A Moldy Application of MALDI: MALDI-ToF Mass Spectrometry for Fungal Identification

Patel, Robin

doi:10.3390/jof5010004

Cited by 112 publications

(80 citation statements)

References 76 publications

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“…The rate of correct identification obtained for Aspergillus with IDFP medium can be considered as good regarding rates already described in the literature that are very variable ranging from 0% to 100%. 16,19,22,[24][25][26] For non-Aspergillus species, a rate of 73% of correct identification was obtained after 24 hours following complete formic acid acetonitrile extraction. So also in this case, protein extraction is encouraged to obtain optimal results.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the new Id‐Fungi plates from Conidia for MALDI‐TOF MS identification of filamentous fungi and comparison with conventional methods as identification tool for dermatophytes from nails, hair and skin samples

Sacheli¹,

Henri²,

Seidel

et al. 2020

Mycoses

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Objectives We first compare the efficiency of mould/dermatophyte identification by MALDI‐TOF MS using a new medium called Id‐Fungi plates (IDFP) from Conidia® and two different databases. For the second purpose, we evaluated a new version of the medium supplemented with cycloheximide, Id‐Fungi plates Plus (IDFPC) for the direct inoculation of nails, hair and skin samples and compared the efficiency of MALDI‐TOF MS identification of dermatophytes to classical methods based on culture and microscopy. Methods A total of 71 strains have been cultured IDFP and Sabouraud gentamicin plates (SGC2) and were identified by MALDI‐TOF MS. For the evaluation of the combination IDFPC/ MALDI‐TOF MS as a method of identification for dermatophytes, 428 samples of hair nails and skin were cultivated in parallel on IDFPC and Sabouraud + cycloheximide medium (SAB‐ACTI). Results For Aspergillus sp and non‐Aspergillus moulds, the best performances were obtained on IDFP after maximum 48‐h growth, following protein extraction. For dermatophytes, the best condition was using the IDFP at 72 hours, after extended direct deposit. Regarding the direct inoculation of nails, hair skin on IDFPC, 129/428 (30.1%) showed a positive culture against 150/428 (35%) on SAB‐ACTI medium. Among the 129 positive strains, the identification by MALDI‐TOF MS was correct for 92/129 (71.4%). Conclusion The IDFP allows the generation of better spectra by MALDI‐TOF MS compared to SGC2. It facilitates sampling and deposit. Regarding the use of IDFPC, this medium seems less sensitive than SAB‐ACTI but among positive strains, the rate of correct identification by MALDI‐TOF MS is satisfactory.

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Section: Discussionmentioning

confidence: 99%

Evaluation of the new Id‐Fungi plates from Conidia for MALDI‐TOF MS identification of filamentous fungi and comparison with conventional methods as identification tool for dermatophytes from nails, hair and skin samples

Sacheli¹,

Henri²,

Seidel

et al. 2020

Mycoses

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“…In diagnostic facilities, matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) is being increasingly used for the identification of filamentous fungi. In the last few years, several studies have shown the potential of this method for a fast identification of the Mucorales [108][109][110]. Using the VITEK®MS v3.0 system, a species identification rate of 86% was achieved for 118 mucoralean isolates [111].…”

Section: Identification and Detectionmentioning

confidence: 99%

Updates on the Taxonomy of Mucorales with an Emphasis on Clinically Important Taxa

Walther

Wagner

Kurzai

2019

JoF

141

125

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Fungi of the order Mucorales colonize all kinds of wet, organic materials and represent a permanent part of the human environment. They are economically important as fermenting agents of soybean products and producers of enzymes, but also as plant parasites and spoilage organisms. Several taxa cause life-threatening infections, predominantly in patients with impaired immunity. The order Mucorales has now been assigned to the phylum Mucoromycota and is comprised of 261 species in 55 genera. Of these accepted species, 38 have been reported to cause infections in humans, as a clinical entity known as mucormycosis. Due to molecular phylogenetic studies, the taxonomy of the order has changed widely during the last years. Characteristics such as homothallism, the shape of the suspensors, or the formation of sporangiola are shown to be not taxonomically relevant. Several genera including Absidia, Backusella, Circinella, Mucor, and Rhizomucor have been amended and their revisions are summarized in this review. Medically important species that have been affected by recent changes include Lichtheimia corymbifera, Mucor circinelloides, and Rhizopus microsporus. The species concept of Rhizopus arrhizus (syn. R. oryzae) is still a matter of debate. Currently, species identification of the Mucorales is best performed by sequencing of the internal transcribed spacer (ITS) region. Ecologically, the Mucorales represent a diverse group but for the majority of taxa, the ecological role and the geographic distribution remain unknown. Understanding the biology of these opportunistic fungal pathogens is a prerequisite for the prevention of infections, and, consequently, studies on the ecology of the Mucorales are urgently needed.

