A molecular study on Theileria and Babesia in cattle from Isfahan province, Central Iran

Babesia species are protozoan parasites that parasitize the erythrocytes of domestic animals and humans, causing anemia in the host affected. These parasites cause a zoonotic disease known as babesiosis. Polymerase chain reaction (PCR) has proven to be very sensitive for detecting Babesia in blood samples of affected animals, particular in ruminants. The purpose of the current study was to determine the presence of Babesia DNA in the blood samples obtained from cattle, camel and sheep in Iran. In addition, the study aimed at establishing a rapid, reliable, specific and sensitive molecular tool, the PCR, for the detection of Babesia DNA in ruminants and dromedaries. Blood samples were collected from 372 ruminants and dromedaries (155 cattle, 95 sheep and 122 camel) kept at the Livestock Experimental Station. The animals came from randomly selected herds located in the important livestock-production regions of Iran of Isfahan and Chaharmahal va Bakhtiary during December 2012 to March 2013. PCR was used to detect Babesia DNA in the blood samples whereby an amplified band size of 428 bp was considered positive for Babesia spp. The results indicated that 7.10% (n= 155), 6.56% (n= 122) and 0.00% (n= 95) of the blood samples from cattle, camel and sheep were positive for Babesia DNA, respectively. The findings from this study revealed that there were Babesia DNA in blood taken from cattle and camel. To our knowledge, this is the first report to show the presence of Babesia DNA in blood samples of Iranian ruminants and dromedaries in Chaharmahal Va Bakhtiari and Isfahan provinces by PCR method. Though, diagnosis of low-level infections by the parasite is important for the epidemiological studies. Our findings support the power of PCR test for Babesia DNA detection in blood samples and could be easily used for routine diagnosis.

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“…and none of them was positive for Babesia spp. (Noaman, 2013). These findings are contrary to the present study.…”

Section: Discussioncontrasting

confidence: 99%

Determination of the presence of Babesia species in blood samples of cattle, camel and sheep in Iran by PCR

Khamesipour¹,

Doosti²,

Koohi³

et al. 2015

ARCH BIOL SCI BELGRA

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“…The forward and reverse primers used in the present study was previously designed as a Theileria genus specific primer based on hyper variable region V4 of 18S rRNA gene sequences for the detection of this parasite in the carrier animals (Shayan and Rahbari 2005). Previously, the prevalence of Theileria infections in cattle was determined in central region of Iran using PCR (Noaman 2012) but sensitivity and specificity of microscopic technique in comparison with PCR is not evaluated yet.…”

Section: Discussionmentioning

confidence: 99%

Comparison of molecular and microscopic technique for detection of Theileria spp. in carrier cattle

Noaman

2012

J Parasit Dis

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In Iran, theileriosis is normally diagnosed with traditional Giemsa staining method. This is not applicable for identification of the carrier animals. The aim of this study was to compare conventional Giemsa staining method with the PCR technique in the detection of Theileria organisms. In this study, examinations were performed on 150 blood samples from cattle without clinical signs. Sensitivity and specificity of 50 microscopic fields were compared with Theileria specific PCR. The degree of agreement between PCR and microscopic test was determined by Kappa (j) values with 95 % confidence intervals. PCR showed that 42 samples were Theileria spp. positive, while routine microscopy showed erythrocytes harboring Theileria like structures in 11 blood samples. Examination of 50 microscopic fields showed 57 % sensitivity and 99 % specificity compared to 100 % sensitivity and specificity for PCR. The j coefficient between PCR and Microscopy (50 fields) techniques indicated no level of agreement. Our results showed that the microscopic examination remains the convenient technique for day-to-day diagnosis of clinical cases in the laboratory but for the detection of carrier animal containing low parasitemia. Therefore, molecular methods such as PCR can be used as a safe method for identifying cattle persistently infected with Theileria spp.

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“…Recently, PCR and semi-nested PCR were used for detection of Babesia and Theileria spp. of the 18S ribosomal ribonucleic acid (18S-rRNA) gene (Heidarpour Bami et al 2010;Noaman 2013;Motavalli Haghi et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Detection of Babesia infection among human, goats and sheep using microscopic and molecular methods in the city of Kuhdasht in Lorestan Province, West of Iran

2017

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is a lethal protozoan disease, responsible for the loss of livestock in Iran and in the world. The purpose of the current study was to detect and identify spp. infection using microscopic and molecular methods in human, sheep and goats in Kuhdasht region, in the Lorestan Province, west of Iran. During 2013, a total of 384 blood smear samples were collected from 51 goats, 306 sheep suspected of infection and 27 humans from Kuhdasht region. The blood samples were fixed, stained and under light microscopic examined. DNA samples were extracted and amplified by polymerase chain reaction of 18S-rRNA gene. PCR and the semi-nested PCR were performed to identify to and to differentiate genus of and spp. The results of microscopic examination indicated that a total of 47 (12.2%) samples were positive for spp. infection: 38 (9.9%) belonging to sheep and 9 to goats (2.3%). No was observed in human samples. The PCR showed a band size of 389 bp, of spp. and the semi-nested PCR detected with a band size of 186 bp. By molecular method, 16 (4.2%) sheep and 2 (0.5%) goat blood samples were infected by. Totally, 18 samples (4.7%) were observed to have a, while no infection was found in human. Thus, the results of our study have shown sheep and goats could be vulnerable to spp., especially in Lorestan Province, Iran. Therefore, studies on the status of the animal especially are recommended.

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A molecular study on Theileria and Babesia in cattle from Isfahan province, Central Iran

Abstract: Bovine theileriosis and babesiosis are important hemoprotozoal diseases of cattle in tropical and subtropical regions that lead to economic losses in these animals. From

Cited by 7 publications

References 12 publications

Determination of the presence of Babesia species in blood samples of cattle, camel and sheep in Iran by PCR

Determination of the presence of Babesia species in blood samples of cattle, camel and sheep in Iran by PCR

Comparison of molecular and microscopic technique for detection of Theileria spp. in carrier cattle

Detection of Babesia infection among human, goats and sheep using microscopic and molecular methods in the city of Kuhdasht in Lorestan Province, West of Iran

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