A molecular analysis of the population of mRNA in bovine spermatozoa

Gilbert, Isabelle; Bissonnette, Nathalie; Boissonneault, Guylain; Vallée, Maud; Robert, Claude

doi:10.1530/rep-06-0292

Cited by 111 publications

(88 citation statements)

References 40 publications

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“…Moreover, a very high abundance of sperm adhesins and most CRISP and Fn-type II mRNAs were found in ejaculated sperm, most of which exhibited weak or no expression in the testes (Song et al 2010(Song et al , 2011. A comparative analysis of the transcripts between bovine spermatids and mature sperm also revealed that although 36.81% were common in both cell types, only 4.5% were specific to mature sperm (Gilbert et al 2007). These data question the hypothesis that the RNA in sperm is leftover from spermiogenesis and suggest that sperm RNAs have other origins.…”

Section: Discussionmentioning

confidence: 98%

“…The presence of coding RNAs in sperms of humans (Lambard et al 2004), pigs (Yang et al 2009), bovines (Gilbert et al 2007, Lalancette et al 2008, mice (Fang et al 2014) and stallions (Das et al 2013) is well established. Even though the functions of the majority of the sperm mRNAs remain enigmatic, it has been proposed that sperm mRNAs may play roles in the regulation of sperm morphology, motility and capacitation and that sperm transcriptional profiles might provide clinical markers for male fertility (Lambard et al 2004, Li et al 2007, Bansal et al 2015.…”

Section: Introductionmentioning

confidence: 99%

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Comparative profiling of small RNAs of pig seminal plasma and ejaculated and epididymal sperm

Cai¹,

Wu²,

Shen³

et al. 2017

Reproduction

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The similarities and differences of small RNAs in seminal plasma, epididymal sperm and ejaculated sperm remain largely undefined. We conducted a systematic comparative analysis of small RNA profiles in pig ejaculated sperm, epididymal sperm and seminal plasma and found that the diversity distribution of small RNA species was generally similar, whereas the abundance of small RNAs is dramatically different across the three libraries; miRNAs and small RNAs derived from rRNA, tRNA, small nuclear RNA, 7SK RNA, NRON RNA and cis-regulatory RNA were enriched in the three libraries, but piRNA was absent. A large population of small RNAs from ejaculated sperm are ejaculated sperm specific, and only 8-30% of small RNAs overlapped with those of epididymal sperm or seminal plasma and a small proportion (5-18%) of small RNAs were shared in the three libraries, suggesting that, in addition to the testes, sperm RNAs may also originate from seminal plasma, epididymis as well as other resources. Most miRNAs were co-distributed but differentially expressed across the three libraries, with epididymal sperm exhibiting the highest abundance, followed by ejaculated sperm and seminal plasma. The prediction of target genes of the top 10 highly expressed miRNAs across the three libraries revealed that these miRNAs may be involved in spermatogenesis, zygote development and the interaction between the environment and animals. Our study provides the first description of the similarities and differences of small RNA profiles in ejaculated sperm, epididymal sperm and seminal plasma and indicates that sperm RNA may have origins other than the testes.Reproduction (2017) 153 [785][786][787][788][789][790][791][792][793][794][795][796]

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Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Comparative profiling of small RNAs of pig seminal plasma and ejaculated and epididymal sperm

Cai¹,

Wu²,

Shen³

et al. 2017

Reproduction

View full text Add to dashboard Cite

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“…For example, Jasko et al (1990a) and findings provided a better concept of the biological importance of sperm RNAs, allowing the identification of biomarkers of stallion fertility. The sperm transcriptome has been studied in humans (Ostermeier et al 2002), boars (Kempisty et al 2008;Yang et al 2009) and bulls (Gilbert et al 2007;Bissonnette et al 2009;Feugang et al 2010), and the utility of sperm RNA as a marker of infertility has been explored (Miller 2000;Steger 2001), whereby differences in transcript levels in spermatozoa with different motilities (Bissonnette et al 2009;Lambard et al 2004), as well as between normal and abnormal sperm samples (Platts et al 2007;Steger et al 2003), have been reported. Krawetz (2005) demonstrated that some of the sperm RNAs can be delivered to the oocyte during fertilisation.…”

Section: Introductionmentioning

confidence: 99%

Implication of transcriptome profiling of spermatozoa for stallion fertility

Suliman

Becker

Wimmers

2018

Reprod. Fertil. Dev.

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Abstract. Poor fertility of breeding stallions is a recognised problem in the equine industry. The aim of the present study was to detect molecular pathways using two groups of stallions that differed in pregnancy rates as well as in the proportion of normal and motile spermatozoa. RNA was isolated from spermatozoa of each stallion and microarray data were analysed to obtain a list of genes for which transcript abundance differed between the groups (P #0.05, fold change $1.2). In all, there were 437 differentially expressed (DE) genes between the two groups (P # 0.05, fold change $1.2). Next, the DE genes were analysed using Database for Annotation, Visualisation, and Integrated Discovery (DAVID). Finally, ingenuity pathways analysis (IPA) was used to identify top biological functions and significant canonical pathways associated with the DE genes. Analysis using the DAVID database showed significant enrichment in the gene ontology (GO) term 'RNA binding' (P ¼ 0.05) and in the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway cytokinecytokine receptor interaction (P ¼ 0.02). Furthermore, IPA analysis showed interconnected biological functions and canonical pathways involved in the regulation of spermatogenesis and male fertility. In addition, significantly enriched metabolic pathways were identified. In conclusion, the present study has identified, for the first time, molecular processes in stallion spermatozoa that could be associated with stallion fertility.

