A Monoclonal Antibody Specific for Human Thymidine Kinase 1

Abstract

Previous research has shown that thymidine kinase 1 (TK1), a nucleotide salvage pathway enzyme, is an accurate prognostic and diagnostic tumor marker. However, the current radioisotope assay for TK1 is cumbersome and has hampered the clinical application of this diagnostic technique in cancer management. To overcome the problems of the current radioisotope assay, we have produced monoclonal antibodies (MAbs) using purified TK1 from Raji cell extract. Production and confirmation of their specificity was confirmed using Western blot, immunohistochemical staining, TK1 activity inhibition assays, and enzyme-linked immunoadsorbent assay (ELISA) techniques. Thus, in the future, these antibodies may aid in the early detection of cancer and more accurate prognosis, as well as allowing for an increased ability to study the function of TK1 in basic cellular processes.

Sensitive Nonradiometric Method for Determining Thymidine Kinase 1 Activity

“…A series of recent publications describe the production and use of antibodies against TK1 (23)(24)(25)(26)(27)(28)(29). The authors of these reports have reported several interesting results, but there are some fundamental differences and problems with immunology-based measurements of TK1.…”

“…Although immunochemical methods have been reported (23)(24)(25)(26)(27)(28)(29), a highly sensitive but convenient approach for routine measurement of serum TK1 in hematologic malignancies has not been forthcoming. Furthermore, antibody-based assays would most probably provide different clinical information than would activitybased measurements (23 ).…”

The Thymidine Kinase-1(tk-1) as a Potential Tumor Marker: Serum Levels in Serum of Patients With Solid and System Malignance Neoplasms

“…: снижение уровня маркера со-ответствовало степени редукции опухолевой массы и от-ражало эффективность лечения, а возрастание -отсут-ствие клинически значимой химиочувствительности [28]. Подобные изменения уровня ТК-1 в мониторинге ОМЛ и ОЛЛ описаны и в ряде других исследований [29][30][31][32][33][34].…”

“…Необходимо отметить, что сопоставление данных, полу-ченных разными авторами, затруднено из-за использова-ния ими принципиально разных методов выявления ТК-1, несмотря на то, что сравнение активности (радиоиммун-ный анализ) и концентрации ТК-1 (иммуноферментный метод) в СК больных ОЛЛ и здоровых лиц показало на-личие относительно высокой (но не абсолютной) корреля-ционной связи между этими показателями (коэффициент корреляции Пирсона -0,806) [30].…”

Thymidine kinase 1 – A prognostic and diagnostic indicator in ALL and AML patients

“…Sera samples from both sample groups were measured using a radioassay as well as an immunoassay employing a TK1-specific monoclonal antibody (mAb) developed in our laboratory. 8 Following a natural logarithm transformation to normalize the distribution of these data, the Student's t-test and one-way ANOVA were performed. The increase in the mean serum TK1 levels between the non-cancer control individuals and the ALL patients was statistically significant (Po0.05).…”