A Molecular and Microscopically Studies of Calicophoron Microbothrium (Paramphistomatidae)

Samn, Alaa Abd-Elaziz

doi:10.12816/0034630

Cited by 3 publications

(3 citation statements)

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“…An aggregation of eosinophils was observed after chemotherapy with Antitrem in the intestinal villi, with the cell membrane preserved, the nuclei necrotic (pyknotic), the granular cell inclusions lysed under the influence of Antitrem, and the cytoplasm of the cells stained orange according to Mallory's triple stain (Samn, 2017). The anthelmintic drug is thought to block the eosinophilic effect on the surrounding host tissues, resulting in their death.…”

Section: Aswan University Journal Of Environmental Studies (Aujes) 2 (2) Pp 115-121 (2021)mentioning

confidence: 99%

“…Paramphistomes are spreading worldwide, particularly in the warmer regions such as: Australia, Africa and India, and primarily, it infects cattle, goats and sheep. Additionally, there are certain species of paramphistomes with medical importance beside economic value, that infect human (Samn, 2017). Paramphistomum cervi is thought-out to be one of the most important species of Paramphistomes.…”

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confidence: 99%

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Changes in the microstructure of the small intestine of sheep infected naturally with Paramphistomum cervi after treatment with Antitrem

Бибик

Abdelhamid

2021

Aswan University Journal of Environmental Studies

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It is clear that the chemical structure of a drug and its process, which are the determinants of the degree of activity an anthelmintic have on the organs and tissues of trematodes, as well as their hosts. This study demonstrates changes in the microstructure of the small intestine of sheep with impulsive Paramphistomiasis after treatment with Antitrem. Seven days after giving "Antitrem (contain oxyclosanide and pyrantela pamoate, OLKAR Release, Russia)" at a dose of 200 mg/kg once perorally, fixed in 70% alcohol and administered according to generally accepted histological methods, the small intestine of the sheep which it was administered to was impulsively attacked by Paramphistomum cervi. Partial atrophy of intestinal mucosa, common dystrophic changes in the epithelium, and destruction of individual goblet cells represented the microstructural changes in the small intestine of infected sheep with Paramphistomum cervi after treatment with Antitrem. Microstructural changes in the small intestine of the sheep are composed of proliferative procedures, a reduction in the content of carbohydrate compounds, and a change in the protein component in the tissues of the organ and stagnation of the secretory product in the cells.

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Section: Aswan University Journal Of Environmental Studies (Aujes) 2 (2) Pp 115-121 (2021)mentioning

confidence: 99%

mentioning

confidence: 99%

Changes in the microstructure of the small intestine of sheep infected naturally with Paramphistomum cervi after treatment with Antitrem

Бибик

Abdelhamid

2021

Aswan University Journal of Environmental Studies

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“…In the last 100 µL of TE buffer was added into the microtubes containing DNA and were stored at -20°C for further processing [30]. The extracted DNA was stored at -20°C and were used for amplification of partial fragment of 18S rRNA sequences (810bp) using specific pair of primer; 18S forward 5'-TCT GTGATGA CTCTGGAT-3' and Reverse 5'-ACC ATTCAAT CGGTAGTA-3' described previously [27].…”

Section: Dna Extractionmentioning

confidence: 99%

Molecular Characterization of Paramphistomum cervi in Buffaloes

Rafiq

Niaz

Zeb

et al. 2020

Acta Scientiae. Vet.

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Background: Paramphistomiasis (Rumen fluke disease) in ruminants is a major health problem, while documented reports on Paramphistomum species are limited in Asian countries. The present study aimed to identify paramphistomoid flukes that infects buffaloes with the goal of characterization of prevalence in Pakistan and its comparison with neighbor countries.Materials, Methods & Results: In 2018, a total of 178 slaughtered buffaloes aged four to six years were examined and flukes were collected from their infected rumen and reticulum using sterilized forceps. After amplification and sequencing of 18S rRNA partial fragment, the generated sequences were edited (810bp) and aligned with the other sequences of Paramphistomum species retrieved from NCBI. A phylogenetic tree was constructed using maximum likelihood method in MEGA X. The 18S rRNA sequence was found 100% similar with Paramphistomum cervi of China and 98% with Paramphistomum epiclitum and other Paramphistomum species of India. The parasitic Pharamphistomum species was identified molecularly as P. cervi.Discussion: Molecular studies provide insight into the biology and phylogenetic relationship among various parasites. These studies are reliable in the genetic-based identification and description of several disease causing agents. The 18S rRNA sequence of P. cervi generated in this study was found closely identical to the P. cervi of the neighbor countries (China and India) which may be due to the similar geographical, environmental conditions and transboundary movement of infected hosts. This is the first nature of study which provides the molecular-based evidence of P. cervi existence in Pakistan and revealed the 18S rRNA as novel molecular marker for the identification and further characterization of Paramphistomum species across Pakistan. The submitted sequence of this study will provide a baseline for further molecular characterization and to compare with other Paramphistoma species from different regions of Pakistan.

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