A Molecular Cytogenetic Study of Chromosome 3 Rearrangements in Small Cell Lung Cancer

Dennis, Thomas R.; Stock, A. Dean

doi:10.1016/s0165-4608(99)00023-0

Cited by 23 publications

(14 citation statements)

References 35 publications

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“…The majority of cell lines analysed here (4/5) did not, however, demonstrate gross inter-chromosomal rearrangements involving chromosome 3. The data presented here does not support the findings of Dennis and Stock, 17 however, the presence of intra-chromosomal rearrangements, which are not detected by M-FISH, involving the region 3q13.2 cannot be ruled out. Analysis of additional primary SCLC specimens will be required to further investigate the role of chromosome 3q in SCLC.…”

Section: Discussioncontrasting

confidence: 99%

“…In general, the chromosomal aberrations present in the primary tumour mirrored those identified in the cell lines with many of the same loci of copy number change detected. The 17 who utilised SKY to analyse the chromosomal rearrangements in a single primary SCLC. This analysis revealed, amongst others, a translocation between chromosome 3 and 12.…”

Section: Discussionmentioning

confidence: 99%

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Chromosomal alterations in small cell lung cancer revealed by multicolour fluorescence in situ hybridization

Ashman

Brigham

Cowen

et al. 2002

Intl Journal of Cancer

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Small cell lung cancer (SCLC) is a major cause of cancer related morbidity and mortality. Karyotypic studies have revealed numerous chromosomal aberrations in most SCLC however, classical G-banding analysis is unable to fully characterise complex marker chromosomes. Recent developments in molecular cytogenetics now allow accurate identification of the chromosomal components of complicated rearrangements. We have applied the technique of multicolour fluorescence in situ hybridization (M-FISH) in combination with comparative genomic hybridization (CGH) to the analysis of 5 SCLC cell lines and 1 primary tumour specimen to characterise the chromosomal abnormalities. CGH analysis identified many similarities between specimens, with frequent DNA copy number decreases on chromosomes 3p, 5q, 10, 16q, 17p and frequent gains on 3q, 1p, 1q and 14q. In contrast, M-FISH analysis revealed a large number of structural abnormalities, with each specimen demonstrating an individual pattern of chromosomal translocations. Forty different translocations were identified with the vast majority (39) being unbalanced. Chromosome 5 was the most frequently rearranged chromosome (9 translocations) followed by chromosomes 2, 10 and 16 (6 translocations each). Further investigation of these frequently involved chromosomes is warranted to establish whether consistent break points are involved in these translocations, causing dysregulation of specific genes that are crucial for tumour progression and secondly to identify the affected genes.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Chromosomal alterations in small cell lung cancer revealed by multicolour fluorescence in situ hybridization

Ashman

Brigham

Cowen

et al. 2002

Intl Journal of Cancer

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“…The FISH findings were generally consistent with routine cytogenetics, which showed a hypertriploid female chromosomal complement with extensive structural and numerical abnormalities observed in nineteen of the twenty cells analyzed: 77-80 < 3n>, XXX [1]. Fluorescence in situ hybridization (FISH) myelodysplastic syndrome (MDS) panel demonstrated a prominent population of hyperdiploid cells with four copies each of chromosomes 5, 7, and 20.…”

Section: Case Reportsupporting

confidence: 71%

“…And while several of the abnormalities observed in this case have been previously reported in cases of small cell lung carcinoma (SCLC), none are specific (1)(2)(3). And while several of the abnormalities observed in this case have been previously reported in cases of small cell lung carcinoma (SCLC), none are specific (1)(2)(3).…”

Section: Case Reportmentioning

confidence: 46%

A Case Of Neuroendocrine Carcinoma Expressing Myeloid Markers By Flow Cytometry And Review Of the Literature

Harrison

Vanderjagt²,

Zhang

2016

Cytometry Part B Clinical

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“…So far, about 40 lung cancer cell lines, and many primary lung carcinoma samples have been analyzed by fluorescence in situ hybridization (FISH) including filter-based M-FISH and spectral karyotyping (SKY), cross-species color banding (RxFISH), and comparative genomic hybridization (CGH) (Dennis et al, 1999;Heppell-Parton et al, 1999;Speicher et al, 2000;Gunawan et al, 2001;Luk et al, 2001;Park et al, 2001;Ashman et al, 2002;Roschke et al, 2003;Berrieman et al, 2004;Grigorova et al, 2005;Tonon et al, 2005;Kume et al, 2007;Shen et al, 2008;Salido et al, 2009). However, no karyotypes of lung carcinoma cell lines induced by radon gas have been analyzed by these molecular cytogenetic approaches.…”

mentioning

confidence: 99%

Characterization of Two Human Lung Adenocarcinoma Cell Lines by Reciprocal Chromosome Painting

Peng¹,

Wang²,

Su³

et al. 2010

Zoological Research

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Lung cancer is a leading cause of cancer death worldwide. Some lung cancer patients correlate with a gas of radon besides smoking. To search for common chromosomal aberrations in lung cancer cell lines established from patients induced by different factors, a combined approach of chromosome sorting, forward and reverse chromosome painting was used to characterize karyotypes of two lung adenocarcinoma cell lines: A549 and GLC-82 with the latter line derived from a patient who has suffered long-term exposure to environmental radon gas pollution. The chromosome painting results revealed that complex chromosomal rearrangements occurred in these two lung adenocarcinoma cell lines. Thirteen and twenty-four abnormal chromosomes were identified in A549 and GLC-82 cell lines, respectively. Almost half of abnormal chromosomes in these two cell lines were formed by non-reciprocal translocations, the others were derived from deletions and duplication/or amplification in some chromosomal regions. Furthermore, two apparently common breakpoints, HSA8q24 and 12q14 were found in these two lung cancer cell lines.

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A Molecular Cytogenetic Study of Chromosome 3 Rearrangements in Small Cell Lung Cancer

Cited by 23 publications

References 35 publications

Chromosomal alterations in small cell lung cancer revealed by multicolour fluorescence in situ hybridization

Chromosomal alterations in small cell lung cancer revealed by multicolour fluorescence in situ hybridization

A Case Of Neuroendocrine Carcinoma Expressing Myeloid Markers By Flow Cytometry And Review Of the Literature

Characterization of Two Human Lung Adenocarcinoma Cell Lines by Reciprocal Chromosome Painting

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