A monoclonal antibody to aflatoxin B<sub>1</sub>: detection of the mycotoxin by enzyme immunoassay

Candlish, A. A. G.; Stimson, W. H.; Smith, John E.

doi:10.1111/j.1472-765x.1985.tb01489.x

Cited by 47 publications

(14 citation statements)

References 14 publications

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“…Daly and collaborators developed a Biacore-based assay using a polyclonal antibody in PBS with a limit of detection of 3 ng mL 21 [10] and an scFv-based Biacore assay with a limit of detection of 3 ng mL 21 in PBS and 0.75 ng mL 21 in spiked grain. [17] The limits of detection described in this paper also compare favorably with several other immunoassay formats including a fluorescence polarization assay for aflatoxins with a range of detection between 5 and 200 ppb, [27] a dipstick assay with a limit of detection of 2 ng mL 21 , [28] and ELISA formats described by Candlish et al, [29] Aldao et al, [30] and Daly et al [10,17] with limits of detection at 0.2 ng mL 21 , 0.25 mg kg 21 , 3 ng mL 21 , and 98 ng mL 21 , respectively. Analytical techniques, including the HPLC detection system described by Kussak et al [31] have offered greater sensitivity with limits of detection for aflatoxins B 1 , B 2 , G 1 , and G 2 at 6.8 pg mL 21 in urine, a sol-particle lateral flow immunoassay with limits of detection of 0.1 ppb in buffer and 10 ppb in grain samples, [32] and an immunoaffinity fluorometric biosensor with a lower limit of detection, at 0.1 ppb.…”

Section: Spr-based Assay For Detection Of Afb 1 239mentioning

confidence: 79%

Surface Plasmon Resonance‐Based Immunoassay for the Detection of Aflatoxin B₁Using Single‐Chain Antibody Fragments

Dunne

Daly

Baxter³

et al. 2005

Spectroscopy Letters

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Aflatoxin B 1 (AFB 1 ) is a highly toxic secondary metabolite of the fungal species Aspergillus flavus and Aspergillus parasiticus produced under certain environmental conditions. The gene encoding an AFB 1 -specific single-chain fragment variable (scFv) was isolated from a pre-immunized phage display library and used to express a monomeric and dimeric scFv, specific for AFB 1 , in Escherichia coli. The monomeric and dimeric scFv were then applied to the development of surface plasmon resonancebased inhibition immunoassays for the detection of AFB 1 . Regeneration of the sensor surface, which consisted of a CM5 chip immobilized with an AFB 1 derivative, was investigated and enabled at least 75 binding regeneration cycles. The inhibition assays developed had ranges of detection between 390 and 12,000 pg mL 21 (ppb) for the monomeric scFv and between 190 and 24,000 pg mL 21 (ppb) for the dimeric scFv, with coefficients of variation for the inter-day variability studies ranging from 1.9 -4.18% and 3 -11.53%, respectively.

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Section: Spr-based Assay For Detection Of Afb 1 239mentioning

confidence: 79%

Surface Plasmon Resonance‐Based Immunoassay for the Detection of Aflatoxin B₁Using Single‐Chain Antibody Fragments

Dunne

Daly

Baxter³

et al. 2005

Spectroscopy Letters

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“…Previous reports have indicated that antibodies raised against AFB 1 are AFB 1 specific (12,17) and that antibodies raised against AFB 2 are AFB 2 specific (18). This is the normal immune response between a hapten and its corresponding antibody.…”

Section: Resultsmentioning

confidence: 98%

Development of a Novel Immunoaffinity Column for Aflatoxin Analysis Using an Organic Solvent-Tolerant Monoclonal Antibody

