A monoclonal antibody targeting the dimer interface of epidermal growth factor receptor (EGFR)

Guo, Tujing; Zhao, Lin; Zhang, Yawen; Liu, Guoqiang; Yao, Yuanhang; Li, Huangjin

doi:10.1016/j.imlet.2016.10.011

Cited by 8 publications

(4 citation statements)

References 20 publications

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“…About 70% of liver cancer cells express high levels of EGFR, whereas activated EGFR plays an important role in cell migration, which in turn lead to angiogenesis (21). Thus, inhibition of EGFR is a potential treatment of cancer (22,23). We found that HCV-E2 increased the expression of miR-490 in MC-derived exosomes and recipient HCC cells, thereby reducing the activity of EGFR/AKT/ERK1/2 pathway and inhibiting the migration of HCC cells.…”

Section: Discussionmentioning

confidence: 99%

HCV-E2 inhibits hepatocellular carcinoma metastasis by stimulating mast cells to secrete exosomal shuttle microRNAs

Zhen

et al. 2017

Oncology Letters

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Exosomal miRNAs are emerging as mediators of the interaction between mast cells (MCs) and tumor cells. The exosomal miRNAs can be internalized by liver cancer cells to inhibit cell metastasis. We explored the interaction between MCs and hepatocellular carcinoma (HCC) cells. We used hepatitis C virus E2 envelope glycoprotein (HCV-E2) to stimulate MCs and harvest MCs-derived exosomes to detect the miRNAs and changes of exosomal miRNAs before and after stimulation. Through miRNA microarray analysis, we identified 19 differentially expressed miRNAs in exosomes derived from MCs with or without HCV-E2 treatment. HCV-E2 not only increased the level of miRNA-490 in MCs and their secreted exosomes but also increased the levels of miRNA-490 in recipient HepG2 cells, which ultimately inhibited the ERK1/2 pathway. The transfection of antagomiR-490 significantly decreased the levels of miR-490 in MCs, MCs-derived exosomes, and recipient HepG2 cells and increased migration of HepG2, indicating that miR-490 is involved in the regulation of HepG2 cell migration. The present study suggests that MCs can inhibit HCC cell metastasis by inhibiting the ERK1/2 pathway by transferring the exosomal shuttle microRNAs into HCC cells, which provides new insights for the biological therapy of HCC induced by hepatitis C.

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Section: Discussionmentioning

confidence: 99%

HCV-E2 inhibits hepatocellular carcinoma metastasis by stimulating mast cells to secrete exosomal shuttle microRNAs

Zhen

et al. 2017

Oncology Letters

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“…EGFR is a 170-kDa protein consisting of 3 domains: an extracellular domain that binds ligands, a transmembrane domain, and an intracellular domain with tyrosine kinase activity [ 24 ]. EGF binds with EGFR and induces tyrosine kinase phosphorylation of its intracellular domain [ 25 ], which then recruits effector proteins to its phosphorylated C-terminal domain and activates effector protein-mediated signal pathways that regulate proliferation, survival, differentiation, migration, invasion, and injury repair of cells [ 26 ]. EGFR is expressed in many malignant tumor cell types [ 27 – 29 ].…”

Section: Discussionmentioning

confidence: 99%

Cyclic Guanosine Monophosphate (cGMP)-Dependent Protein Kinase II Blocks Epidermal Growth Factor (EGF)/Epidermal Growth Factor Receptor (EGFR)-Induced Biological Effects on Osteosarcoma Cells

Hua

Jiang

et al. 2018

Med Sci Monit

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BackgroundThe present work was performed to detect the potential inhibitory effect of cyclic guanosine monophosphate (cGMP)-dependent protein kinase II (PKG II) on epidermal growth factor (EGF) receptor-induced biological activity and related signal cascades in osteosarcoma cells.Material/MethodsWe transfected the osteosarcoma MG-63 cell line with an adenoviral vector encoding PKG II cDNA (Ad-PKGII) and incubated the transfected cells with 250 μM 8-pCPT-cGMP to activate the PKG II. We stimulated the MG-63 cells with100 ng/ml EGF, and then detected their proliferation using a CCK-8 assay. Transwell assay was used to examine MG-63 cell migration; and Western blot analysis was used to detect expression of matrix metalloproteinase 9 (MMP-9) and activation of ERK and Akt.ResultsStimulating cells by 100 ng/ml EGF promoted MG-63 cell proliferation and migration, ERK and Akt phosphorylation, and MMP-9 expression. These effects of EGF were inhibited in MG-63 cells infected with Ad-PKGII and incubated with 8-pCPT-cGMP.ConclusionsOur results demonstrate that Ad-PKGII infection significantly inhibited EGF-induced proliferation and migration, as well as the associated-signal cascades; which indicates that PKG II might be a potential anti-cancer factor.

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“…Given that approximately 70% of ovarian cancer cells express high levels of epidermal growth factor receptor (EGFR) and the involvement of this receptor in angiogenesis and metastasis processes, the inhibition of this receptor could offer a potential treatment for ovarian cancer [153,154]. Macrophages that are activated by weakly induced apoptosis from the TNF family cytokine (TWEAK) [155] produce exosomes that contain some more microRNA-7 (miR-7) relative to macrophages that are activated commonly.…”

Section: Inhibition Of Metastasis Migration and Proliferation In Tumo...mentioning

confidence: 99%

Immune cells-derived exosomes function as a double-edged sword: role in disease progression and their therapeutic applications

et al. 2022

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Exosomes, ranging in size from 30 to 150 nm as identified initially via electron microscopy in 1946, are one of the extracellular vesicles (EVs) produced by many cells and have been the subject of many studies; initially, they were considered as cell wastes with the belief that cells produced exosomes to maintain homeostasis. Nowadays, it has been found that EVs secreted by different cells play a vital role in cellular communication and are usually secreted in both physiological and pathological conditions. Due to the presence of different markers and ligands on the surface of exosomes, they have paracrine, endocrine and autocrine effects in some cases. Immune cells, like other cells, can secrete exosomes that interact with surrounding cells via these vesicles. Immune system cells-derived exosomes (IEXs) induce different responses, such as increasing and decreasing the transcription of various genes and regulating cytokine production. This review deliberate the function of innate and acquired immune cells derived exosomes, their role in the pathogenesis of immune diseases, and their therapeutic appliances.

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A monoclonal antibody targeting the dimer interface of epidermal growth factor receptor (EGFR)

Cited by 8 publications

References 20 publications

HCV-E2 inhibits hepatocellular carcinoma metastasis by stimulating mast cells to secrete exosomal shuttle microRNAs

HCV-E2 inhibits hepatocellular carcinoma metastasis by stimulating mast cells to secrete exosomal shuttle microRNAs

Cyclic Guanosine Monophosphate (cGMP)-Dependent Protein Kinase II Blocks Epidermal Growth Factor (EGF)/Epidermal Growth Factor Receptor (EGFR)-Induced Biological Effects on Osteosarcoma Cells

Immune cells-derived exosomes function as a double-edged sword: role in disease progression and their therapeutic applications

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