A monoclonal antibody-based immunosensor for the electrochemical detection of imidacloprid pesticide

Pérez-Fernández, Beatriz; Mercader, Josep V.; Checa-Orrego, Brenda I.; Escosura‐Muñiz, Alfredo de la; Costa-Garcı́a, Agustı́n

doi:10.1039/c9an00176j

Cited by 42 publications

(19 citation statements)

References 29 publications

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“…Biosensors offer a promising alternative solution for the real-time and at the point-of-need analysis of olive oil. Specifically, for the detection of pesticides a number of different biorecognition molecules (antibodies [4,5], aptamers [6]) and biomimetic elements (imprinted polymers [7,8]) have been employed towards the development of affinity sensors [9] that rely on a number of different signal transduction principles (optical, piezoelectric and even mechanical).…”

Section: Introductionmentioning

confidence: 99%

Facile and Low-Cost SPE Modification Towards Ultra-Sensitive Organophosphorus and Carbamate Pesticide Detection in Olive Oil

et al. 2020

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Despite the fact that a considerable amount of effort has been invested in the development of biosensors for the detection of pesticides, there is still a lack of a simple and low-cost platform that can reliably and sensitively detect their presence in real samples. Herein, an enzyme-based biosensor for the determination of both carbamate and organophosphorus pesticides is presented that is based on acetylcholinesterase (AChE) immobilized on commercially available screen-printed carbon electrodes (SPEs) modified with carbon black (CB), as a means to enhance their conductivity. Most interestingly, two different methodologies to deposit the enzyme onto the sensor surfaces were followed; strikingly different results were obtained depending on the family of pesticides under investigation. Furthermore, and towards the uniform application of the functionalization layer onto the SPEs’ surfaces, the laser induced forward transfer (LIFT) technique was employed in conjunction with CB functionalization, which allowed a considerable improvement of the sensor’s performance. Under the optimized conditions, the fabricated sensors can effectively detect carbofuran in a linear range from 1.1 × 10−9 to 2.3 × 10−8 mol/L, with a limit of detection equal to 0.6 × 10−9 mol/L and chlorpyrifos in a linear range from 0.7 × 10−9 up to 1.4 × 10−8 mol/L and a limit of detection 0.4 × 10−9 mol/L in buffer. The developed biosensor was also interrogated with olive oil samples, and was able to detect both pesticides at concentrations below 10 ppb, which is the maximum residue limit permitted by the European Food Safety Authority.

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Section: Introductionmentioning

confidence: 99%

Facile and Low-Cost SPE Modification Towards Ultra-Sensitive Organophosphorus and Carbamate Pesticide Detection in Olive Oil

et al. 2020

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“…Our device has also been validated with HPLC-MS/MS, the reference method used in official laboratories. Moreover, our findings represent a methodological improvement compared with indirect competitive immunoassays previously reported by our group [49] in terms of simplicity, time and cost of analysis. All this makes our immunosensor a reliable and advantageous alternative for the in field determination of IMD pesticide.…”

Section: Discussionmentioning

confidence: 59%

“…Moreover, such performance is better than that reported for alternative electrochemical methods for pesticide analysis based on direct detection and enzymatic inhibition (Table 2), having our approach also clear advantages in terms of selectivity and time/cost of analysis respectively. Comparing with our previous work based on an indirect immunosensor [49], the direct format presents clear advantages in terms of time and cost of analysis, since we avoid the use of secondary antibodies. Moreover, the increase in the electroactive area of the working electrode by the AuNPs modification may also contribute to the improvement in the limit of detection calculated.…”

Section: Optimization Of the Main Parameters Affecting The Analytical Signalmentioning

confidence: 99%

“…In our particular approach, a competitive direct assay between free IMD and IMD labelled with horseradish peroxidase (HRP) enzyme is performed. Such direct format presents clear advantages compared with indirect assays [49], avoiding the use of secondary antibodies and leading to a faster and cheaper methodology suitable for in field analysis. A JEOL 6610LV scanning electron microscope was used to characterize the sensor surface.…”