Uchigashima

Saigusa²,

Yanagawa³

et al. 2009

J. Agric. Food Chem.

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An organic solvent-tolerant monoclonal antibody specific to aflatoxins B(1), B(2), G(1), G(2), and M(1) (AFB(1), AFB(2), AFG(1), AFG(2), and AFM(1)) was prepared. In an indirect competitive enzyme-linked immunosorbent assay, the half maximal inhibitory concentration (IC(50)) values were 1.9, 2.1, 2.1, 2.4, and 2.8 ng/mL for AFB(1), AFB(2), AFG(1), AFG(2), and AFM(1), respectively. Antibody reactivity was retained at 40% methanol concentration or at acetonitrile concentrations up to 40%. An immunoaffinity column (IAC) was prepared using agarose gel beads with bound antibody. The IAC retained the tested AFs that were 89, 90, 95, 90, and 89% for AFB(1), AFB(2), AFG(1), AFG(2), and AFM(1) at 20% acetonitrile concentrations or that were 81, 87, 79, and 83% for AFB(1), AFB(2), AFG(1), and AFG(2) at 60% methanol concentrations. Roasted peanuts and seven kinds of spices were spiked with 8.0, 1.0, 6.0, and 1.0 ng for AFB(1), AFB(2), AFG(1), and AFG(2) per 1 g sample and extracted with 90% acetonitrile. The roasted peanuts and cayenne pepper out of the spices were also extracted with 70% methanol. The extracts were diluted 5-fold with phosphate-buffered saline and applied to the IAC. The spiked aflatoxins were recovered with satisfactory rates: 78 (RSD, 2.1%) to 127% (RSD, 1.7%). The developed IAC was used for the analysis of aflatoxins in naturally contaminated samples of roasted peanuts and cayenne pepper. The newly developed IAC showed substantially organic solvent tolerance at the concentration that could not be used for existing IACs, and the column showed good ability to clean up samples for food analysis.

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“…Aflatoxins, produced by Aspergillus species, are especially relevant to the dairy industry. Competitive immunoassays with monoclonal antibodies have been developed and are commercially available now for aflatoxin types Bl, B2, Ml, Gl, and G2 (192). These tests allow toxins to be detected with a sensitivity of 1 to 200 ng per g food.…”

Section: Determination Of Toxinsmentioning

confidence: 99%

Changing perspectives in food microbiology: Implementation of rapid microbiological analyses in modern food processing

Veld

Hartog

Hofstra

1988

Food Reviews International

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This review article summarizes the perspective for the introduction of novel microbiological techniques into modern food manufacturing and processing. Changes are discussed that have occurred recently in the microbiological control of food operations (process microbiology)-for example, the "hazard analysis critical control point" (HACCP) concept, which can be elaborated to a longitudinal integrated chain assessment by monitoring critical control points. In addition, we describe the recent developments in rapid analytical methodology that can be implemented under these concepts. These techniques are based on two major approaches; that is, they are either aimed at estimating the total microbial load as a hygiene parameter, or at detecting and enumerating specific pathogenic or toxigenic microorganisms and their metabolites. We conclude that, at this moment, several of these methods are significant improvements compared with the conventional techniques. However their use in preventive quality assurance by integrated chain assessment, as well as in routine food (end product) control, has been limited to certain areas of the food industries as yet.

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A monoclonal antibody to aflatoxin B₁: detection of the mycotoxin by enzyme immunoassay

Cited by 47 publications

References 14 publications

Surface Plasmon Resonance‐Based Immunoassay for the Detection of Aflatoxin B₁Using Single‐Chain Antibody Fragments

Surface Plasmon Resonance‐Based Immunoassay for the Detection of Aflatoxin B₁Using Single‐Chain Antibody Fragments

Development of a Novel Immunoaffinity Column for Aflatoxin Analysis Using an Organic Solvent-Tolerant Monoclonal Antibody

Changing perspectives in food microbiology: Implementation of rapid microbiological analyses in modern food processing

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A monoclonal antibody to aflatoxin B1: detection of the mycotoxin by enzyme immunoassay

Cited by 47 publications

References 14 publications

Surface Plasmon Resonance‐Based Immunoassay for the Detection of Aflatoxin B1Using Single‐Chain Antibody Fragments

Surface Plasmon Resonance‐Based Immunoassay for the Detection of Aflatoxin B1Using Single‐Chain Antibody Fragments

Development of a Novel Immunoaffinity Column for Aflatoxin Analysis Using an Organic Solvent-Tolerant Monoclonal Antibody

Changing perspectives in food microbiology: Implementation of rapid microbiological analyses in modern food processing

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A monoclonal antibody to aflatoxin B₁: detection of the mycotoxin by enzyme immunoassay

Surface Plasmon Resonance‐Based Immunoassay for the Detection of Aflatoxin B₁Using Single‐Chain Antibody Fragments

Surface Plasmon Resonance‐Based Immunoassay for the Detection of Aflatoxin B₁Using Single‐Chain Antibody Fragments