“…The indirect ELISA was performed as follows (10) : 10 mg/ mL NKD2 in coating buffer (0.05 M bicarbonate, pH 9.6) was coated in the plates overnight at 4°C. The plates were blocked with 5% skim milk at 37°C for 2 h and washed with PBST (PBS containing 0.05% Tween-20) three times.…”