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“…11,12 MALDI-TOF MS is used in the analysis of portions of mycelium and/or conidia from fungal cultures, and is considered to be an accurate and rapid identification method. 13,14 The aim of the present study was to evaluate the application of MALDI-TOF-MS for the identification of and discrimination between A flavus and A oryzae in clinical and environmental samples and compare its diagnostic power with that of β-tubulin gene sequencing. Accumulating evidence suggests that MALDI-TOF MS is a promising approach to rapid and accurate identification of different clinically relevant pathogens, 10,23,24 which was also successfully applied to discriminate among Aspergillus species.…”

Section: Dna Relatedness Among a Flavus A Oryzae A Parasiticus And mentioning

confidence: 99%

“…MALDI‐TOF MS is used in the analysis of portions of mycelium and/or conidia from fungal cultures, and is considered to be an accurate and rapid identification method . The aim of the present study was to evaluate the application of MALDI‐TOF‐MS for the identification of and discrimination between A flavus and A oryzae in clinical and environmental samples and compare its diagnostic power with that of β‐tubulin gene sequencing.…”

Section: Introductionmentioning

confidence: 99%

Discrimination of Aspergillus flavus from Aspergillus oryzae by matrix‐assisted laser desorption/ionisation time‐of‐flight (MALDI‐TOF) mass spectrometry

Hedayati

Armaki

Zarrinfar

et al. 2019

Mycoses

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Summary Background Aspergillus flavus is a major cause of severe non‐invasive fungal infections in the Middle Eastern countries. However, it is difficult to distinguish A flavus from A oryzae. Objectives To assess the potential of matrix‐assisted laser desorption/ionisation time‐of‐flight mass spectrometry (MALDI‐TOF MS) in discriminating between A flavus and A oryzae and compare it with β‐tubulin gene sequencing. Methods We used the Bruker Daltonik MALDI‐TOF MS system to analyse 200 clinical and environmental A flavus isolates and one A pseudonomius and one A alliaceus (Aspergillus section Flavi) isolate a priori identified as such by sequencing of the β‐tubulin gene. Results All 200 A flavus isolates were identified at the genus level and 176 (88%) at the species levels by MALDI‐TOF MS based on the spectral log‐scores (≥2.0 and 1.7‐1.99, respectively); among them, only 18 (10.2%) were confirmed as A flavus, whereas 35 (19.9%) were identified as A oryzae and 123 (69.9%) as A flavus/A oryzae. Aspergillus pseudonomius and A alliaceus were misidentified as A flavus and A parasiticus with log‐score values of 1.39 and 1.09, respectively. Conclusions The results indicate that the commercially available Bruker Daltonik MALDI‐TOF MS score database cannot separate A flavus and A oryzae species. We also showed that establishment of an in‐house library is a useful tool to discriminate closely related Aspergillus species, including A flavus and A oryzae.

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A Moldy Application of MALDI: MALDI-ToF Mass Spectrometry for Fungal Identification

Cited by 112 publications

References 76 publications

Evaluation of the new Id‐Fungi plates from Conidia for MALDI‐TOF MS identification of filamentous fungi and comparison with conventional methods as identification tool for dermatophytes from nails, hair and skin samples

Evaluation of the new Id‐Fungi plates from Conidia for MALDI‐TOF MS identification of filamentous fungi and comparison with conventional methods as identification tool for dermatophytes from nails, hair and skin samples

Updates on the Taxonomy of Mucorales with an Emphasis on Clinically Important Taxa

Discrimination of Aspergillus flavus from Aspergillus oryzae by matrix‐assisted laser desorption/ionisation time‐of‐flight (MALDI‐TOF) mass spectrometry

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