A Molecular Marker Map for Roses

Abstract

In addition to an existing core map for diploid roses which comprised 305 molecular markers 60 additional markers were mapped to extend the map. As a first application of the information contained in the map, the map position of a resistance gene from roses, Rdr1, was determined by identifying closely linked markers on linkage group 1. Furthermore 21 microsatellite markers could be assigned to six of the seven linkage groups, therefore allowing their more efficient use in variety fingerprinting and general mapping in the near future. Apart from being a useful tool for genetic studies and map based cloning, the map could also be used in projects on the chromosome evolution within the rosaceae family.

Towards a unified genetic map for diploid roses

“…The largest cluster is located around the black spot locus (Rdr1) on LG1. This locus determines the resistance against D. rosae race DortE4 (Debener et al 2001). Within 10 cM around the black spot locus, Wve RGA markers were mapped.…”

Multiple Models for Rosaceae Genomics

“…almond (Joobeur et al, 1998;Bliss et al, 2002;Aranzana et al, 2003;Dirlewanger et al, 2004b), P. davidiana (Foulongne et al, 2003), P. cerasifera (Dirlewanger et al, 2004a), and P. ferganensis , have been developed. Maps for apple (Hemmat et al, 1994;Maliepaard et al, 1998;Liebhard et al, 2003aLiebhard et al, , 2003bLiebhard et al, , 2003cKenis and Keulemans, 2005;Naik et al, 2006;SilfverbergDilworth et al, 2006), pear (Yamamoto et al, 2004), raspberry (Graham et al, 2004), diploid strawberry (Davis and Yu, 1997;Sargent et al, 2004Sargent et al, , 2006Cipriani et al, 2006), cultivated octoploid strawberry (LerceteauKohler et al, 2003;Weebadde et al, 2008), and rose (Debener and Mattiesch, 1999;Debener et al, 2001;Rajapakse et al, 2001;Yan et al, 2005) are also available. Many of these genetic maps were constructed for particular breeding goals using specific mapping populations, resulting in multiple genetic maps of various lengths that vary in degree of saturation and type of markers.…”

Microsatellite DNA marker inheritance indicates preferential pairing between two highly homologous genomes in polyploid and hemisexual dog-roses, Rosa L. Sect. Caninae DC.

“…By contrast, the remaining five offspring were identical to the maternal parent also at this locus, in accordance with their putatively apomictic origin. The occurrence of only four RhEO506 alleles in R. dumalis and the five apomictically derived offspring Table 1 Chromosomal location of microsatellite DNA marker loci and the number of alleles found in a screening material consisting of 30 rootstock varieties (representing various species) and 46 R. hybrida varieties (Debener et al, 2001;Esselink et al, 2003) ND=not determined. Polymorphism in the investigated crosses, R. dumalis  R. rubiginosa and its reciprocal (D  R) and R. sherardii  R. villosa and its reciprocal (S  V), is indicated.…”