A monoclonal antibody that recognizes a neo-antigen exposed in the E domain of fibrin monomer complexed with fibrinogen or its derivatives: its application to the measurement of soluble fibrin in plasma

Soe, Gilbu; Kohno, Isao; Inuzuka, K; Itoh, Yoh; Matsuda, Michio

doi:10.1182/blood.v88.6.2109.bloodjournal8862109

Cited by 57 publications

(36 citation statements)

References 31 publications

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“…Concentration of fibrinogen in plasma is dominating (2-4 mg/mL), therefore, a high selectivity of fibrin-targeting ligands is required to avoid their complete scavenging by fibrinogen during in vivo circulation. To overcome this burden, several laboratories tried to develop monoclonal antibodies specific to fibrin but those recognize soluble fibrin and its D-dimer as well, or substantially cross-react with fibrinogen [8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Targeting Human Thrombus by Liposomes Modified with Anti-Fibrin Protein Binders

et al. 2019

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Development of tools for direct thrombus imaging represents a key step for diagnosis and treatment of stroke. Nanoliposomal carriers of contrast agents and thrombolytics can be functionalized to target blood thrombi by small protein binders with selectivity for fibrin domains uniquely formed on insoluble fibrin. We employed a highly complex combinatorial library derived from scaffold of 46 amino acid albumin-binding domain (ABD) of streptococcal protein G, and ribosome display, to identify variants recognizing fibrin cloth in human thrombus. We constructed a recombinant target as a stretch of three identical fibrin fragments of 16 amino acid peptide of the Bβ chain fused to TolA protein. Ribosome display selection followed by large-scale Enzyme-Linked ImmunoSorbent Assay (ELISA) screening provided four protein variants preferentially binding to insoluble form of human fibrin. The most specific binder variant D7 was further modified by C-terminal FLAG/His-Tag or double His-tag for the attachment onto the surface of nanoliposomes via metallochelating bond. D7-His-nanoliposomes were tested using in vitro flow model of coronary artery and their binding to fibrin fibers was demonstrated by confocal and electron microscopy. Thus, we present here the concept of fibrin-targeted binders as a platform for functionalization of nanoliposomes in the development of advanced imaging tools and future theranostics.

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Section: Introductionmentioning

confidence: 99%

Targeting Human Thrombus by Liposomes Modified with Anti-Fibrin Protein Binders

et al. 2019

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“…Fibrin clots formed with either thrombin or Reptilase were solubilized by 20 mmol/L acetic acid, and they were expressed as desAABB-fibrin monomer (desAABB-FM) and desAA-fibrin monomer (desAA-FM), respectively. Soluble Fibrin monomer-fibrinogen complex (SF) was prepared as described by Soe et al 6…”

Section: Fibrinogen-related Antigensmentioning

confidence: 99%

“…JIF-23, 7 a monoclonal antibody that recognizes the amino-terminal region of fragment D 1 , and IF-43, 6 a monoclonal antibody that recognizes a neo-antigen exposed in the E domain of FM complexed with fibrinogen or its derivatives and MIF-220, were our own products.…”

Section: Antibodiesmentioning

confidence: 99%

Clinical application of a new latex photometric immunoassay reagent, LPIA‐GENESIS D‐dimer, and its performance in patient‐derived plasma samples

