A molecular epidemiological study of human respiratory syncytial virus in Croatia, 2011–2014

Slović, Anamarija; Ivancic-Jelecki, Jelena; Ljubin‐Sternak, Sunčanica; Galinović, Gordana Mlinarić; Forčić, Dubravko

doi:10.1016/j.meegid.2016.06.036

Cited by 21 publications

(25 citation statements)

References 45 publications

(58 reference statements)

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“…To better understand the epidemiological characteristics of RSV subtypes, RSV-A and RSV-B subtypes were determined based on 623 RSV-positive samples from 13 hospitals. The results showed that RSV-A was the dominant subtype circulating in Guangzhou during 2011-2016, consistent with reports from other regions (Hu et al, 2017;Slovic et al, 2016). The three patients with co-detection were all infants and toddlers and inpatients with ALRI, and none of them died.…”

Section: Discussionsupporting

confidence: 87%

“…Infants and toddlers (<3 years) not in nursery (59.5%) seemed to have the highest risk of RSV infection, and the reason may lie in the decrease in maternal protective antibodies and the increase in exposure opportunities to crowds. The monthly distribution confirmed that RSV was mainly prevalent in winter and spring, consistent with other regions (Bashir et al, 2017;Slovic et al, 2016). The annual distribution of RSV during 2011-2016 in Guangzhou revealed high epidemic years for 2011, 2014, and 2015, with year 2015 as an epidemic peak.…”

Section: Discussionsupporting

confidence: 81%

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Epidemiological characteristics and phylogenic analysis of human respiratory syncytial virus in patients with respiratory infections during 2011–2016 in southern China

Luo

Huang

Zhong

et al. 2020

International Journal of Infectious Diseases

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A B S T R A C TBackground: Human respiratory syncytial virus (RSV) is one of the most important pathogens that cause acute respiratory infections in children and immunocompromised adults. This work was conducted to understand the epidemiological and phylogenetic features of RSV in southern China during 2011-2016. Methods: A total of 16 024 nasopharyngeal swabs were collected from patients with respiratory infections in 14 hospitals, and screened for RSV and seven other respiratory viruses using real-time PCR. Six hundred and twenty-three RSV-positive samples from 13 hospitals were further analyzed for subtypes. G gene sequencing and phylogenetic analysis were performed based on 46 RSV-A and 15 RSV-B strains. Results: RSV was detected in 9.5% of the 16 024 specimens, the highest among the eight respiratory viruses screened. Most of these specimens came from inpatients and children under 3 years of age. The incidence of RSV-A (9.4%) was higher than that of RSV-B (4.4%) in children (<15 years), but not in adults (0.64% vs. 0.58%). A 2-year RSV-A dominance followed by a 1-year RSV-B dominance pattern was found. The co-detection rate of RSV was 25.1%. The main prevalent genotypes were NA1, ON1, and BA9. The prevalent RSV-A genotype in 2011-2012 was NA1, close to Chongqing and Brazil, but a new Hong Kong ON1 genotype was introduced and became the prevalent genotype in Guangzhou in 2014-2015. Deduced amino acid sequence analysis confirmed the ongoing evolution and a high selection pressure of RSV-A and B strains, especially in RSV-A ON1 and NA1 genotypes. Conclusions: This study demonstrated the molecular epidemiological characteristics of RSV in patients with respiratory infections in southern China.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionsupporting

confidence: 81%

Epidemiological characteristics and phylogenic analysis of human respiratory syncytial virus in patients with respiratory infections during 2011–2016 in southern China

Luo

Huang

Zhong

et al. 2020

International Journal of Infectious Diseases

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“…Regarding RSV-B genetic diversity, all belonged to BA genotype, which has already been detected in at least 23 countries [50]. BA9 genotype has predominated in Portugal since 2010 [27,42] as in other countries [10,45,49,51], being detected alongside with BA10 genotype until 2015/16, when BA9 remained the only genotype detected onwards. Since 2001, new genotypes have been added over time by different authors following the proposed criteria by Venter et al [5].…”

Section: Discussionmentioning

confidence: 93%

Epidemiology and genetic variability of respiratory syncytial virus in Portugal, 2014–2018

