A monoclonal antibody-based ELISA for the detection of circulating excretory-secretory antigens in Taenia saginata cysticercosis

Brandt, Jef; Geerts, S.; Deken, R. De; Kumar, V.; Ceulemans, F.; Brijs, Lea; Falla, N.

doi:10.1016/0020-7519(92)90148-e

Cited by 184 publications

(154 citation statements)

References 12 publications

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“…The sensitivity of the test drops from 92.3% to 12.8% when less than 50 cysticerci are present (Van Kerchoven et al 1998, Dorny et al, 2000. According to Brandt et al (1992), differences in the sensitivity of the test may be related to the permeability of the host capsule around the cysticercus, which inϐluences the amount of excretory-secretory products released into the circulation.…”

Section: Discussionmentioning

confidence: 99%

Cysticercosis in experimentally and naturally infected pigs: parasitological and immunological diagnosis

et al. 2012

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Our objective was to evaluate the diagnosis of swine cysticercosis by examining "ante mortem" (inspection of the tongue), "post mortem" (inspection and detailed necropsy) and ELISA for research in serum of antibodies (Ab-ELISA) and antigens (Ag-ELISA). Seven (7) pigs were experimentally infected orally with eggs of Taenia solium and another 10 were naturally infected. In the pigs experimentally infected, inspection of the tongue was negative in all animals, in the routine inspection detailed necropsy and cysticercis were identified in all of them. In pigs with heavy natural infection, inspection of the tongue identified cysticerci in two (20%), while at inspection with necropsy the parasites were identified in large quantities in all animals. In ELISA for antibody search (Ab-ELISA) TS-14 recombinant protein was used, and in search for antigen (Ag-ELISA) a monoclonal antibody against this protein. In animals experimentally infected, blood was collected weekly for 140 days. The Ab-ELISA identified an increase in titers of antibody to cysticerci 21 days after infection, and at the end of the experimental period six animals (86%) were positive to the test. The search for circulating antigens (Ag-ELISA) was positive in two pigs 28 to 91 days after infection. All naturally infected pigs were positive for Ag-ELISA and Ab-ELISA. The search for antibodies and antigens by ELISA in serum from 30 pigs of a local farm and without history of cysticercosis was negative. Thus, the use of TS-14 antigen in ELISA test (Ab-ELISA) can be useful for the diagnosis of cysticercosis in pigs with low infection.

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Section: Discussionmentioning

confidence: 99%

Cysticercosis in experimentally and naturally infected pigs: parasitological and immunological diagnosis

et al. 2012

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“…Sera from humans were tested in an Ag-ELISA (Brandt et al 1992) with the modifications, including pre-treatment of the sera by trichloroacetic acid and the interpretation of the results, as described by Dorny et al (2000) and Erhart et al (2002). Because a preliminary experiment, consisting in the dissection of the entire carcass of five pigs that were found positive in the Ag-ELISA, revealed that two pigs were infected with metacestodes of T. solium, another two with T. hydatigena and one pig with both parasites, it was decided not to use this test for serodiagnosis.…”

Section: Methodsmentioning

confidence: 99%

Taeniasis-cysticercosis in Southern Ecuador: assessment of infection status using multiple laboratory diagnostic tools

Rodríguez‐Hidalgo

Benítez‐Ortiz

Praet

et al. 2006

Mem. Inst. Oswaldo Cruz

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“…All assays were used following the manufacturer's recommended practices and methodologies. The bovine serum samples were tested by ELISA for the presence of Taenia metacestode circulating antigens (Brandt et al 1992;Dorny et al 2000Dorny et al , 2002, using modifications introduced by Dorny and associates (2004).…”

Section: Serological Elisa Assaysmentioning

confidence: 99%

Seroprevalence of Major Bovine-Associated Zoonotic Infectious Diseases in the Lao People's Democratic Republic

Vongxay

Conlan

Khounsy

et al. 2012

Vector-Borne and Zoonotic Diseases

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Bovine-associated zoonotic infectious diseases pose a significant threat to human health in the Lao People's Democratic Republic (Lao PDR). In all, 905 cattle and buffalo serum samples collected in northern Lao PDR in 2006 were used to determine seroprevalence of five major bovine zoonotic infectious diseases that included Taenia saginata cysticercosis, bovine tuberculosis, Q-fever, bovine brucellosis, and bovine leptospirosis. Five enzyme-linked immunosorbent assays (ELISAs) were used to test for the presence of antibodies to the diseases, except Taenia saginata, for which we tested for the presence of Taenia metacestode circulating antigens. The overall highest prevalence was for T. saginata (46.4%), with lower prevalence for Q-fever (4%), leptospirosis (3%), tuberculosis (1%), and brucellosis (0.2%). Although there were no significant differences in the proportion of seroprevalence between sex and age of the animals sampled, there were significant differences between the provincial distributions. Further studies are required to determine the seroprevalence of these infections in other locations in Lao PDR, as well as other animal species including humans, in order to develop effective prevention and control strategies. This is the first study to investigate the prevalence of bovine zoonotic infectious agents in the Lao PDR. Positivity was demonstrated for all diseases investigated, with the highest prevalence for T. saginata antigen and Coxiella burnetti antibodies. For T. saginata, there were significant differences in the provincial distribution. Approximately 16% seroprevalence of Coxiella burnetti was noted in Xayabuly Province; however, there are no clear reasons why this was the case, and further studies are required to determine risk factors associated with this observation.

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A monoclonal antibody-based ELISA for the detection of circulating excretory-secretory antigens in Taenia saginata cysticercosis

Cited by 184 publications

References 12 publications

Cysticercosis in experimentally and naturally infected pigs: parasitological and immunological diagnosis

Cysticercosis in experimentally and naturally infected pigs: parasitological and immunological diagnosis

Taeniasis-cysticercosis in Southern Ecuador: assessment of infection status using multiple laboratory diagnostic tools

Seroprevalence of Major Bovine-Associated Zoonotic Infectious Diseases in the Lao People's Democratic Republic

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