A molecular systematic survey of cultured microbial associates of deep-water marine invertebrates

Sfanos, Karen S.; Harmody, Dedra; Dang, Phat; Ledger, A.; Pomponi, Shirley A.; McCarthy, Peter J.; Lopez, Jose V.

doi:10.1016/j.syapm.2004.12.002

Cited by 108 publications

(73 citation statements)

References 54 publications

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“…Our results and those of others indicate that Stappia strains are readily isolated from many marine environments (1,5,9,17,27,33). Though Stappia strains have been considered "marine" based on sodium requirements and weak growth in nonmarine media (1,27,38), three of four isolates in this study (strains GA15, MIO, and M4) exhibit growth optima in diluted seawater (Table 3).…”

Section: Discussionsupporting

confidence: 63%

“…Stappia stellulata and Stappia aggregata were originally isolated from coastal marine water column and sediment samples, assigned to the genus Agrobacterium, and subsequently transferred to the genus Stappia (1,29,38). Stappia alba (27), Stappia marina (9), and various Stappia-like isolates have since been obtained from numerous widely distributed sources, including warm temperate surface and permanently cold deep-sea waters, sediments, phytoplankton, macroalgae, invertebrates, and salt marshes (2,3,5,9,13,17,26,27,33; Donachie et al, unpublished data). In addition, the presence of Stappia or Stappia-like taxa in a similar range of habitats has also been inferred from cultivation-independent analyses (3,26;Donachie et al,unpublished).…”

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confidence: 99%

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Physiological, Ecological, and Phylogenetic Characterization of Stappia , a Marine CO-Oxidizing Bacterial Genus

Weber

King

2007

Appl Environ Microbiol

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Bacteria play a major role in marine CO cycling, yet very little is known about the microbes involved. Thirteen CO-oxidizing Stappia isolates obtained from existing cultures, macroalgae, or surf samples representing geographically and ecologically diverse habitats were characterized using biochemical, physiological, and phylogenetic approaches. All isolates were aerobic chemoorganotrophs that oxidized CO at elevated (1,000 ppm) and ambient-to-subambient concentrations (<0.3 ppm). All contained the form I (OMP) coxL gene for aerobic CO dehydrogenase and also the form II (BMS) putative coxL gene. In addition, some strains possessed cbbL, the large subunit gene for ribulose-1,5-bisphosphate carboxylase/oxygenase, suggesting the possibility of lithotrophic or mixotrophic metabolism. All isolates used a wide range of sugars, organic acids, amino acids, and aromatics for growth and grew at salinities from 5 to 45 ppt. All but one isolate denitrified or respired nitrate. Phylogenetic analyses based on 16S rRNA gene sequences indicated that several isolates could not be distinguished from Stappia aggregata and contributed to a widely distributed species complex. Four isolates (of strains GA15, HI, MIO, and M4) were phylogenetically distinct from validly described Stappia species and closely related genera (e.g., Ahrensia, Pannonibacter, Pseudovibrio, and Roseibium). Substrate utilization profiles, enzymatic activity, and membrane lipid composition further distinguished these isolates and supported their designations as new Stappia species. The observed metabolic versatility of Stappia likely accounts for its cosmopolitan distribution and its ability to contribute to CO cycling as well as other important biogeochemical cycles.

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Section: Discussionsupporting

confidence: 63%

mentioning

confidence: 99%

Physiological, Ecological, and Phylogenetic Characterization of Stappia , a Marine CO-Oxidizing Bacterial Genus

Weber

King

2007

Appl Environ Microbiol

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“…Isolation of Vibrio spp. from sponges was carried out as previously described (Sfanos et al, 2005). Genomic DNA was isolated from pure Vibrio cultures using the ZR Fungal/Bacterial DNA kit (Zymo Research, Orange, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

Population dynamics of Vibrio spp. associated with marine sponge microcosms

et al. 2010

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Vibrio is a diverse genus of marine-associated bacteria with at least 74 species and more expected as additional marine ecospheres are interrogated. This report describes a phylogenetic reconstruction of Vibrio isolates derived from one such unique ecosystem, marine sponges (Phylum Porifera) collected from depths of 150 to 1242 feet. 16S rRNA gene sequencing along with molecular typing of 16S-23S rRNA intergenic spacer regions clustered many sponge-associated Vibrio (spp) with current known species. That is, several benthic Vibrio species commensal with Porifera sponges seemed genetically linked to vibrios associated with coastal or shallow-water communities, signalling a panmictic population structure among seemingly ecologically disparate strains. Conversely, phylogenetic analysis provided evidence for at least two novel Vibrio speciation events within this specific sponge microcosm. Collectively, these findings earmark this still relatively unknown environment as a bastion of taxonomic and phylogenetic variability for the genus and probably other bacterial taxa.

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“…Nrs. DQ889910, AF489287; [25,26]). Alcanivorax-like organisms have also been detected in a few terrestrial environments whose characteristics share relevant properties (salinity, presence of hydrocarbons) with marine ecosystems, e.g.…”

Section: Biogeography Of Ohcbmentioning

confidence: 99%