A monoclonal anti-DC1 antibody selectivity inhibits the generation of effector T cells mediating specific cytolytic activity.

Corte, Giorgio; Moretta, A.; Cosulich, Maria Elisabetta; Ramarli, Dunia; Bargellesi, A.

doi:10.1084/jem.156.5.1539

Cited by 57 publications

(15 citation statements)

References 11 publications

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“…In this respect, it has been found that only the DR+, DC+ and not DR+, DC-subpopulation of human monocytes is able to function as APC for autologous T lymphocytes (87) and that DC molecules can play a role in the generation of cytotoxic lymphocytes in an MLC (88). Following these observations, it was of interest to determine first the relative expression of DR and DC products on Me cells, and then the possibility to modulate them by IFN-y.…”

Section: Expression Of Class II Antigens On Me Cells and Their Role Imentioning

confidence: 99%

Autologous cellular immune response to primary and metastatic human melanomas and its regulation by DR antigens expressed on tumor cells

et al. 1985

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Evidence for heterogeneity of several biological features of human malignant melanoma (Me) like morphology, cytogenetics, oncogenes activation, antigenic expression, metastatizing capacity and procoagulant activity are briefly reviewed in an attempt to distinguish findings related to primary vs. metastatic lesions. In our own studies monoclonal antibodies were used to study expression of MHC class I, class II products and of Me-associated antigens (MAA) on primary and metastatic Me cells. High expression of class I antigens was found in a high percentage of both primary and metastatic tumors, whereas DR and MAA showed a significant variation (from 3 to 90% of cells) in expression both in primary and in metastatic Me. When autologous cell-mediated immune responses were evaluated, it was found that Me cells from primary tumors but not those from lymph node metastases were able to stimulate autologous lymphocytes to proliferate and become cytotoxic for autologous Me. Clonal analysis of cytotoxic lymphocytes was then carried out in order to see whether the lack of lymphocytes reactivity to metastatic cells was due to the absence or to a low frequency of cytotoxic cells in the unstimulated PBL. CTL clones cytotoxic for autologous Me (Auto-Me) cells were indeed isolated. Three classes of CTL clones were identified: 1) one which is cytotoxic for Auto-Me; 2) a second one which lyse Auto-Me and allogeneic Me; and 3) a third one which is cytotoxic for Auto-Me and allogeneic normal and neoplastic cells. Metastatic Me cells, however, had the ability to suppress the stimulation of autologous PBL by alloantigens or IL-2. This effect was dose-dependent and was not due to absorption of IL-2 by Me cells. Since it has been reported that Me cells express class II MHC antigens, we investigated whether there was any correlation between autologous immune responses and DR expression on Me cells. Autologous lymphocytes stimulation was found to occur only with DR+ Me cells from primary lesions, whereas metastatic cells, either DR+ or DR-, did not stimulate autologous PBL. Moreover, the suppressive effect of metastatic Me cells was associated with their expression of DR antigens. The modulation of DR antigens on Me cells by Interferon-gamma correlated positively with their suppressive capacity. Thus, it appears that primary Me can behave differently from the metastatic one in their interactions with the immune system of autologous host. These findings suggest that DR antigens on Me cells may have an important role in the regulation of autologous immune responses.

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Section: Expression Of Class II Antigens On Me Cells and Their Role Imentioning

confidence: 99%

Autologous cellular immune response to primary and metastatic human melanomas and its regulation by DR antigens expressed on tumor cells

et al. 1985

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“…Further dissociation of the functional properties of HLA-DR and HLA-DQ antigens have been demonstrated. Corte et al (1982) showed that an anti-DQwl Moab was capable of blocking the production of specific cytotoxic cells in MLC. No blocking was obtained with a DR Moab.…”

Section: Figure3 Dna Extracted From the Htcsmentioning

confidence: 99%

Different functions and associations of HLA‐DR and HLA‐DQ(DC) antigens shown by serological, cellular and DNA assays

et al. 1985

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Two consanguineous Caucasoid HTCs, DHI and FPA, were investigated, the latter having an unusual HLA-DR/DQ(DC) association. Both these HTCs typed clearly as HLA-DRwll. However, while DHI typed as DRwll/DQw3(DC4) as expected, FPA typed as DRwll/DQwl(DCl) instead. Although extremely rare in Caucasoids, DRwll/DQwl is a common pattern of association in Nigerian Negroids. Southern blots of DNA extracted from EBV cell line derived from FPA, hybridized with HLA-DCa and HLA-DCP probes, confirmed this unusual DRwll/DQwl(DC1) association. In addition the DCa probe showed a unique additional restriction fragment length polymorphism (8 kb) attributable to the DX gene in the FPA DNA. When DHI and FPA were used as stimulators in MLC, the patterns to typing responses obtained were not completely concordant although they overlap to some extent. For this reason FPA has been locally designated Dw'FS', distinct from Dw5. Furthermore, the HLA-DQ antigens of the responder cells were not necessarily the same as those of the HTCS to which they gave typing responses (FPA and DHI). Functional studies using these two HTCs showed that the DQ(DC) antigens probably have no direct lymphocute activating properties but rather have a regulatory role in controlling responses to allodeterminants in MLC.

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“…The results of Sasazuki et al (1984) on suppressor T cell dependent low responsiveness to Streptococcal antigens associated with DQwl and data from Corte et al (1982) on the inhibition of cytotoxic T cells with the help of a monoclonal anti-DC1 (DQwl) antibody support the latter (suppressorkytotoxic) hypothesis. If DQwl is involved in the DRw6 donor effect, we would expect the same phenomenon with the D R 1 and DR2 positive grafts which are also in most cases DQwl positive.…”

Section: The Drw6 Donor Effectmentioning

confidence: 89%

The regulatory role of HLA‐DRw6 in renal transplantation

et al. 1986

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A monoclonal anti-DC1 antibody selectivity inhibits the generation of effector T cells mediating specific cytolytic activity.

Cited by 57 publications

References 11 publications

Autologous cellular immune response to primary and metastatic human melanomas and its regulation by DR antigens expressed on tumor cells

Autologous cellular immune response to primary and metastatic human melanomas and its regulation by DR antigens expressed on tumor cells

Different functions and associations of HLA‐DR and HLA‐DQ(DC) antigens shown by serological, cellular and DNA assays

The regulatory role of HLA‐DRw6 in renal transplantation

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