A molecularly imprinted polymer for the determination of neopterin

Sole, Roberta Del; Scardino, Anna; Lazzoi, Maria Rosaria; Mergola, Lucia; Scorrano, Sonia; Vasapollo, Giuseppe

doi:10.1007/s00604-013-0982-y

Cited by 11 publications

(13 citation statements)

References 32 publications

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“…One of the attractive features of the MIPs is their use in different scientific fields and a wide range of target molecules have been successfully used.…”

Section: Introductionmentioning

confidence: 99%

A molecularly imprinted polymer as artificial receptor for the detection of indole‐3‐carbinol

Scorrano

Mergola

Sole

et al. 2014

J of Applied Polymer Sci

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Molecularly imprinting polymer technology is used to prepare a molecularly imprinted polymer (MIP) for the selective recognition of indole-3-carbinol (I3C), a chemopreventive and chemotherapeutic phytochemical associated with the anticancer activities of cruciferous vegetables. Prepolymerization study via nuclear magnetic resonance technique is done to choose the best functional monomer that establishes more interaction with the template. The prepared MIP is tested before in batch experiments and subsequently used as solid-phase extraction sorbent for the selective detection of I3C from standard solutions. In order to verify the selectivity of the MIP, the binding of structurally related compounds, such as indole-3-acetonitrile, teophylline, and tryptophan, on the polymer is investigated. The experiments indicate that the MIP is highly selective for I3C with an association constant of K a 5 (1.37 6 0.07) 3 10 3 M 21 . Standard mixture solution loaded on MIP-SPE cartridge give a recovery of 95% for I3C, while the other compounds are totally eluted during washing step.

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“…One of the attractive features of the MIPs is their use in different scientific fields and a wide range of target molecules have been successfully used.…”

Section: Introductionmentioning

confidence: 99%

A molecularly imprinted polymer as artificial receptor for the detection of indole‐3‐carbinol

Scorrano

Mergola

Sole

et al. 2014

J of Applied Polymer Sci

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“…Starting from Del Sole and co-workers’ expertise in the field of imprinting technology [ 12 , 13 , 16 , 19 , 21 , 22 ] and using the labeling reaction composition as starting knowledge, the design of a purification method of [ 11 C]RAC employing molecularly imprinted polymeric particles was considered advantageous in terms of simplicity and reliability of radiopharmaceutical manufacturing. Assuringly, in recent years several groups have published numerous works demonstrating the use of MIT in the preparation of selective systems for the extraction of important compounds [ 13 , 15 , 18 , 23 , 24 ]. Therefore, we prepared MIPs selective for [ 11 C]RAC and DM-RAC using non-radioactive templates and standard approaches.…”

Section: Resultsmentioning

confidence: 99%

“…Furthermore, RAC imprinted polymer features specific cavities and functional groups positioned in a well-defined spatial arrangement reflecting the tridimensional structure of RAC, promoting a much stronger interaction of this compound with the polymer compared to DM-RAC ( Figure 6 ). Indeed, the main advantage of MIT is to discriminate between very similar molecules that differ only for their spatial conformation (chiral molecules) [ 27 , 28 , 29 ] or small functional groups [ 13 , 14 , 30 , 31 ]. In this case, DM-RAC differs structurally from RAC only in the presence of a proton instead of a methyl group.…”

Section: Resultsmentioning

confidence: 99%

“…After polymerization, the template is removed with several washing steps, leaving in the polymer-specific recognition sites that are complementary in size and shape to the template. In this way, an intelligent synthetic system with high selectivity towards the template can be generated [ 12 , 13 , 14 , 15 , 16 ]. The imprinted polymer obtained, compared to the biological system, often used for its high recognition capacity, has inertness towards acidic, alkaline, and organic solvents but also high robustness and resistance to elevated temperature and pressure.…”