Nagahama¹,

Nozaki²,

Sakurai³

et al. 2020

Int J Lab Hematology

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Introduction:We previously reported an antibody MIF-220 that recognizes a specific structure induced on the surface of thrombin-activated E-domain of one fibrin molecule bound with the D-domains of other fibrinogen/fibrin molecules. Utilizing MIF-220, we produced a test kit for cross-linked fibrin degradation products (XDP), LPIA-GENESIS D-dimer (LG-DD), and evaluated basic performance characteristics for clinical application. We then attempted to apply LG-DD to see its eligibility in clinical plasma samples. Method:The characteristic performances requested for clinical use were studied including limit of quantitation, within-run imprecision, day-to-day imprecision, antigen excess, interference study, and method comparison with LPIAACE-Ddimer (ACE-DD) available on the market. Results:The performance characteristics were all satisfactory. Extraordinarily high concentrations of XDP are occasionally obtained by ACE-DD in samples with collection problems, but not by LG-DD, indicating that a certain XDP species present in the former was not measured by LG-DD. Structural studies suggested that the "B-b" set of polymerization sites must be involved as well in the maintenance of cross-linked fibrin in vivo. Conclusion:LG-DD was able to measure a wide range of XDP, that is, 0.20-35.0 μg FEU/mL that covers the levels of XDP in most of the clinical samples.LG-DD was found to almost avoid false-positive results noticed in samples as mentioned above, and this feature seems to be preferable to established kits for the measurement of XDP. K E Y W O R D Santibody for D-dimer, blood collection, cross-linked fibrin degradation products (XDP), D-dimer, latex photometric immunoassay (LPIA)

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“…Plasma levels of SFMC, D-dimer, PAI-1, and platelet (Plt) count were measured before and on postoperative days 1, 3, and 7. SFMC levels were measured by a latex immunoagglutination assay (Mitsubishi Chemical Medience Corporation, Tokyo, Japan) using the monoclonal antibody IF-43 15) . D-dimer levels were measured by a latex immunoagglutination assay (Mitsubishi Chemical Medience Corporation) using the monoclonal antibody JIF-23 16) .…”

Section: Blood Coagulation-fibrinolysis Markersmentioning

confidence: 99%

D-dimer predicts pulmonary embolism after low-risk spine surgery

Inoue

Watanabe

Okami³

et al. 2018

Spine Surg Relat Res

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Introduction: Pulmonary embolism (PE) is a risk of mortality following spine surgery. Many studies have demonstrated that deep venous thrombosis (DVT) may affect and actually advance to PE, but few studies have shown how venous thromboembolism (VTE), including PE and DVT, affect blood markers after spine surgery. In this study, we examined changes in blood markers with PE or DVT after low-risk spine surgery, namely cervical laminoplasty or lumbar laminectomy. Methods: Seventy-two spine surgery patients were studied. A 16-row multidetector computed tomography was performed before and 3 d after the surgery. Patients with a history of cerebral vascular accident or arterial thrombotic episode or presurgical asymptomatic PE or DVT were excluded. Plasma levels of soluble fibrin monomer complex, D-dimer, plasminogen activator inhibitor type-1 (PAI-1), and white blood cell and platelet counts were measured preoperatively and postoperatively at days 1, 3, and 7. Results: No patient developed symptomatic post-surgical VTE. Six patients with asymptomatic PE and six with asymptomatic DVT were detected post-surgery, including one patient with both. D-dimer postoperatively at days 3 and 7 was significantly higher in the post-op PE group than in the no-PE group. PAI-1 preoperatively was significantly higher in the DVT and VTE groups than in the no-DVT and no-VTE groups. Conclusions: Elevated D-dimer at postoperative days 3 and 7 is a predictive factor for the early diagnosis of PE after spine surgery. Moreover, elevated PAI-1 preoperatively is a predictive factor for the early diagnosis of DVT and VTE. Consequently, PE may occur through a pathway other than DVT.

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A monoclonal antibody that recognizes a neo-antigen exposed in the E domain of fibrin monomer complexed with fibrinogen or its derivatives: its application to the measurement of soluble fibrin in plasma

Cited by 57 publications

References 31 publications

Targeting Human Thrombus by Liposomes Modified with Anti-Fibrin Protein Binders

Targeting Human Thrombus by Liposomes Modified with Anti-Fibrin Protein Binders

Clinical application of a new latex photometric immunoassay reagent, LPIA‐GENESIS D‐dimer, and its performance in patient‐derived plasma samples

D-dimer predicts pulmonary embolism after low-risk spine surgery

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