Section: Introductionmentioning

confidence: 99%

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Direct competitive immunosensor for Imidacloprid pesticide detection on gold nanoparticle-modified electrodes

Pérez-Fernández

Mercader

Abad‐Fuentes

et al. 2020

Talanta

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A direct competitive immunosensor for the electrochemical determination of Imidacloprid (IMD) pesticide on gold nanoparticle-modified screen-printed carbon electrodes (AuNP-SPCE) is here reported for the first time. Self-obtained specific monoclonal antibodies are immobilized on the AuNP-SPCE taking advantage of the AuNPs biofunctionalization abilities. In our biosensor design, free IMD in the sample competes with IMD conjugated with horseradish peroxidase (IMD-HRP) for the recognition by the antibodies. After that, 3,3′,5,5′-Tetramethylbenzidine (TMB) is enzymatically oxidized by HRP, followed by the oxidized TMB reduction back at the surface of the SPCE. This process gives an associated catalytic current (analytical signal) that is inversely proportional to the IMD amount. The main parameters affecting the analytical signal have been optimized, reaching a good precision (repeatability with a RSD of 6%), accuracy (relative error of 6%), stability (up to one month), selectivity and an excellent limit of detection (LOD of 22 pmol L -1 ), below the maximum levels allowed by the legislation, with a wide response range (50 -10000 pmol L -1 ). The detection through antibodies also allows to have an excellent selectivity against other pesticides potentially present in real samples. Low matrix effects were found when analysisng IMD in tap water and watermelon samples. The electrochemical immunosensor was also validated with HPLC-MS/MS, the reference method used in official laboratories for IMD analysis, through statistical tests. Our findings make the electrochemical immunosensor as an outstanding method for the rapid and sensitive determination of IMD at the point-of-use.

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“…Representative examples of competitive immunosensors are those based on the use of enzymatic tags proposed for the determination of Chlorsulfuron [178] and Imidacloprid [179] at levels as low as 22 pM, also taking advantage of the use of AuNPs [180] for the immobilization of the antibody. Tap water, watermelon and tomato samples were analyzed here without the need of pretreatment (Figure 6B).…”

Section: Immunosensorsmentioning

confidence: 99%

Electrochemical (Bio)Sensors for Pesticides Detection Using Screen-Printed Electrodes

2020

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Pesticides are among the most important contaminants in food, leading to important global health problems. While conventional techniques such as high-performance liquid chromatography (HPLC) and mass spectrometry (MS) have traditionally been utilized for the detection of such food contaminants, they are relatively expensive, time-consuming and labor intensive, limiting their use for point-of-care (POC) applications. Electrochemical (bio)sensors are emerging devices meeting such expectations, since they represent reliable, simple, cheap, portable, selective and easy to use analytical tools that can be used outside the laboratories by non-specialized personnel. Screen-printed electrodes (SPEs) stand out from the variety of transducers used in electrochemical (bio)sensing because of their small size, high integration, low cost and ability to measure in few microliters of sample. In this context, in this review article, we summarize and discuss about the use of SPEs as analytical tools in the development of (bio)sensors for pesticides of interest for food control. Finally, aspects related to the analytical performance of the developed (bio)sensors together with prospects for future improvements are discussed.

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A monoclonal antibody-based immunosensor for the electrochemical detection of imidacloprid pesticide

Abstract: Imidacloprid (IMD) is one of the most used pesticides worldwide as a systemic insecticide as well as for pest control and seed treatment.

Cited by 42 publications

References 29 publications

Facile and Low-Cost SPE Modification Towards Ultra-Sensitive Organophosphorus and Carbamate Pesticide Detection in Olive Oil

Facile and Low-Cost SPE Modification Towards Ultra-Sensitive Organophosphorus and Carbamate Pesticide Detection in Olive Oil

Direct competitive immunosensor for Imidacloprid pesticide detection on gold nanoparticle-modified electrodes

Electrochemical (Bio)Sensors for Pesticides Detection Using Screen-Printed Electrodes

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