A Modular Fluorescent Probe for Viscosity and Polarity Sensing in DNA Hybrid Mesostructures

Ludwanowski, Simon; Samanta, Avik; Loescher, Sebastian; Barner‐Kowollik, Christopher; Walther, Andreas

doi:10.1002/advs.202003740

Cited by 41 publications

(58 citation statements)

References 39 publications

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“…As in Equation ( 4), the anisotropy is sensitive to the liquid temperature, the viscosity, and the lifetime. In an isothermal system, the anisotropy is utilized as a viscous indicator [33,34]. Here, we intend to utilize the temperature dependence of the anisotropy to measure the liquid temperature field in microfluidics.…”

Section: Resultsmentioning

confidence: 99%

Fluorescence Anisotropy as a Temperature-Sensing Molecular Probe Using Fluorescein

2021

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Fluorescence anisotropy, a technique to study the folding state of proteins or affinity of ligands, is used in this present work as a temperature sensor, to measure the microfluidic temperature field, by adding fluorophore in the liquid. Fluorescein was used as a temperature-sensing probe, while glycerol–aq. ammonia solution was used as a working fluid. Fluorescence anisotropy of fluorescein was measured by varying various parameters. Apart from this, a comparison of fluorescence anisotropy and fluorescence intensity is also performed to demonstrate the validity of anisotropy to be applied in a microfluidic field with non-uniform liquid thickness. Viscosity dependence and temperature dependence on the anisotropy are also clarified; the results indicate an appropriate selection of relation between molecule size and viscosity is important to obtain a large temperature coefficient in anisotropy. Furthermore, a practical calibration procedure of the apparatus constant is proposed. In addition, the potential of temperature imaging is confirmed by the measurement of temperature distribution under focused laser heating.

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Section: Resultsmentioning

confidence: 99%

Fluorescence Anisotropy as a Temperature-Sensing Molecular Probe Using Fluorescein

2021

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“…Viscosity is a key indicator of biological microenvironments related to various physiological and pathological processes. Monitoring the levels of viscosity can provide critical information for related diseases 116 , 117 . The developed viscosity-activatable probes show various strategies, including interactions between the targeting group and the skeleton (probe 30), conjugation effect of D-π-A structure (probe 31), fluorescent molecular rotors based on fluorescence lifetime imaging (probe 32) etc.…”

Section: Microenvironmentmentioning

confidence: 99%

Activatable NIR-II organic fluorescent probes for bioimaging

Zhang¹,

Li²,

Ma³

et al. 2022

Theranostics

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NIR-II imaging is developed rapidly for noninvasive deep tissue inspection with high spatio-temporal resolution, taking advantage of diminished autofluorescence and light attenuation. Activatable NIR‐II fluorescence probes are widely developed to report pathological changes with accurate targeting, among which organic fluorescent probes achieve significant progress. Furthermore, the activatable NIR‐II fluorescent probes exhibited appealing characteristics like tunable physicochemical and optical properties, easy processability, and excellent biocompatibility. In the present review, we highlight the advances of activatable NIR-II fluorescence probes in design, synthesis and applications for imaging pathological changes like reactive oxygen species (ROS), reactive nitrogen species (RNS), reactive sulfur species (RSS), pH, hypoxia, viscosity as well as abnormally expressed enzymes. This non-invasive optical imaging modality shows a promising prospect in targeting the pathological site and is envisioned for potential clinical translation.

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“…[74] Finally, it is worth mentioning that the use of multiple emissions from the same emitter allows one to measure different parameters (e.g., temperature, pH, ROS) in the same assay using a single sensor. [83][84][85][86][87][88][89] In this same line, distinct compounds (e.g., different ROS) can be detected using a fluorescent sensor that emit at a different color in the presence of different analytes. [90][91][92] Moreover, the emitter can be designed to present emission bands in the visible and infrared ranges to be used for both in vitro and in vivo fluorescence molecular detection.…”

Section: Sensing Techniquesmentioning

confidence: 99%

Multichannel Fluorescence Microscopy: Advantages of Going beyond a Single Emission

Rodríguez‐Sevilla

Thompson

Jaque

2022

Advanced NanoBiomed Research

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Fluorescent microscopy has enabled the study of intracellular processes and revealed the most intricate details of the subcellular structure. This has benefitted not only the basic biological science, but also has had an impact in numerous biomedical applications. Basic fluorescent sensing techniques use the change in the absolute emission of a fluorescent sensor. This entails some disadvantages as the signal might be influenced by factors not directly related to the process under study (e.g., fluctuations in the excitation source). To overcome these drawbacks, one can use multiple emissions of a single or various fluorophores. There are numerous examples of multichannel fluorescence microscopy techniques that have given rise to numerous ratiometric methods and multiplexing assays. Herein, how the use of multiple emission channels has impacted fluorescence microscopy in terms of speed, sensitivity, and resolution is reviewed. Using recent examples, how the easy implementation of multichannel detection can overcome current limitations of the main used fluorescence techniques and promote the development of novel microscopy methods is shown.

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A Modular Fluorescent Probe for Viscosity and Polarity Sensing in DNA Hybrid Mesostructures

Cited by 41 publications

References 39 publications

Fluorescence Anisotropy as a Temperature-Sensing Molecular Probe Using Fluorescein

Fluorescence Anisotropy as a Temperature-Sensing Molecular Probe Using Fluorescein

Activatable NIR-II organic fluorescent probes for bioimaging

Multichannel Fluorescence Microscopy: Advantages of Going beyond a Single Emission

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