A monoclonal antibody against annexin A2 targets stem and progenitor cell fractions in tumors

Kalra, Rajkumar S.; Soman, Gaurav S.; Parab, Pradeep B.; Mali, Avinash M.; Varankar, Sagar; Naik, Rutika R.; Kamble, Swapnil C.; Dhanjal, Jaspreet Kaur; Bapat, Sharmila A.

doi:10.1016/j.tranon.2021.101257

Cited by 2 publications

(2 citation statements)

References 42 publications

(64 reference statements)

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“…Similarly, the expression of visual cycle and retinoid uptake genes was significantly reduced (log 2 FC < −1; padj < 0.05) in cluster C1 relative to other clusters ( Table 4 , Figure 4 D). We also examined genes known to contribute to SP cell maintenance and renewal or are differentially upregulated in SP cells, including Aldoc , Dkk3 , Id3 , Tmsb4x [ 91 ], Anxa2 [ 92 ], Nbl1 [ 93 ], Rax [ 94 ], and Rarres2 [ 75 ]. Transcripts from these genes were more abundant in cluster C1 ( Figure 4 B, Table 5 ) and declined in C2–6.…”

Section: Resultsmentioning

confidence: 99%

Single-Cell RNA Sequencing Reveals Molecular Features of Heterogeneity in the Murine Retinal Pigment Epithelium

Pandey

Krebs

Bolisetty

et al. 2022

IJMS

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Transcriptomic analysis of the mammalian retinal pigment epithelium (RPE) aims to identify cellular networks that influence ocular development, maintenance, function, and disease. However, available evidence points to RPE cell heterogeneity within native tissue, which adds complexity to global transcriptomic analysis. Here, to assess cell heterogeneity, we performed single-cell RNA sequencing of RPE cells from two young adult male C57BL/6J mice. Following quality control to ensure robust transcript identification limited to cell singlets, we detected 13,858 transcripts among 2667 and 2846 RPE cells. Dimensional reduction by principal component analysis and uniform manifold approximation and projection revealed six distinct cell populations. All clusters expressed transcripts typical of RPE cells; the smallest (C1, containing 1–2% of total cells) exhibited the hallmarks of stem and/or progenitor (SP) cells. Placing C1–6 along a pseudotime axis suggested a relative decrease in melanogenesis and SP gene expression and a corresponding increase in visual cycle gene expression upon RPE maturation. K-means clustering of all detected transcripts identified additional expression patterns that may advance the understanding of RPE SP cell maintenance and the evolution of cellular metabolic networks during development. This work provides new insights into the transcriptome of the mouse RPE and a baseline for identifying experimentally induced transcriptional changes in future studies of this tissue.

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Section: Resultsmentioning

confidence: 99%

Single-Cell RNA Sequencing Reveals Molecular Features of Heterogeneity in the Murine Retinal Pigment Epithelium

Pandey

Krebs

Bolisetty

et al. 2022

IJMS

View full text Add to dashboard Cite

show abstract

“…Similarly, the expression of visual cycle and retinoid uptake genes was significantly reduced (log2FC < -1; padj < 0.05) in cluster C1 relative to other clusters (Table 4, Figure 4 D). We also examined genes known to contribute to stem/progenitor cell maintenance and renewal or are differentially upregulated in stem/progenitor cells, including Aldoc, Dkk3, Id3, Tmsb4x [51], Anxa2 [52], Nbl1 [53], Rax [54], 5) and declined in C2-6. Overall, these results support the identification of C1 as a stem/progenitor cell population and the proposed maturation of RPE cells from C1 to C6.…”

Section: A Proposed Cluster Maturation Timelinementioning

confidence: 99%

Single-cell RNA sequencing reveals molecular features of postnatal maturation in the murine retinal pigment epithelium

Pandey

Krebs

Bolisetty

et al. 2022

Preprint

View full text Add to dashboard Cite

Transcriptomic analysis of the mammalian retinal pigment epithelium (RPE) aims to identify cellular networks that influence ocular development, maintenance, function, and disease. However, available evidence points to RPE cell heterogeneity in the native tissue, which adds complexity to transcriptomic analysis. Here, to assess cell heterogeneity, we performed single-cell RNA sequencing of RPE cells from two young adult male C57BL/6J mice. Following quality control to ensure robust transcript identification limited to cell singlets, we detected 13,858 transcripts among 2,667 and 2,846 RPE cells, respectively. Dimensional reduction by principal component analysis and uniform manifold approximation and projection revealed six distinct cell popu-lations. All clusters expressed transcripts typical of RPE cells; the smallest (C1, containing 1–2% of total cells) exhibited hallmarks of stem and/or progenitor cells. Placing C1–6 along a pseudotime axis suggested a relative decrease in melanogenesis and stem/progenitor gene expression, and a corresponding increase in visual cycle gene expression upon RPE maturation. K-means testing of all detected transcripts identified additional expression patterns that may advance understanding of RPE stem/pro-genitor cell maintenance and the evolution of cellular metabolic networks during development. This work provides new insights into the transcriptome of the mouse RPE and a baseline for identifying experimentally induced transcriptional changes in future studies of this tissue.

show abstract

A monoclonal antibody against annexin A2 targets stem and progenitor cell fractions in tumors

Cited by 2 publications

References 42 publications

Single-Cell RNA Sequencing Reveals Molecular Features of Heterogeneity in the Murine Retinal Pigment Epithelium

Single-Cell RNA Sequencing Reveals Molecular Features of Heterogeneity in the Murine Retinal Pigment Epithelium

Single-cell RNA sequencing reveals molecular features of postnatal maturation in the murine retinal pigment epithelium

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