Sáez-López

Cristóvão

Costa

et al. 2019

Journal of Clinical Virology

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A B S T R A C TIntroduction: Respiratory syncytial virus (RSV) is associated with substantial morbidity and mortality since it is a predominant viral agent causing respiratory tract infections in infants, young children and the elderly. Considering the availability of the RSV vaccines in the coming years, molecular understanding in RSV is necessary. Objective: The objective of the present study was to describe RSV epidemiology and genotype variability in Portugal during the 2014/15-2017/18 period. Material and methods: Epidemiological data and RSV-positive samples from patients with a respiratory infection were collected through the non-sentinel and sentinel influenza surveillance system (ISS). RSV detection, subtyping in A and B, and sequencing of the second hypervariable region (HVR2) of G gene were performed by molecular methods. Phylogenetic trees were generated using the Neighbor-Joining method and p-distance model on MEGA 7.0. Results: RSV prevalence varied between the sentinel (2.5%, 97/3891) and the non-sentinel ISS (20.7%, 3138/ 16779), being higher (P < 0.0001) among children aged < 5 years. Bronchiolitis (62.9%, 183/291) and influenza-like illness (24.6%, 14/57) were associated (P < 0.0001) with RSV laboratory confirmation among children aged < 6 months and adults ≥65 years, respectively. The HVR2 was sequenced for 562 samples. RSV-A (46.4%, 261/562) and RSV-B (53.6%, 301/562) strains clustered mainly to ON1 (89.2%, 233/261) and BA9

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“…By 2006, BA had become the globally dominant RSV-B genotype and almost completely replaced other RSV-B genotypes [55]. BA9 and B10 genotypes were described for the first time in Niigata, Japan in 2007 [29] and were recently the prevalent genotype in many countries [35,[56][57][58][59][60][61][62]. It has been reported that several genotypes within each subgroup can co-circulate in a single population during one epidemic season [30].…”

Section: Discussionmentioning

confidence: 99%

Prevalence and genetic characterisation of respiratory syncytial viruses circulating in Bulgaria during the 2014/15 and 2015/16 winter seasons

Korsun

Angelova

Tzotcheva

et al. 2017

Pathogens and Global Health

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The respiratory syncytial virus (RSV) is a leading cause of acute respiratory illnesses (ARI) in infants and young children. The objectives of this study were to investigate the RSV circulation among children aged <5 years in Bulgaria, to identify the RSV-A and RSV-B genotypes and to perform an amino acid sequence analysis of second hypervariable region (HVR2) of the G gene. During the 2014/15 and 2015/16 winter seasons, nasopharyngeal specimens of 610 children aged <5 years with ARI were tested using Real Time RT-PCR for influenza viruses, RSV, metapneumovirus, parainfluenza viruses, rhinoviruses and adenoviruses. Viral respiratory pathogens were detected in 429 (70%) out of 610 patients examined and RSV was the most frequently identified virus (26%) followed by influenza A(H1N1)pdm09 virus (14%) (p < .05). RSV was the most prevalent pathogen in patients with bronchiolitis (48%) and pneumonia (38%). In the 2014/15 season, RSV-A dominated slightly (53%), while in the next season RSV-B viruses prevailed more strongly (66%). The phylogenetic analysis based on the G gene indicated that all 21 studied RSV-A strains belonged to the ON1 genotype; the vast majority (96%) of the RSV-B strains were classified into BA9 genotype and only one - into BA10 genotype. All Bulgarian RSV-A and RSV-B sequences contained a 72-nt and a 60-nt duplication in the HVR2, respectively. The study showed the leading role of this pathogen as a causative agent of serious respiratory illnesses in early childhood, year-on-year fluctuations in RSV incidence, a shift from RSV-A to RSV-B subgroup dominance and relatively low genetic divergence in the circulating strains.

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A molecular epidemiological study of human respiratory syncytial virus in Croatia, 2011–2014

Cited by 21 publications

References 45 publications

Epidemiological characteristics and phylogenic analysis of human respiratory syncytial virus in patients with respiratory infections during 2011–2016 in southern China

Epidemiological characteristics and phylogenic analysis of human respiratory syncytial virus in patients with respiratory infections during 2011–2016 in southern China

Epidemiology and genetic variability of respiratory syncytial virus in Portugal, 2014–2018

Prevalence and genetic characterisation of respiratory syncytial viruses circulating in Bulgaria during the 2014/15 and 2015/16 winter seasons

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