A molecular phylogeny of the tribe Aphidini (Insecta: Hemiptera: Aphididae) based on the mitochondrial tRNA/COII, 12S/16S and the nuclear EF1α genes

Abstract

Abstract. A phylogeny of the tribe Aphidini (Hemiptera: Aphididae) was reconstructed from three gene fragments: two mitochondrial regions, partial tRNA-leucine þ cytochrome oxidase II (tRNA/COII), partial 12S rRNA þ tRNA-valine þ 16S rRNA (12S/16S) and one nuclear gene, the elongation factor-1 alpha (EF1a). Bayesian phylogenetic (BP) analyses were performed on each individual dataset of tRNA/COII, 12S/16S and EF1a, and maximum parsimony (MP), Bremer support test, maximum likelihood (ML) and BP analysis were performed on the combined dataset. After comparing our molecular phylogenetic results with the classic classification based on morphological and ecological data, we analysed three main issues: the monophyletic relationships among tribes and subtribes, the validities of the latest taxonomic positions of genera and species and the status of certain Aphis species groups. Our results indicate that 36 of the species analysed, with the exception of Cryptosiphum artemisiae, are clustered within the clade of Aphidini. Also, the 28 species representative of the subtribe Aphidina were separated from the eight species representative of Rhopalosiphina; each monophyletic subtribe was supported by significant P-values in the combined analysis. According to our results, Cryptosiphum should be moved to Macrosiphini because it is more closely related to the genera Lipaphis and Brevicoryne. The genus Toxoptera was recovered as nonmonophyletic. In Rhopalosiphina, three genera, Hyalopterus, Rhopalosiphum and Schizaphis, were relatively closer to each other than to the genus Melanaphis. In the relationships between species-groups among Aphis, most species were separated into two main lineages; the fabae group seemed to be more closely related to the spiraecola and craccivora group rather than to the gossypii group.

Molecular and Morphological Identification of the Soybean Aphid and OtherAphisSpecies on the Primary HostRhamnus davuricain Asia

“…In addition, DNA barcode studies have accumulated the sequences of Ϸ650 bp of COI for Ͼ300 species, making molecular identiÞcation workable for aphids (Foottit et al 2008). In the genus Aphis, the molecular identiÞcation technique has effectively solved the identiÞcation problems due to cryptic morphological differences between A. gossypii and A. frangulae (Carletto et al 2009), and the phylogenetic studies have provided many useful sequences of major taxa (von Dohlen and Teulon 2003, Coeur dÕacier et al 2007, Kim and Lee 2008.…”

Evolution of Soybean Aphid Biotypes: Understanding and Managing Virulence to Host-Plant Resistance

“…This is especially true for the 3-4 species groups: A. fabae, A. craccivora, A. gossypii and A. frangulae, with the latter 2 groups sometimes lumped together as the A. gossypii group (Kim & Lee, 2008). Kim & Lee (2008) placed A. glycines relatively basal within the A. gossypii group and to A. glycines itself, based on mitochondrial and nuclear DNA.…”

“…craccivora, A. gossypii and A. frangulae, with the latter 2 groups sometimes lumped together as the A. gossypii group (Kim & Lee, 2008). Kim & Lee (2008) placed A. glycines relatively basal within the A. gossypii group and to A. glycines itself, based on mitochondrial and nuclear DNA. However, Wu et al (2004) report that A. glycines and A. gossypii can produce viable offspring in the laboratory and in nature, as well as share an overwintering host, Rhamnus davurica Pal.…”

Taxonomic review and morphometric analysis of the genusMelanaphisvan der Goot (Hemiptera: Aphididae) in Korea

“…However, monoecious species on secondary hosts seem to have lost their primary hosts. It is suggested that Melanaphis is the most basal group of Rhopalosiphina and is thus regarded to provide some clues to the evolution of host alternation in the subfamily Aphidinae (Kim and Lee 2008).…”