A monoclonal antibody recognizes a highly conserved neutralizing epitope on hemagglutinin of H6N1 avian influenza virus

He, Jijun; Hsieh, Ming-Shou; Juang, Rong-Huay; Wang, Ching-Ho

doi:10.1016/j.vetmic.2014.10.008

Cited by 13 publications

(9 citation statements)

References 34 publications

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“…The H6HA1-His coding sequence derived from the plasmid pET-32b-His-H6HA1-His (He et al, 2014) was amplified using conventional polymerase chain reaction (PCR) procedures and subcloned into the pFastBac1 baculovirus expression vector by using TA cloning (Zhou et al, 1995) ( Supplementary Fig. S1).…”

Section: Construction Of the Bi-cistronic Baculovirus Expression Vectormentioning

confidence: 99%

A secretary bi-cistronic baculovirus expression system with improved production of the HA1 protein of H6 influenza virus in insect cells and Spodoptera litura larvae

Hsieh

et al. 2018

Journal of Immunological Methods

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A bi-cistronic baculovirus expression vector was constructed to facilitate the expression, detection, and isolation of the hemagglutinin (HA) fragment HA1 of H6N1 avian influenza virus (AIV) in an insect and a culture of its cells. In this construct, the GP67sp signal peptide promoted the secretion of the recombinant protein into the culture medium, and improved protein expression and purification. Enhanced green fluorescent protein, co-expressed through an internal ribosome entry site, served as a visible reporter for protein expression detection. The hemolymph of Spodoptera litura larvae infected with the bi-cistronic baculovirus was collected for the purification of the recombinant HA1, which was found to be glycosylated, and monomeric and trimeric forms of the recombinant HA1 were identified. Proteins expressed in both the cell culture and larvae served as effective subunit vaccines for the production of antiserum against HA. The antiserum recognized the H6 subtype of AIV but not the H5 subtype.

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Section: Construction Of the Bi-cistronic Baculovirus Expression Vectormentioning

confidence: 99%

A secretary bi-cistronic baculovirus expression system with improved production of the HA1 protein of H6 influenza virus in insect cells and Spodoptera litura larvae

Hsieh

et al. 2018

Journal of Immunological Methods

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“…EB2 was specific to 2838V (He et al, 2013;He et al, 2014), and exhibited no cross-reactivity to 3233 (Fig. S1).…”

Section: Recombinant Protein and Antibody Preparationmentioning

confidence: 99%

Glycosylation at hemagglutinin Asn-167 protects the H6N1 avian influenza virus from tryptic cleavage at Arg-201 and maintains the viral infectivity

Chiu

Chang

et al. 2015

Virus Research

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“…Ichihashi et al predicted six cytotoxic T lymphocyte (CTL) epitopes from internal proteins of the H5N1 highly pathogenic avian in uenza by peptide prediction programs; three of which were protective and highly conserved among three different IAV subtypes [13]. Reverse-deriving epitopes by preparing numbers of monoclonal antibodies has been sought after by many people [14][15][16]. Li et al used four monoclonal antibodies which could neutralize the HA of H7N9, H3N2, and H9N2 to recognize novel linear epitopes by peptide-based ELISA [17].…”

Section: Introductionmentioning

confidence: 99%

Identification of a Universal Antigen Epitope of Influenza A Virus using Peptide Microarray

Wang

Sun

et al. 2020

Preprint

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Background Hemagglutinin is a major surface protein in influenza A virus (IAV), and HA2 is relative conserved among different IAVs. However, identification of broad-spectrum epitopes remain less. Results Overlapping peptides of the HA2 protein of the H5N1 IAV A/Mallard/Huadong/S/2005 were synthesized and loaded on modified silica gel film to form a microarray, and antisera against different subtypes of IAVs were used to screen universal epitopes. The selected epitope was further confirmed by western blotting using anti-peptide immune serum and viruses rescued with amino acid substitution. The results showed that 485-FYHKCDNECME-495 of the 14th peptide in HA2 had broad-spectrum binding activity with antisera against H1, H3, H4, H5, H6, H7, H8, H9, and H10 subtype IAV. Substitution of amino acids (K or D) in rescued viruses resulted in decreased serum binding, indicating that they were critical residues for serum binding activity. In Immune Epitope Database, some epitopes containing 14 − 4 peptide were confirmed as MHC-II-restricted CD4 T cell epitope and had effects on releasing IL-2 or IFN. Conclusion The identified epitope should be a novel universal target for diagnostics and its ability to generate immune protection needs further exploration.

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A monoclonal antibody recognizes a highly conserved neutralizing epitope on hemagglutinin of H6N1 avian influenza virus

Cited by 13 publications

References 34 publications

A secretary bi-cistronic baculovirus expression system with improved production of the HA1 protein of H6 influenza virus in insect cells and Spodoptera litura larvae

A secretary bi-cistronic baculovirus expression system with improved production of the HA1 protein of H6 influenza virus in insect cells and Spodoptera litura larvae

Glycosylation at hemagglutinin Asn-167 protects the H6N1 avian influenza virus from tryptic cleavage at Arg-201 and maintains the viral infectivity

Identification of a Universal Antigen Epitope of Influenza A Virus using Peptide Microarray

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