A molecular key to the common species of Trichogramma of the Mediterranean region

Sumer, Fahriye; Tunçbilek, Aydin; Öztemiz, Sevcan; Pintureau, Bernard; Rugman‐Jones, Paul F.; Stouthamer, Richard

doi:10.1007/s10526-009-9219-8

Cited by 54 publications

(50 citation statements)

References 25 publications

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“…However, the small size of these parasitoids (<1 mm long) and lack of clear diagnostic features make then difficult to identify, and this confusion can impede the implementation of effective biological control programs (Smith & Hubbes, 1986;Pinto et al, 1989;Pinto & Stouthamer 1994). Given the difficulties in distinguishing species of the genera, Trichogramma and Telenomus, molecular studies have gained importance in recent years (Yuliarti et al, 2002;Sümer et al, 2009;Poorjavad et al, 2012;Nasir et al, 2013). Therefore, with the advent of molecular techniques, DNA-based approaches have been used to generate molecular markers that are useful for the characterization of closely related or cryptic species for biological control work (Landry et al, 1993;Hoy et al, 2000;Chang at al., 2001).…”

Section: Feza Can Cengiz 1 Introductionmentioning

confidence: 99%

“…Since standard morphological methods are not always sufficiently precise to differentiate micro-hymenopteran to species level (Landry et al, 1993) genetic means have become the tools of choice for the routine identification of Trichogramma spp. (Sümer et al, 2009). The use of a standardized DNA region for fast and reliable species identification, such as cytochrome c oxidase I (COI), has proven to be efficient in hyperdiverse groups for which morphological identification is difficult or impossible (Valentini et al, 2009).…”

Section: Feza Can Cengiz 1 Introductionmentioning

confidence: 99%

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First record of the egg parasitoids of Chilo partellus (Swinhoe, 1885) (Lepidoptera: Crambidae) in Turkey unveiled by DNA Barcoding

Can

Kaya

Ulaşli

et al. 2016

Turkish Journal of Entomology

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Chilo partellus (Swinhoe) (Lepidoptera: Crambidae) is an invasive insect species attacking maize (Zea mays L.) and other cereal crops causing important yield losses. The occurrence of this insect in Turkey was first reported in maize growing areas of some provinces in the East Mediterranean region of Turkey in 2014. Chemical or other pest control methods do not always provide acceptable control of this pest, so biological control is considered an important alternative. However, for a successful biological control, the first step is to reliably identify the natural enemies of a target pest, which is difficult to achieve using methods based on morphology. Recent developments in molecular techniques allow more reliable identification of insect species and their parasitoids. Therefore, the aim of this study was to identify the egg parasitoids of C. partellus by molecular methods. Parasitized eggs were collected from maize fields in Hatay province, Turkey, from September to October 2014 and in September 2015. Eggs were maintained in the laboratory and emerging adult parasitoids were subjected to molecular analysis. Using DNA barcoding, two native natural enemies, Trichogramma brassicae Bezdenko (Hymenoptera: Trichogrammatidae) and Telenomus busseolae (Gahan) (Hymenoptera: Platygastridae) were identified as egg parasitoids of C. partellus for the first time in Turkey.

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Section: Feza Can Cengiz 1 Introductionmentioning

confidence: 99%

Section: Feza Can Cengiz 1 Introductionmentioning

confidence: 99%

First record of the egg parasitoids of Chilo partellus (Swinhoe, 1885) (Lepidoptera: Crambidae) in Turkey unveiled by DNA Barcoding

Can

Kaya

Ulaşli

et al. 2016

Turkish Journal of Entomology

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“…Vo e g e l é , 1 9 8 2 (Hymenoptera: Trichogrammatidae) son avispas microhimenopteras que se encuentran entre las especies más empleadas como parasitoides de huevos de plagas agrícolas en los programas de control biológico (García-González et al, 2005;Pratissoli et al, 2005;España-Luna et al, 2006;Sumer et al, 2009;Buchori et al, 2010) y se encuentran presentes en diversas zonas agroecológicas del Perú (Iannacone & Lamas, 2003a;Virgala & Botto, 2010).…”

