A molecular study of dormancy breaking and germination in seeds of Trollius ledebouri

Bailey, Paul; Lycett, Grantley W.; Roberts, Jeremy A.

doi:10.1007/bf00019110

Cited by 15 publications

(6 citation statements)

References 38 publications

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“…However, the results indicate that Cp82 is not certainly related to flower-bud dormancy. The dormancy-related protein CAA93825.1, which matched Cp82 ( E -value = 1e −15 ), has been reported to play a role in dormancy breaking and in the germination of Trollius ledebourii seeds [37]. These data warrant further research into the relativity of Cp82 to dormancy breaking and germination in C. praecox seeds.…”

Section: Resultsmentioning

confidence: 84%

Generation and Analysis of Expressed Sequence Tags fromChimonanthus praecox(Wintersweet) Flowers for Discovering Stress-Responsive and Floral Development-Related Genes

Sui

Luo

et al. 2012

Comparative and Functional Genomics

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A complementary DNA library was constructed from the flowers ofChimonanthus praecox, an ornamental perennial shrub blossoming in winter in China. Eight hundred sixty-seven high-quality expressed sequence tag sequences with an average read length of 673.8 bp were acquired. A nonredundant set of 479 unigenes, including 94 contigs and 385 singletons, was identified after the expressed sequence tags were clustered and assembled. BLAST analysis against the nonredundant protein database and nonredundant nucleotide database revealed that 405 unigenes shared significant homology with known genes. The homologous unigenes were categorized according to Gene Ontology hierarchies (biological, cellular, and molecular). By BLAST analysis and Gene Ontology annotation, 95 unigenes involved in stress and defense and 19 unigenes related to floral development were identified based on existing knowledge. Twelve genes, of which 9 were annotated as “cold response,” were examined by real-time RT-PCR to understand the changes in expression patterns under cold stress and to validate the findings. Fourteen genes, including 11 genes related to floral development, were also detected by real-time RT-PCR to validate the expression patterns in the blooming process and in different tissues. This study provides a useful basis for the genomic analysis ofC. praecox.

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Section: Resultsmentioning

confidence: 84%

Generation and Analysis of Expressed Sequence Tags fromChimonanthus praecox(Wintersweet) Flowers for Discovering Stress-Responsive and Floral Development-Related Genes

Sui

Luo

et al. 2012

Comparative and Functional Genomics

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“…Many of these products have sequence homology with proteins involved in desiccation tolerance or protection against various injuries and with storage proteins [9][10][11][12][13][14]. Identifying these functions is important for understanding the differences between the dormant and non-dormant states, but to date no 'switch' function that could be involved in the choice between maintenance and release of dormancy and germinating has been discovered by these approaches.…”

Section: The Physiology Of Seed Germinationmentioning

confidence: 99%

Untitled

Bove¹,

Jullien²,

Grappin³

2001

Genome Biol

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A recent proteomic analysis of germinating Arabidopsis thaliana seeds demonstrates the effectiveness of functional genomics for investigating the complexity of developmental regulatory networks, such as the development of the embryo into a young plant. The electronic version of this article is the complete one and can be found online at http://genomebiology.com/2001/3/1/reviews/1002 © BioMed Central Ltd (Print ISSN 1465-6906; Online ISSN 1465-6914) In flowering plant development, seed germination is the transition of the quiescent embryo, which has developed from the fertilized ovule, into a new photosynthetically active plant. The visible sign that germination has been completed is the protrusion of the radicle, the precursor of the root, through the seed coat; germination sensu stricto begins, however, with water uptake by the seed (imbibition) and ends with the start of elongation the embryonic axis inside the seed [1]. Germination results from a combination of many cellular and metabolic events, coordinated by a complex regulatory network that includes seed dormancy, an intrinsic ability to temporarily block radicle elongation in order to optimize the timing of germination [2]. In the field of seed biology, germination mechanisms and their control by dormancy have been investigated in a wide range of species. Nonetheless, how these processes are coordinated, how they contribute to germination, and the regulatory network leading to completion of germination remain poorly understood.The availability of the complete genome sequence of the model plant Arabidopsis thaliana [3], together with the development of high-throughput procedures for global analyses of gene function, has launched the 'post-genomic' era of plant biology. Systematic analyses of RNA and protein expression patterns, and of post-translational modifications, are now feasible for a large set of genes [4]. These can provide important clues about protein-protein interactions and gene functions in a complex developmental context. A recent proteome study of germinating Arabidopsis seeds [5] highlights the effectiveness of using this model organism to provide information on germination that may prove general to all plants. The physiology of seed germinationIn temperate climates, most mature seeds, consisting of an embryo surrounded by endosperm and a seed coat (testa), are dispersed from the mother plant in a state of low moisture content (5-15%) and with metabolic activity at a standstill. Some physiologists have hypothesized that with regard to their structure, genetic information and macromolecular content, dry seeds are in a state of readiness to resume metabolism [6]. For germination to occur, quiescent seeds need only be hydrated under conditions that encourage metabolism, such as a suitable temperature and the presence of oxygen (Figure 1). The uptake of water by the seed, which is considered to be a trigger for germination, and the metabolic processes that take place as a result, are described in Figure 1. Seed germination can be de...

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“…Several studies using cDNA-library screening, differential display and cDNA macro-and micro-arrays approaches (Ried and Walker-Simmons, 1990;Goldmark et al, 1992;Johnson et al, 1995;Li and Foley, 1995;Bailey et al, 1996;Stacy et al, 1996;Lorenzo et al, 2001Lorenzo et al, , 2002aPotokina et al, 2002;Ogawa et al, 2003) as well as proteomic approaches (Gallardo et al, 2001(Gallardo et al, , 2002 have identified many transcripts or proteins whose accumulation during imbibition correlate with dormancy expression or seed germination. However, many of these genes seem to be involved in desiccation tolerance, seed protection against injury, DNA repair, growth metabolism, or germination completion.…”

Section: Introductionmentioning

confidence: 99%

Gene expression analysis by cDNA-AFLP highlights a set of new signaling networks and translational control during seed dormancy breaking in Nicotiana plumbaginifolia

Bove

Lucas²,

Godin³

et al. 2005

Plant Mol Biol

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Seed dormancy in Nicotiana plumbaginifolia is characterized by an abscisic acid accumulation linked to a pronounced germination delay. Dormancy can be released by 1 year after-ripening treatment. Using a cDNA-amplified fragment length polymorphism (cDNA-AFLP) approach we compared the gene expression patterns of dormant and after-ripened seeds, air-dry or during one day imbibition and analyzed 15,000 cDNA fragments. Among them 1020 were found to be differentially regulated by dormancy. Of 412 sequenced cDNA fragments, 83 were assigned to a known function by search similarities to public databases. The functional categories of the identified dormancy maintenance and breaking responsive genes, give evidence that after-ripening turns in the air-dry seed to a new developmental program that modulates, at the RNA level, components of translational control, signaling networks, transcriptional control and regulated proteolysis.

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A molecular study of dormancy breaking and germination in seeds of Trollius ledebouri

Cited by 15 publications

References 38 publications

Generation and Analysis of Expressed Sequence Tags fromChimonanthus praecox(Wintersweet) Flowers for Discovering Stress-Responsive and Floral Development-Related Genes

Generation and Analysis of Expressed Sequence Tags fromChimonanthus praecox(Wintersweet) Flowers for Discovering Stress-Responsive and Floral Development-Related Genes

Untitled

Gene expression analysis by cDNA-AFLP highlights a set of new signaling networks and translational control during seed dormancy breaking in Nicotiana plumbaginifolia

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