A Monoclonal Antibody Specific to Surface Antigen on Candida krusei

Abstract

A monoclonal antibody (MAb; MAb 6B3) which reacts specifically with a cell wall antigen found in all strains or isolates of Candida krusei was developed. MAb 6B3 was extensively tested by immunofluorescence assay for cross-reaction with many Candida, Cryptococcus, Saccharomyces, Trichosporon, and Rhodotorula species and was found to react only with the species C. krusei. The specific epitope is expressed on the surface of fungal cells and appears to reside on a protein moiety. Taking into account the increasing importance of fluconazoleresistant strains in nosocomial fungal infections, the very high degree of specificity of this MAb for C. krusei could be useful for the routine detection of C. krusei in culture or in tissue samples.

Evaluation of an immunomagnetic separation method to capture Candida yeasts cells in blood

“…Immunomagnetic beads (Estapor ® Merck, France) coated with MAb 5B2 or MAb 6B3 (2 mg/ml) were obtained from SR2B (Avrillé, France) [ 18 , 19 ]. MAb 5B2 recognises the predominantly polysaccharidic antigen present on the cell surface of several species of Candida ( C. albicans , C. glabrata , C. tropicalis ) [ 20 ].…”

“…It is thought that the reactive structures recognised by MAb 5B2 consist of mannopyranosyl units bound through β(1–2) linkages. MAb 6B3 reacts specifically with a cell wall surface antigen that was found to be expressed by all C. krusei strains or isolates [ 19 ].…”

New Monoclonal Antibody Specific for Candida albicans Germ Tube

“…To our knowledge, four monoclonal antibodies (MAbs) have been demonstrated by indirect immunofluorescence assay (IFA) to be specific for the C. albicans mycelial phase (8,21,22,26,28); however, only two of these components that are recognized by Western blotting have been recovered exclusively from C. albicans hyphae (8,21). Concerning species specificity, two MAbs have been isolated; the first of these is specific to C. krusei (30) and the second of these is specific to C. albicans blastoconidia (31). In this paper we present the characteristics of a novel C. albicans germ tube-specific MAb (MAb 16B1-F10) that allows differentiation of C. albicans from the recently identified species C. dubliniensis (10,37).…”