A Molecular Peptide Beacon for the Ratiometric Sensing of Nucleic Acids

Wu, Junchen; Zou, Ying; Li, Chunyan; Sicking, Wilhelm; Piantanida, Ivo; Yi, Tao; Schmuck, Carsten

doi:10.1021/ja2103845

Cited by 150 publications

(99 citation statements)

References 30 publications

(19 reference statements)

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“…The binding of the peptide to DNA groove may facilitate the chromophore to be intercalated into the stacks of base pairs as reported in our previous report. 33 More binding evidence came from fluorometric titration experiments with increasing DNA concentrations. Addition of CT DNA (0-50 µM) to the solution of pep3-NP1 (2.5 µM) caused a significant enhancement of the red fluorescence intensity at 646 nm with a slight red-shift (5 nm, λ ex = 455 nm) (Figure 2b).…”

Section: Resultsmentioning

confidence: 99%

Mitochondria-Directed Fluorescent Probe for the Detection of Hydrogen Peroxide near Mitochondrial DNA

et al. 2015

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It is important to detect hydrogen peroxide (H2O2) near mitochondrial DNA (mtDNA) because mtDNA is more prone to oxidative attack than nuclear DNA (nDNA). In this study, a mitochondria-targeted fluorescence probe, pep3-NP1, has been designed and synthesized. The probe contains a DNA-binding peptide, a H2O2 fluorescence reporter, and a positively charged red emissive styryl dye to facilitate accumulation in mitochondria. Due to groove binding of the peptide with DNA, the styryl dye of pep3-NP1 intercalated into the bases of DNA, leading to an increase in red fluorescence intensity (centered at 646 nm) and quantum yield. In this case, pep3-NP1 was a turn-on probe for labeling DNA. Subcellular locations of pep3-NP1 and MitoTracker suggested that pep3-NP1 mostly accumulated in the mitochondria of live cells. Namely, as an intracellular DNA marker, pep3-NP1 bound to mtDNA. In the presence of H2O2, pep3-NP1 emitted green fluorescence (centered at 555 nm). Thus, the ratio of green with red fluorescence of pep3-NP1 was suitable to reflect the change of the H2O2 level near mtDNA in living cells. The detecting limit for H2O2 was estimated at 2.9 and 5.0 μM in vitro and in cultured cells, respectively. The development of pep3-NP1 could help in studies to protect mtDNA from oxidative stress.

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Section: Resultsmentioning

confidence: 99%

Mitochondria-Directed Fluorescent Probe for the Detection of Hydrogen Peroxide near Mitochondrial DNA

et al. 2015

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“…Ratiometric fluorescent methods, which detect the analyte by measuring the change of the ratios of photoluminescence (PL) intensities at two wavelengths, have received intense attention in recent years (Wu et al, 2012;Tyrakowski et al, 2014;Yan et al, 2014a).…”

Section: Introductionmentioning

confidence: 99%

A ratiometric fluorescent quantum dots based biosensor for organophosphorus pesticides detection by inner-filter effect

Yan

Han

et al. 2015

Biosensors and Bioelectronics

230

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“…Based on this intercalative mechanism, many cationic planar, polycyclic dyes derived from quinolizinium, benzooxazolinium, benzothiazolinium, and acridizinium have been developed as powerful optical probes for DNA detection [9,10]. These DNA-selective probes demonstrate a variety of potential applications in pharmaceutical studies and molecular biology, and can be used as probes for electron and/or energy transfer and gene modulators [11][12][13][14][15][16][17][18][19][20][21].…”

Section: Introductionmentioning

confidence: 99%

A Novel Styryldehydropyridocolinium Homodimer: Synthesis and Fluorescence Properties Upon Interaction with DNA

et al. 2015

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A novel homodimer of the styryldehydropyridocolinium dye (TPTP) has been synthesized and characterized. Free TPTP exhibited low fluorescence quantum yield and large Stokes shift (over 160 nm) in water. However, it showed a significant fluorescence turn-on effect upon intercalation into DNA base pairs. Meanwhile, the fluorescence intensity of the intercalated structures formed by TPTP and DNA decreased quickly upon addition of deoxyribonuclease I, indicating that the dye can be used to monitor deoxyribonuclease I activity and DNA hydrolysis. Electrophoresis analysis revealed that the dye had intercalative binding to DNA and can potentially be used for DNA staining in electrophoresis. Thus, the innate nature of large Stokes shift and excellent fluorescence turn on effect upon interaction with DNA endue the dye with a wide range of applications.

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A Molecular Peptide Beacon for the Ratiometric Sensing of Nucleic Acids

Cited by 150 publications

References 30 publications

Mitochondria-Directed Fluorescent Probe for the Detection of Hydrogen Peroxide near Mitochondrial DNA

Mitochondria-Directed Fluorescent Probe for the Detection of Hydrogen Peroxide near Mitochondrial DNA

A ratiometric fluorescent quantum dots based biosensor for organophosphorus pesticides detection by inner-filter effect

A Novel Styryldehydropyridocolinium Homodimer: Synthesis and Fluorescence Properties Upon Interaction with DNA

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