Section: Introductionmentioning

confidence: 99%

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Raclopride-Molecularly Imprinted Polymers: A Promising Technology for Selective [11C]Raclopride Purification

et al. 2023

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In this work, we developed a novel approach to purify [11C]Raclopride ([11C]RAC), an important positron emission tomography radiotracer, based on tailored shape-recognition polymers, with the aim to substitute single-pass HPLC purification with an in-flow trap & release process. Molecular imprinting technology (MIT) applied to solid phase extraction (MISPE) was investigated to develop a setting able to selectively extract [11C]RAC in a mixture containing a high amount of its precursor, (S)-O-Des-Methyl-Raclopride (DM-RAC). Two imprinted polymers selective for unlabeled RAC and DM-RAC were synthesized through a radical polymerization at 65 °C using methacrylic acid and trimethylolpropane trimethacrylate in the presence of template molecule (RAC or DM-RAC). The prepared polymer was characterized by scanning electron microscopy and Fourier transform infrared spectroscopy and tested in MISPE experiments. The polymers were used in testing conditions, revealing a high retention capacity of RAC-MISPE to retain RAC either in the presence of similar concentrations of RAC and DM-RAC precursor (96.9%, RSD 6.6%) and in the presence of a large excess of precursor (90%, RSD 4.6%) in the loading solution. Starting from these promising results, preliminary studies for selective purification of [11C]Raclopride using this RAC-MISPE were performed and, while generally confirming the selectivity capacity of the polymer, revealed challenging applicability to the current synthetic process, mainly due to high backpressures and long elution times.

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“…The template is added during the polymerization process and removed by washing procedures, leaving complementary cavities with respect to shape and size in the polymer network. In this way the polymer can exhibit high affinity towards the template molecule that can be selectively re‐bound to specific sites …”

Section: Introductionmentioning

confidence: 99%

Towards a new strategy of a chitosan‐based molecularly imprinted membrane for removal of 4‐nitrophenol in real water samples

Bello

Mergola

Scorrano

et al. 2017

Polymer International

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The issue of water contaminants, which affects human and environmental health, is not trivial. It is thus paramount to find new cheap and user friendly ways to detect and remove them from the environment. Here, the synthesis of a green chitosan (CS) based molecularly imprinted membrane for the detection and quantification of 4‐nitrophenol (4‐NO2Ph) in aqueous media is proposed. The concentration of 4‐NO2Ph in a water solution was measured by HPLC analysis. CS as a functional polymer, 4‐NO2Ph as template, 4‐[(4‐hydroxy)phenylazo]benzenesulfonic acid as ligand, and glutaraldehyde as crosslinker in the presence of polyethylene glycol as porogen were used. The membrane was characterized by SEM and Fourier transform IR analyses, which confirmed the CS and polyethylene glycol backbone of the membrane. Kinetic studies of the detection system were performed by using pseudo‐first‐order and pseudo‐second‐order models. Then, the binding efficiency between 195.33 µmol L−1 and 9235.55 µmol L−1 of 4‐NO2Ph was evaluated, finding a maximum adsorption of 723.25 µmol 4‐NO2Ph per gram of membrane consistent with the Qmax calculated from the Langmuir isotherm. The selectivity of the membrane versus three phenolic competitor molecules, sharing very similar molecular structure to 4‐NO2Ph, was demonstrated. Finally, the applicability of the membrane to real‐world samples was evaluated, by using drinking water spiked with 7.19 µmol L−1 of 4‐NO2Ph, obtaining a removal efficiency of 70.6%. © 2017 Society of Chemical Industry

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A molecularly imprinted polymer for the determination of neopterin

Cited by 11 publications

References 32 publications

A molecularly imprinted polymer as artificial receptor for the detection of indole‐3‐carbinol

A molecularly imprinted polymer as artificial receptor for the detection of indole‐3‐carbinol

Raclopride-Molecularly Imprinted Polymers: A Promising Technology for Selective [11C]Raclopride Purification

Towards a new strategy of a chitosan‐based molecularly imprinted membrane for removal of 4‐nitrophenol in real water samples

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