult mouse ce l with two monoclonal antbodies, P-path, which recognizes 9-0-acetylated glycoilpids, number of bands is preserved during phylogeny (4, 5). In mammalian cerebellum, ZII antibody stains on each side of the midline, three bands in the vermis, one at the margin of the vermis and the hemisphere, and three in the hemisphere, separated by interbands of ZII-negative Purkinje cells. There is a close relationship between Zebrin compartments and the segregation of cerebellar afferent terminal fields (6, 7). An identical periodic pattern is also revealed by using several other markers that subdivide the cerebellar cortex into two sets of modules-one corresponding to Zebrin-positive compartments and one corresponding to Zebrin-negative compartments (5, 8, 9). Immunostaining of adult mouse cerebellum with a monoclonal antibody, P-path, that recognizes

show abstract

A monoclonal antibody, WCC4, recognizes a developmentally regulated ganglioside containing α-galactose and α-fucose present in the rat nervous system

Cited by 24 publications

References 32 publications

Laminar and region‐specific cell surface markers in the entorhinal cortex and hippocampus

Laminar and region‐specific cell surface markers in the entorhinal cortex and hippocampus

Lectin binding distinguishes between neuroendocrine and neuronal derivatives of the sympathoadrenal neural crest

Compartmentation in mammalian cerebellum: Zebrin II and P-path antibodies define three classes of sagittally organized bands of Purkinje cells.

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