A modular BioMEMS platform for new procedures and experiments in tissue engineering

Stubenrauch, Mike; Fröber, Ulrike; Voges, Danja; Schilling, Cornelius; Hoffmann, Martin; Witte, Hartmut

doi:10.1088/0960-1317/19/7/074013

Cited by 7 publications

(7 citation statements)

References 6 publications

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“…First, the results using MC3T3‐E1 cells seeded into the cultivation chamber by flushing in (Fig. 3A) show good viability, but spread uncontrollably all over the system's inner surfaces 6. In order to approach 3‐D cell cultivation, primary bovine chondrocytes were seeded on a scaffold (2 mm×2 mm 0.5 mm).…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Integration of 3‐D cell cultures in fluidic microsystems for biological screenings

Witte¹,

Stubenrauch²,

Fröber³

et al. 2011

Engineering in Life Sciences

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A life support system for the cultivation of adherent 2‐D and scaffold‐based 3‐D cell cultures in a microfluidic device, a Bio‐Micro‐Electro‐Mechanical System (BioMEMS) is presented. The miniaturization level and system set‐up allow incubator‐independent operation modes and long‐term experiments with real‐time microscope observation. A dedicated seeding procedure for adherent cells into the microstructures is one key issue of the BioMEMS developed. Several seeding methods for the cells were evaluated. Biocompatibility of all materials, surfaces and methods could be demonstrated. First experiments with several cell types show the feasibility of the approach employing standard laboratory protocols. At present, the modular design and set‐up offer a broad application spectrum as well as its future extension to e.g. cultivation of other cell types, coupled cultivation chambers and the implementation of other manipulation or analysis components.

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Section: Resultsmentioning

confidence: 99%

“…Taking all these aspects into account, the general technology workflow has to be established long before the final design of the structures. Simple prototypes of our BioMEMS were fabricated, tested and published 6 in the past. A simplified, exemplary workflow for such cell‐cultivation BioMEMS is shown in Figure 1.…”

Section: Methodsmentioning

confidence: 99%

Integration of 3‐D cell cultures in fluidic microsystems for biological screenings

Witte¹,

Stubenrauch²,

Fröber³

et al. 2011

Engineering in Life Sciences

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“…The overall dimensions (2 Â 2 Â 0.25 mm 3 , 1875 unit cells) of the cubic lattice scaffolds were adapted to the geometry of a flow-through chamber inside a microfluidic BioMEM, which was constructed for 4D analysis (x, y, z and time) of 3D cell cultures using TPLSM. [91,92] Even larger scaffolds with a volume of 8 Â 8 Â 2 mm 3 were produced by scaling up the unit cell dimensions to 500 mm (Fig. 6).…”

Section: Synthesis and Characterization Of Photopolymersmentioning

confidence: 99%

Two‐Photon Polymerization of Biocompatible Photopolymers for Microstructured 3D Biointerfaces

et al. 2011

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Three‐dimensional microstructured scaffolds provide a means for cells to be cultured in vitro in a way that resembles natural conditions more closely than flat tissue culture polystyrene. In the presented work, two‐photon polymerization (2PP) is applied as a tool for the engineering of high‐resolution 3D scaffold structures with a well defined microarchitecture made of biocompatible photo resins. 2PP is a novel photolithographic technique using femtosecond laser pulses which enables free 3D microstructuring of liquid photo resins due to the relationship of the axial and lateral spatial confinement of the photoreaction to the focal volume of a focused laser beam. A set of photo resins were tested with regard to 2PP processability and three different classes of methacrylated photopolymerizable monomers (methacrylated oligolactones, urethane dimethacrylate, poly(ethylene glycol diacrylate)) were found to be efficient 2PP materials. 3D microstructures based on computer models were produced and tested for biocompatibility. The initial cell adhesion and the viability of bovine chondrocytes on the polymeric scaffolds were evaluated morphologically by confocal laser scanning microscopy (CLSM) after three‐day culture on 2PP derived microstructures. 2PP derived scaffolds were fabricated in different sizes and geometries, starting from the 100 µm‐range reaching out to the cm‐range showing the actual possibilities to produce large volume scaffolds even for implantation purposes.

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“…The front side of the substrate is covered with a glass wafer (Borofloat ® ) by anodic bonding to allow optical access and to close to the microfluidic chambers and channels. As said before, additional silicon components can be plugged into the microsystem from the backside via Velcro ® -like interface areas, see also [1]. They have got two functionalities.…”

Section: Biomemsmentioning

confidence: 99%

BioMEMS for Processing and Testing of Hydrogel-Based Bio-Interfaces

Stubenrauch¹,

Fischer²,

Fröber³

et al. 2012

Biomedical Engineering / Biomedizinische Technik

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A BioMEMS platform for development, processing and testing of hydrogels is presented. It is based on standard silicon micromachining including smart assembly and interconnection techniques. Hydrogel materials can be brought into the device by several methods. Material tests and functionalizations of the hydrogels can take place in the system as well as cell cultivation for biotests. First results are promising for easy and parallel testing of several materials / samples and for further biomedical applications.

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A modular BioMEMS platform for new procedures and experiments in tissue engineering

Cited by 7 publications

References 6 publications

Integration of 3‐D cell cultures in fluidic microsystems for biological screenings

Integration of 3‐D cell cultures in fluidic microsystems for biological screenings

Two‐Photon Polymerization of Biocompatible Photopolymers for Microstructured 3D Biointerfaces

BioMEMS for Processing and Testing of Hydrogel-Based Bio-Interfaces

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