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“…16,17 A previous study, aiming to determine the functional role of mRNA transcripts in bull spermatozoa, supports the result that sperm RNA profiling is suitable for molecular diagnosis of male gamete quality. 18 There are some studies researching the levels of different transcripts of mRNA encoding molecules containing nuclear condensation, capasity and sperm function of spermatozoa. 19,20 In these studies, it has been submitted that the quality of sperm can be related with its mRNA distribution.…”

Section: Discussionmentioning

confidence: 99%

NRF2 mRNA Expression in Ejaculate Spermatozoa from Men with Asthenozoospermia and Oligoasthenozoospermia

Kara¹,

Taşdemir²,

Tunçez³

et al. 2017

TJRMS

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A AB BS S T TR RA AC CT TO Ob bj je ec ct ti iv ve es s: : We aimed to determine whether the level of mRNA expression of antioxidant gene nuclear factor erythroid 2-related factor 2 (NRF2) in spermatozoa is associated with sperm function and also the seminal plasma superoxide dismutase (SOD) enzyme activity was to investigate in asthenozoospermia and oligoasthenozoospermia cases. M Ma at te er ri ia al l a an nd d M Me et th ho od ds s: : In this study asthenozoospermic and oligoasthenozoospermic 41 patients and normozoospermic 48 healthy individuals were included. Quantitative real-time reverse transcriptase polymerase chain reaction was used for detecting level of NRF2 mRNA expression in ejaculated spermatozoa and colorimetric method was also used to evaluate seminal plasma SOD activity. R Re es su ul lt ts s: : Results revealed that both the level of NRF2 mRNA expression and the seminal plasma SOD activity levels have no statistically significant difference between the two study groups (respectively p=0.633, p=0.502). A significant correlation was not observed between the level of NRF2 mRNA expression and specific sperm function parameters (concentration, vitality, immotility, progressive motility, non-progressive motility, abnormal morphology) and also seminal plazma SOD activity (respectively; all p>0.05, p=0.553). Additionally there was no significant correlation between seminal plasma SOD activity and specific sperm function parameters (all p>0.05). C Co on nc cl lu us si io on n: : These data indicated that NRF2 mRNA expression level and seminal plasma SOD activity did not show any significant difference in men with low sperm motility and that these factors were not related to specific sperm function parameters. As a result, it is thought that the NRF2 gene cannot be sufficient for using as a marker to determine the male infertility. In addition, this study showed that the SOD activity is not a sufficient marker to predict sperm fertilization potential.K Ke ey y W Wo or rd ds s: : NFE2L2 protein, human; superoxide dismutase; spermatogenesis; sperm motility Ö ÖZ ZE ET T G Gi ir ri iş ş: : Asthenozoospermik ve oligoasthenozoospermik olguların spermatozoalarında antioksidan gen olan transkripsiyon faktörü nükleer faktör eritroid 2 ilişkili faktör 2 (NRF2)'nin mRNA ekspresyon düzeyinin sperm fonksiyonları ile olan ilişkisi ve ayrıca seminal plazma süperoksit dismutaz (SOD) enzim aktivitesinin araştırılması amaçlandı. G Ge er re eç ç v ve e Y Yö ön nt te em ml le er r: : Bu çalışmaya, 41 asthenozoospermik ve oligoasthenozoospermik olgudan oluşan hasta grubu ve 48 sağlıklı bireyden oluşan normozoospermik kontrol grubu dahil edildi. Olguların ejekulat spermatozoalarında NRF2 mRNA ekspresyon düzeyini belirlemek için kantitatif real-time reverse transkriptaz polimeraz zincir reaksiyonu ve seminal plazma SOD aktivitesini değer-lendirmek için ise kolorimetrik yöntem kullanıldı. B Bu ul lg gu ul la ar r: : Araştırmamız sonucunda, hasta grubunun hem NRF2 mRNA ekspresyon düzeyi hem de seminal plazma SOD aktivitesi kon...

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A molecular analysis of the population of mRNA in bovine spermatozoa

Cited by 111 publications

References 40 publications

Comparative profiling of small RNAs of pig seminal plasma and ejaculated and epididymal sperm

Comparative profiling of small RNAs of pig seminal plasma and ejaculated and epididymal sperm

Implication of transcriptome profiling of spermatozoa for stallion fertility

NRF2 mRNA Expression in Ejaculate Spermatozoa from Men with Asthenozoospermia and Oligoasthenozoospermia

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