A Molecularly Imprinted Polymer for the Reconstruction of a Molecular Recognition Region

Murakami, Sachiko; Shinmori, Hideyuki; Takeuchi, Toshio

doi:10.1246/cl.2008.1028

Cited by 19 publications

(11 citation statements)

References 6 publications

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“…Namely, hierarchically imprinted SiO 2 could cooperatively bind both Cu 2+ and the surfactant. There was also reported as cofactor effect in MIP 18. The cooperative binding of substrate and porphyrin cofactor was observed in enantioselectivity of MIPs.…”

Section: Introductionmentioning

confidence: 88%

Molecular imprinting micropolymerbeads having cooperative effect of both surfactant and inosine template

Kusunoki¹,

Kobayashi²

2010

J of Applied Polymer Sci

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Molecular imprinted polymer (MIP) microbeads were prepared by emulsion copolymerization of divinylbenzene (DVB) and methacrylic acid (MAA) in the presence of inosine (INO) template and laurylbenzenesulfonic acid (LBSA) as surfactant. The polymerization was carried out at 55 C under ultrasound exposure. The resulting copolymer microbeads, having 0.1-0.4 lm diameter, were observed to have a binding behavior of INO and surfactant to the polymer which was strongly dependent of the bulk pH; for example, at pH 3, 6, and 10 values of binding for INO to the imprinted copolymer were 3.7 lmol/g, 2.1 lmol/g, and 0 lmol/g, respectively.It was found that LBSA surfactant bound to the MIP microbeads similarly depending on pHs. At pH 3, 6, and 10, the LBSA values of binding were 23 lmol/g, 1.3 lmol/g, and 4.8 lmol/ g. It was also noted that the surfactant binding was enhanced in the presence of the INO template. This demonstrated that a cooperative binding with the surfactant was cooperatively occurred in the presence of INO.

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Section: Introductionmentioning

confidence: 88%

Molecular imprinting micropolymerbeads having cooperative effect of both surfactant and inosine template

Kusunoki¹,

Kobayashi²

2010

J of Applied Polymer Sci

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“…We have developed a novel way to introduce a fluorescent reporter molecule to the binding sites in hydrogel thin films by using a newly designed functional monomer 1 and a postimprinting treatment (McNiven et al, 1997;Mukawa et al, 2002; Murakami et al, 2008;Takeda et al, 2009;Takeuchi et al, 2006;Yane et al, 2006) with an amino-reactive fluorescent dye (Fig. 1).…”

Section: Introductionmentioning

confidence: 99%

Fluorescent protein recognition polymer thin films capable of selective signal transduction of target binding events prepared by molecular imprinting with a post-imprinting treatment

Sunayama

Ooya

Takeuchi

2010

Biosensors and Bioelectronics

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“…Previously, we demonstrated successfully that multifunctional synthetic materials prepared by molecular imprinting with well‐designed template molecules or functional monomers, and multistep PIMs, achieved the transformation of functional groups . The method also achieved the introduction of additional functions in the imprinted cavities, such as on/off switching of binding activity, fluorescent signaling for readout of specific binding events, catalytic functions, and other desirable features . To the best of our knowledge, there have been no reports revealing the regulation of the protein‐binding activity of MIPs via postchemical modification, with the exception of our previous studies, which provided only partial demonstrations …”

Section: Introductionmentioning

confidence: 87%

Regulation of protein‐binding activities of molecularly imprinted polymers via post‐imprinting modifications to exchange functional groups within the imprinted cavity

Sunayama

Kitayama

Takeuchi

2017

J of Molecular Recognition

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We prepared lysozyme-imprinted polymers bearing modifiable sites within the imprinted cavity to introduce various functional groups via post-imprinting modifications. For this purpose, ({[2-(2-methacrylamido)-ethyldithio]-ethylcarbamoyl}-methoxy)acetic acid (MDTA), which has a carboxy group to interact with the target protein, lysozyme, and a disulfide linkage for postimprinting modifications, was used as a functional monomer. A lysozyme-imprinted polymer film was prepared by copolymerization of MDTA with a cross-linker, N,N′-methylenebisacrylamide, in the presence of lysozyme. After removing lysozyme, followed by reducing the disulfide linkage, various functional groups, such as carboxy, amino, sulfonate, and oligo-ethylene oxide, were introduced to the exposed thiol groups via a disulfide exchange reaction using the pyridyldisulfide derivatives of these functional groups. Various functional groups could be introduced reversibly via this post-imprinting disulfide exchange reaction after the construction of the lysozymeimprinted cavities. The modification regulated the protein-binding activity. The proposed post-imprinting modification system, based on a molecular imprinting process, is expected to lead to the development of advanced materials for fine-tuning and/or introducing desired functions.

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A Molecularly Imprinted Polymer for the Reconstruction of a Molecular Recognition Region

Cited by 19 publications

References 6 publications

Molecular imprinting micropolymerbeads having cooperative effect of both surfactant and inosine template

Molecular imprinting micropolymerbeads having cooperative effect of both surfactant and inosine template

Fluorescent protein recognition polymer thin films capable of selective signal transduction of target binding events prepared by molecular imprinting with a post-imprinting treatment

Regulation of protein‐binding activities of molecularly imprinted polymers via post‐imprinting modifications to exchange functional groups within the imprinted cavity

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