A Monoclonal Antibody Specific for the A Antigen of Brucella spp.

Abstract

Two murine monoclonal antibodies of the IgG3 class have been isolated after immunization with Brucella abortus. An indirect immunofluorescence test was used to screen hybridoma supernatants and subsequently to determine the cross-reactivity of the monoclonal antibodies with other bacteria. One monoclonal antibody reacted with all the smooth Brucella biotypes tried and with Yersinia enterocolitica serogroup 0 :9, though not with rough Br. ovis or with strains of Escherichia, Proteus, Salmonella, Pseudomonas, Francisella and Bordetella. The other monoclonal antibody displayed a high degree of specificity for brucellae carrying the A lipopolysaccharide-protein surface antigen. The implications for the diagnosis of brucellosis are discussed.

Rhizosphere interactions and siderophores

“…The specificity of the immunological reaction and the virtually unlimited quantities of the antibodies combine to provide a detection mechanism which can be used both for research and commercial purposes. Mabs have been used to detect bacterial infection of plants (De Boer et al, 1988), viruses (Underwood, 1985, fungi (Ouellette and Benhamou, 1987;Wright et al, 1986), A antigens of gram negative bacteria (Quinn et al, 1984), and structural proteins (Mutharia and Hancock, 1984). Antigens that are too small to elicit an immune response alone are attached to a larger molecule and screens are used to isolate the Mab which reacts specifically with the small antigen.…”

Monoclonal antibodies against spore antigens of Bacillus anthracis

“…with 10 9 heat inactivated B. anthracis Ames spores five days before fusion [18]. Positive clones were selected by the indirect IF method earlier described [19] but using 10 4 B. anthracis Ames spores on each multispot slide well. The one resulting hybridoma, designated E12, was cloned three times on mouse feeder cells at one cell/three wells.…”

Applications of Monoclonal Antibodies in Animal Health and Production

“…Countless years of research into improving serodiagnostic tests for bovine brucellosis culminated with the advent of MAb technology. The earliest publications in this area reported only the production of MAbs against B. abortus and its surface antigens (Holman et al, 1983;Quinn et al, 1984;Schurig et al, 1984), though these authors did discuss the potential applications of their MAbs in serodiagnosis of bovine brucellosis. Bundle etal (1984) employed utilized their BEC-specific MAbs to show that the 120 kDa structural polypeptide of BEC was in volved in both virus neutralization and haemagglutination reactions (C. F. Crouch, personal communication).…”