A Monoclonal Antibody, GR7A4, Reacting with the T10 Antigen

Ruiz‐Cabello, Francisco; Higuera, B. de la; Nevot, Miguel Ángel López; Huelin, C.; Conejo, J.M.; Garrido, Federico

doi:10.1089/hyb.1987.6.275

Cited by 5 publications

(2 citation statements)

References 15 publications

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“…The following monoclonal antibodies were used: GRB1 against DR antigen [10]; TU22 against DQ [51] and B7/21 against DP [49]; GRT2, which recognizes the common leukocyte antigen CD45 [22]; Leu 4, Leu 3a, Leu 2a, against CD3, CD4, CD8 respectively (Becton Dickinson); Bear-1 against CD1 lb [24]; OKT6 against CD1 (ORTHO); GRM1 against CD16 [40]; IOM-1 against CD20 (Immunotech); MAR 108 against the IL-2 receptor (CD25) (31), GR7A4 against CD38 [41] and FG1/8 against the 4F2 antigen [8].…”

Section: Monoclonal Antibodiesmentioning

confidence: 99%

HLA-DR expression is associated with excellent prognosis in squamous cell carcinoma of the larynx

et al. 1990

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We studied class II antigen expression and tumor-infiltrating leukocytes (TIL) in tissue sections of 69 squamous cell carcinomas of the larynx and 24 lymph node metastases in the neck. HLA-DR expression was found only in eight well-differentiated, highly keratinizing squamous cell carcinomas comprising seven of the verrucous variety and one ventriculosaccular tumor. None of the metastases was positive for DR antigen. Neither primary tumors nor autologous metastases stained for DP or DQ antigens. DR-positive tumors shared a peculiar pattern of TIL composed mainly of T cells, most of which belonged to the cytotoxic/suppressor subset, and B cells. These neoplasms had in common a slow rate of growth, and are considered low-grade carcinomas in the literature. We conclude from our study that HLA-DR expression seems to characterize tumors with a prominent infiltrate and an excellent prognosis.

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Section: Monoclonal Antibodiesmentioning

confidence: 99%

HLA-DR expression is associated with excellent prognosis in squamous cell carcinoma of the larynx

et al. 1990

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“…The T10 antigen, on the other hand, with a M, of 43 kD is only present at some stages of B-cell development and maturation and is not B-cell specific (28). T10 is also found on thymocytes and activated T lymphocytes.…”

Section: Discussionmentioning

confidence: 98%

Biochemical identification of the antigen recognized by the monoclonal pan‐B cell antibody Y29/55

Rentsch

Bucher

1991

European J of Haematology

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To examine the biochemical structure of the antigen recognized by the monoclonal pan‐B cell antibody, Y29/55, the Daudi‐ and Jurkat‐cell lines were labeled by two different methods and immunoprecipitation experiments were carried out. After surface labeling with iodine, two bands with molecular weights of about 38 and 42 kD were observed. The same two proteins were precipitated after biosynthetic labeling with (35S)‐methionine from B cells and, to a lesser extent, from T cells. Therefore, it seems that the same proteins, or proteins with similar molecular weight, exist intracellularly in T cells as exist on the surface of, and possibly intracellularly, in B cells. It was confirmed that 80–90% of normal blood‐derived B cells were stained with Y29/55 by indirect immunofluorescence. Double‐labeling experiments with the pan‐B cell antibodies Leu 16 (CD 20) and Leu 12 (CD 19) showed a B‐cell population which could be stained with both antibodies (Y29/55 and Leu 16 or Y29/55 and Leu 12). A minor cell population was stained with the antibody Y29/55 alone. Our findings indicate that the antibody Y29/55 recognizes a B‐cell antigen, which has not been described previously.

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