A monoclonal antibody against Lates calcarifer vitellogenin and a competitive ELISA to evaluate vitellogenin induction after exposure to xenoestrogen

Prasatkaew, Witchuda; Nanthanawat, Phochit; Khongchareonporn, Nanthika; Kingtong, Sutin

doi:10.1016/j.jes.2018.05.001

Cited by 8 publications

(3 citation statements)

References 41 publications

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“…The specificity and cross-reactivity of AChE were investigated using the PAb-electric eel AChE. Proteins of interest extracted from Nile tilapia were separated using 10 % SDS-PAGE, then blotted with Trans-Blot® SD Semi-Dry Transfer Cell (Bio-Rad) according to Prasatkaew et al [10] .…”

Section: Methodsmentioning

confidence: 99%

Development of an antibody technique for acetylcholinesterase expression detection in the gill of Nile tilapia (Oreochromis niloticus) as a glyphosate-based herbicide biomarker

et al. 2022

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Section: Methodsmentioning

confidence: 99%

Development of an antibody technique for acetylcholinesterase expression detection in the gill of Nile tilapia (Oreochromis niloticus) as a glyphosate-based herbicide biomarker

et al. 2022

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“…Dioxane was evaporated, and the obtained product was dissolved in ethyl acetate and dried with Na 2 SO 4 . After evaporation of the solvent, the product, 3‐(2‐methoxy‐carbonyl‐benzene‐sulfonylamidocarbonyl) propionic acid, was recrystallized from ethyl acetate (Kolár et al., 2002; Prasatkaew et al., 2019; Simon et al., 1998). The product was purified with thin layer chromatography with the methyl alcohol, dichloromethane, and acetic acid (2:9:0.1) as the developing solvent.…”

Section: Methodsmentioning

confidence: 99%

A stable and sensitive enzyme‐linked immunosorbent assay (ELISA) for the determination of metsulfuron‐methyl residues in foods

Liu

Qin

Cao

et al. 2021

Journal of Food Science

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A hapten of metsulfuron‐methyl was successfully designed and synthesized from 2‐methylester‐phenylsulfonamide and succinic anhydride, and the polyclonal antibody against metsulfuron‐methyl was prepared by immunization procedure with the hapten–bovine serum albumin conjugate. A stable and sensitive direct competitive enzyme‐linked immunosorbent assay (dcELISA) method had been developed under the optimal conditions. The sensitivity (IC50) was 37.03 ± 1.87 µg/L, and the detection line (IC15) was 1.57 ± 0.11 µg/L. Rice, wheat, oat, flaxseed, milk, and water were chosen to study the recovery test and the recovery rates were 83.11%–117.44% . The matrix effect was eliminated by a simple dilution of the sample extracts. The results from dcELISA were well agreement with the results from HPLC–MS. It was indicated that the developed method had good accuracy and stability. It could be applied for the detection of metsulfuron‐methyl residues. It was worth mentioning that the antibody could recognize metsulfuron‐methyl and tribenuron‐methyl with cross‐reactivities of 100% and 49.72%, respectively. In order to understand the cross‐reactivity, molecular modeling including molecular alignment and electrostatic potential surfaces were introduced. It was found that the special group of metsulfuron‐methyl played an important role, especially on C3 position of the phenyl group. Practical Application A stable, sensitive, and low‐cost dc ELISA method had been developed with good accuracy and applied in the determination of metsulfuron‐methyl in foods. Molecular simulation was introduced to understand the specificity between the antibody and the analyst. It was a good method to study the cross‐reactivity between the antibody and the analyst or analogue.

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“…2b). The lower molecular weight proteins evidenced in the SDS-PAGE could correspond to breakdown products of Vtg, which is always very unstable (Vaccaro et al 2005;Prasatkaew et al 2019). Nevertheless, protease inhibitors and low temperature were used during all chromatography steps, the presence of minor bands may be due to SDS action.…”

Section: Purification Of Mullet Vitellogeninmentioning

confidence: 99%

Identification, partial characterization, and use of grey mullet (Mugil cephalus) vitellogenins for the development of ELISA and biosensor immunoassays

et al. 2019

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Vitellogenin (Vtg) has proven to be a sensitive and simple biomarker in determining sex, sexual maturity, and xenoestrogenic effects in fish. Thus, our investigation has been focused on identification, partial characterization, and quantification of grey mullet (Mugil cephalus) Vtg through the use of a variety of biochemical and immunological analytical techniques. Mullet is considered both a promising aquaculture candidate and an important species for improving sediment quality in polyculture systems. In the first part of this work, grey mullet Vtg was purified from plasma of 17b-estradiol (E2)-induced male fish by a one-step chromatographic protocol, and partially characterized. Specific polyclonal antibodies were then raised against the mullet Vtg, and both an indirect ELISA and an optical immunosensor were set up and validated to quantify plasma Vtg. The indirect ELISA and the optical immunosensor assay developed showed linear measuring in the range 56.8-1047.1 ng mL-1 and 70-739 ng mL-1 Vtg concentrations in standard solutions, respectively. The results obtained suggest that the indirect ELISA allows Vtg detection over a wide dynamic range, thus resulting more suitable for rapid and sensitive sample screening. Therefore, we suggest that the direct immunosensor is a promising tool which needs more investigation to improve the sensitivity.

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A monoclonal antibody against Lates calcarifer vitellogenin and a competitive ELISA to evaluate vitellogenin induction after exposure to xenoestrogen

Cited by 8 publications

References 41 publications

Development of an antibody technique for acetylcholinesterase expression detection in the gill of Nile tilapia (Oreochromis niloticus) as a glyphosate-based herbicide biomarker

Development of an antibody technique for acetylcholinesterase expression detection in the gill of Nile tilapia (Oreochromis niloticus) as a glyphosate-based herbicide biomarker

A stable and sensitive enzyme‐linked immunosorbent assay (ELISA) for the determination of metsulfuron‐methyl residues in foods

Identification, partial characterization, and use of grey mullet (Mugil cephalus) vitellogenins for the development of ELISA and biosensor immunoassays

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