A monoclonal antibody reacting with a determinant on leptospiral lipopolysaccharide protects guinea pigs against leptospirosis

Jost, B. Helen; Adler, Ben; Vinh, Tu; Faine, S.

doi:10.1099/00222615-22-3-269

Cited by 107 publications

(109 citation statements)

References 20 publications

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“…Most of the outer envelope proteins and the low MW carbohydrate antigen were shown to be cross-reactive antigens, of which the low MW carbohydrate antigen may be useful in an early genus-reactive diagnostic test. As LPS elicits opsonic and protective antibodies [13,[27][28][29]30], lacks endotoxic activity [31] and has been shown to be a major protective antigen involved in the human antibody response to infection with other leptospiral serovars [7,9], LPS components may be useful as immunizing agents. Studies are currently being undertaken to further define the components of LPS and other antigens involved in the immune response to infection with leptospires.…”

Section: Discussionmentioning

confidence: 99%

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Antigens recognized by the human immune response to severe leptospirosis in Barbados

et al. 1991

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SUMMARYSerum samples obtained from patients hospitalized in Barbados with severe leptospirosis were tested by the microscopic agglutination test (MAT), enzyme immunoassay (EIA) and immunoblotting with leptospires that had been isolated from these patients. While serum samples taken a few days after onset of symptoms often showed no apparent correlation between MAT and EIA, later sequential serum samples produced similar profiles in both tests during the course of infection. Immunoblotting sonicate from Leptospira interrogans serovars arborea, copenhageni and bim with patients' sera, revealed reactions with a number of bands that corresponded with outer envelope components. These components included lipopolysaccharide (LPS), flagella and other outer membrane proteins, in addition to a low-molecular-weight (MW) carbohydrate cross-reactive with members of the Leptospiraceae. IgM antibodies elicited in the first to second week after infection reacted mainly with LPS and the low-MW cross-reactive carbohydrate. Comparative analysis of isolates of the same serovar by sodium dodecyl sulphate polyacrylamide gel electrophoresis and immunoblotting showed that while two serovar arborea isolates were identical, serovar bim isolates differed significantly from each other. This difference was also observed in comparative MAT testing.

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Section: Discussionmentioning

confidence: 99%

“…Monoclonal antibodies MUM/F9-10/copenhageni and MUM/F1-1/copenhageni were produced from BALB/c mice immunized with serovar copenhageni as previously described [11,13].…”

Section: Serological Tests and Monoclonal Antibodiesmentioning

confidence: 99%

Antigens recognized by the human immune response to severe leptospirosis in Barbados

et al. 1991

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“…In the recent years, the improvements in the immunization development strategy resulted in much less vaccine side effects as well as adjuvants being more widely used [26]. The transmission systems capability in activating the innate immune systems is accompanied by the transmission of antigen to lymphatic nodes [27,28].…”

Section: Discussionmentioning

confidence: 99%

“…IgM and IgG anti-bodies can be found in the serum of those who have once exposed to an acute bacteria strain up to 6 years [26]. Antibodies against bacteria lipopolysaccharide (LPS) are the first to fight Liptospira.…”

Section: Examining Adjuvants and Humeral Immune Systemmentioning

confidence: 99%

Development of Leptospira Killed Whole Culture Vaccine Using Different Adjuvants and Evaluation of Humoral Immune Response in Hamsters

Banihashemi¹,

Baradaran²,

Tebianian³

et al. 2013

J Vaccines Vaccin

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Leptospirosis is an acute infectious disease caused by Leptospira spp. that produces a broad range of health problems mainly in developing world. In several affected countries, a major attempt has been begun to find a potent and effective vaccine. This is while; the available vaccines neither perused immunological memory nor confer cross-protection. Four-week-old male hamsters were subcutaneously inoculated with four-leptospiral vaccine and four-control adjuvant through a triple-injection regiment at fortnightly intervals. Animals were bled through the saphenous vein weekly until the seventh week. We have demonstrated that adjuvant plays a fundamental role in the type and extent of the immune responses raised by leptospiral vaccination. We strongly recommend selection of alum in any formulation of leptospiral vaccine.

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“…Sempre se acreditou que a resposta humoral fosse o principal mecanismo de defesa contra a leptospirose, entretanto, há relatos na literatura que indicam uma importante participação da resposta celular na imunidade adquirida contra a leptospirose (JOST et al, 1986;FAINE, 1999;DELLAGOSTIN et al, 2011). …”

Section: Introductionunclassified

<b>Avaliação do potencial imunogênico e vacinal das flagelinas de <i>Leptospira interrogans</i> sorovar Copenhageni</b>

Monaris¹

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A monoclonal antibody reacting with a determinant on leptospiral lipopolysaccharide protects guinea pigs against leptospirosis

Cited by 107 publications

References 20 publications

Antigens recognized by the human immune response to severe leptospirosis in Barbados

Antigens recognized by the human immune response to severe leptospirosis in Barbados

Development of Leptospira Killed Whole Culture Vaccine Using Different Adjuvants and Evaluation of Humoral Immune Response in Hamsters

<b>Avaliação do potencial imunogênico e vacinal das flagelinas de <i>Leptospira interrogans</i> sorovar Copenhageni</b>

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