Section: R I C H O G R a M M A P I N T O Iunclassified

Efecto Tóxico Agudo Y Crónico De Tagetes Minuta “Huacatay” (Asteraceae) Y Carbaril Sobre Seis Entomófagos De Importancia en Control Biológico

Iannacone¹,

Alvariño²,

Guabloche³

et al. 2017

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“…사육하는 종이 완전히 바뀌어 방사할 환경에 부적합한 종이 투 입될 수 있는 이유 등으로 Trichogramma속에 대한 정확한 종 동정은 생물적 방제의 성공을 위해 필요하다 Sumer et al, 2009). 그러나 Trichogramma spp.는 체장 이 1 mm 이하의 소형곤충이면서 외부 형태적으로 종 구별이 매 우 어려운 것으로 잘 알려져 있다 (Pinto and Stouthamer, 1994).…”

Section: 방제하고자 하는 해충에 대해 효과가 좋은 기생벌 선발이 중요 한데 대량사육 과정에서 목적하지 않은 종이 섞unclassified

Molecular Identification of Trichogramma (Hymenoptera: Trichogrammatidae) Egg Parasitoids of the Asian Corn Borer Ostrinia furnacalis, Based on ITS2 rDNA Sequence Analysis

Seo¹,

Jung²,

Park³

et al. 2014

Korean journal of applied entomology

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To identify the species of Trichogramma occurring in the corn fields of Korea as egg parasitoids of Ostrinia furnacalis, we sequenced the full-length of ITS2 nuclear rDNA from 112 parasitoids collected during this study. As a reference to distinguish species, we also retrieved full-length ITS2 sequences of 60 Trichogramma species from the NCBI GenBank database. On the basis of the size and 3′terminal sequence pattern of the ITS2 sequences, the Trichogramma samples collected in this study were divided into three groups (K-1, -2, and -3). Evolutionary distances (d) within and between groups based on ITS2 sequences were estimated to be ≤0.005 and ≥0.080, respectively. In the net average distance between groups or species, the d value between K-1 and T. ostriniae, K-2 and T. dendrolimi, and K-3 and T. confusum was the lowest, with values of 0.016, 0.001, and 0.002, respectively. In the phylogenetic tree, K-1 and K-2 were clustered with T. ostriniae and T. dendrolimi, respectively. However, K-3 was clustered with three different species, namely, T. confusum, T. chilonis, and T. bilingensis. NCBI BLAST results revealed that parasitoids belonging to K-1 and K-2 showed 99% identity with T. ostriniae and T. dendrolimi, respectively. Parasitoids in K-3 collected from Hongcheon showed 99-100% identity with T. confusum and T. chilonis, and one parasitoid in K-3 collected from Gochang had 98% identity with T. bilingensis, T. confusum, and T. chilonis. On the basis of these results, we infer that the species of Trichogramma collected in this study are closely related to T. ostriniae (K-1) and T. dendrolimi (K-2). However, it was not possible to distinguish species of K-3 using the ITS2 sequence alone.

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A molecular key to the common species of Trichogramma of the Mediterranean region

Cited by 54 publications

References 25 publications

First record of the egg parasitoids of Chilo partellus (Swinhoe, 1885) (Lepidoptera: Crambidae) in Turkey unveiled by DNA Barcoding

First record of the egg parasitoids of Chilo partellus (Swinhoe, 1885) (Lepidoptera: Crambidae) in Turkey unveiled by DNA Barcoding

Efecto Tóxico Agudo Y Crónico De Tagetes Minuta “Huacatay” (Asteraceae) Y Carbaril Sobre Seis Entomófagos De Importancia en Control Biológico

Molecular Identification of Trichogramma (Hymenoptera: Trichogrammatidae) Egg Parasitoids of the Asian Corn Borer Ostrinia furnacalis, Based on ITS2 rDNA Sequence Analysis

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