A molecularly imprinted electrochemical sensor based on surface imprinted polymerization and boric acid affinity for selective and sensitive detection of P-glycoproteins

Yang, Huayu; Song, Hua; Suo, Ziqin; Li, Fu-Nan; Jin, Quanyi; Zhu, Xuan; Chen, Qing

doi:10.1016/j.aca.2022.339797

Cited by 16 publications

(5 citation statements)

References 17 publications

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“…Compared to these materials, microorganisms have advantages such as low cost, easy availability, and the presence of abundant active biomolecules on the cell wall, eliminating the need for further modification processes. Pravit et al [61] developed a stable water-in-oil Pickering emulsion based on a bacterial chitosan network, which opened up opportunities for the development of environmentally friendly novel interface materials and the use of bacterial cell networks in novel microreactors.…”

Section: Yeast's Involvement As External and Internal Supporting Subs...mentioning

confidence: 99%

Molecularly Imprinted Polymers Using Yeast as a Supporting Substrate

Wang,

Dong,

Shen

et al. 2023

Molecules

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Molecularly imprinted polymers (MIPs) have gained significant attention as artificial receptors due to their low cost, mild operating conditions, and excellent selectivity. To optimize the synthesis process and enhance the recognition performance, various support materials for molecular imprinting have been explored as a crucial research direction. Yeast, a biological material, offers advantages such as being green and environmentally friendly, low cost, and easy availability, making it a promising supporting substrate in the molecular imprinting process. We focus on the preparation of different types of MIPs involving yeast and elaborate on the specific roles it plays in each case. Additionally, we discuss the advantages and limitations of yeast in the preparation of MIPs and conclude with the challenges and future development trends of yeast in molecular imprinting research.

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Section: Yeast's Involvement As External and Internal Supporting Subs...mentioning

confidence: 99%

Molecularly Imprinted Polymers Using Yeast as a Supporting Substrate

Wang,

Dong,

Shen

et al. 2023

Molecules

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“…Yang et al produced a P-glycoprotein SIP with a LoD of 22 fg/L. However, this approach faces the challenges of on-surface cross-linking [19]. Polymerizing and crosslinking reagents can interfere with the biomarker structure, whilst milder processes, such as UV cross-linkable materials, are often water soluble [14].…”

Section: Introductionmentioning

confidence: 99%

A Surface Imprinted Polymer EIS Sensor for Detecting Alpha-Synuclein, a Parkinson’s Disease Biomarker

Massey,

Appadurai,

Prakash

2024

Micromachines

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Parkinson’s Disease (PD) is a debilitating neurodegenerative disease, causing loss of motor function and, in some instances, cognitive decline and dementia in those affected. The quality of life can be improved, and disease progression delayed through early interventions. However, current methods of confirming a PD diagnosis are extremely invasive. This prevents their use as a screening tool for the early onset stages of PD. We propose a surface imprinted polymer (SIP) electroimpedance spectroscopy (EIS) biosensor for detecting α-Synuclein (αSyn) and its aggregates, a biomarker that appears in saliva and blood during the early stages of PD as the blood-brain barrier degrades. The surface imprinted polymer stamp is fabricated by low-temperature melt stamping polycaprolactone (PCL) on interdigitated EIS electrodes. The result is a low-cost, small-footprint biosensor that is highly suitable for non-invasive monitoring of the disease biomarker. The sensors were tested with αSyn dilutions in deionized water and in constant ionic concentration matrix solutions with decreasing concentrations of αSyn to remove the background effects of concentration. The device response confirmed the specificity of these devices to the target protein of monomeric αSyn. The sensor limit of detection was measured to be 5 pg/L, and its linear detection range was 5 pg/L–5 µg/L. This covers the physiological range of αSyn in saliva and makes this a highly promising method of quantifying αSyn monomers for PD patients in the future. The SIP surface was regenerated, and the sensor was reused to demonstrate its capability for repeat sensing as a potential continuous monitoring tool for the disease biomarker.

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“…To overcome the obstacle of recognizing constituents and to minimize the cost, molecularly imprinted polymers (MIPs) have drawn much attention thanks to their capacity as artificial antibodies with a better performance in stability, more predictability in structure and an easier preparation process [ 13 ]. Based on covalent and non-covalent bonds between functional monomers and templates, the polymerization proceeds around templates.…”

Section: Introductionmentioning

confidence: 99%

A Label-Free and Antibody-Free Molecularly Imprinted Polymer-Based Impedimetric Sensor for NSCLC-Cells-Derived Exosomes Detection

et al. 2023

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In this study, a label-free and antibody-free impedimetric biosensor based on molecularly imprinting technology for exosomes derived from non-small-cell lung cancer (NSCLC) cells was established. Involved preparation parameters were systematically investigated. In this design, with template exosomes anchored on a glassy carbon electrode (GCE) by decorated cholesterol molecules, the subsequent electro-polymerization of APBA and elution procedure afforded a selective adsorption membrane for template A549 exosomes. The adsorption of exosomes caused a rise in the impedance of the sensor, so the concentration of template exosomes can be quantified by monitoring the impedance of GCEs. Each procedure in the establishment of the sensor was monitored with a corresponding method. Methodological verification showed great sensitivity and selectivity of this method with an LOD = 2.03 × 103 and an LOQ = 4.10 × 104 particles/mL. By introducing normal cells and other cancer cells derived exosomes as interference, high selectivity was proved. Accuracy and precision were measured, with an obtained average recovery ratio of 100.76% and a resulting RSD of 1.86%. Additionally, the sensors’ performance was retained at 4 °C for a week or after undergoing elution and re-adsorption cycles seven times. In summary, the sensor is competitive for clinical translational application and improving the prognosis and survival for NSCLC patients.

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A molecularly imprinted electrochemical sensor based on surface imprinted polymerization and boric acid affinity for selective and sensitive detection of P-glycoproteins

Cited by 16 publications

References 17 publications

Molecularly Imprinted Polymers Using Yeast as a Supporting Substrate

Molecularly Imprinted Polymers Using Yeast as a Supporting Substrate

A Surface Imprinted Polymer EIS Sensor for Detecting Alpha-Synuclein, a Parkinson’s Disease Biomarker

A Label-Free and Antibody-Free Molecularly Imprinted Polymer-Based Impedimetric Sensor for NSCLC-Cells-Derived Exosomes Detection

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