A modular and compact portable mini-endstation for high-precision, high-speed fixed target serial crystallography at FEL and synchrotron sources

Sherrell, D.A.; Foster, Andrew J.; Hudson, L.; Nutter, Brian; O'Hea, J.; Nelson, S.; Paré-Labrosse, Olivier; Oghbaey, Saeed; Miller, R. J. Dwayne; Owen, Robin L.

doi:10.1107/s1600577515016938

Cited by 63 publications

(70 citation statements)

References 22 publications

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“…So far, such experiments have been performed in step-scanning mode, rendering them less efficient than other sample delivery methods due to the relatively long data collection times 34,38 . Another obstacle for fixed-target experiments is the relatively high background scattering level, mainly caused by air scattering from the direct beam.…”

Section: Introductionmentioning

confidence: 99%

High-speed fixed-target serial virus crystallography

et al. 2017

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21We have developed a method for serial X-ray crystallography at X-ray free electron lasers (XFELs), 22 which allows for full use of the current 120 Hz repetition rate of the Linear Coherent Light Source 23 (LCLS). Using a micro-patterned silicon chip in combination with the high-speed Roadrunner 24 goniometer for sample delivery we were able to determine the crystal structures of a picornavirus, 25 bovine enterovirus 2 (BEV2), and the cytoplasmic polyhedrosis virus type 18 polyhedrin. Total data 26 collection times were less than 14 and 10 minutes, respectively. Our method requires only micrograms 27 of sample and will therefore broaden the applicability of serial femtosecond crystallography to 28 challenging projects for which only limited amounts of samples are available. By synchronizing the 29 sample exchange to the XFEL repetition rate it further allows for the most efficient use of the limited 30 beamtime available at XFELs and a significant increase in sample throughput at these facilities. 31 32 Introduction 33

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Section: Introductionmentioning

confidence: 99%

High-speed fixed-target serial virus crystallography

et al. 2017

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“…A fixed target method is now capable of delivering crystals at the repetition rates of the existing XFEL sources [50] . Microfluidic flow cells would be useful for precious protein samples [51,52] .…”

Section: Discussionmentioning

confidence: 99%

Fluid sample injectors for x-ray free electron laser at SACLA

Tono

2017

High Pow Laser Sci Eng

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This paper provides a review on sample injectors which are provided at SPring-8 Angstrom Compact free electron LAser (SACLA) for conducting serial measurement in a 'diffract-before-destroy' scheme using an x-ray free electron laser (XFEL). Versatile experimental platforms at SACLA are able to accept various types of injectors, among which liquidjet, droplet and viscous carrier injectors are frequently utilized. These injectors produce different forms of fluid targets such as a liquid filament with a diameter in the order of micrometer, micro-droplet synchronized to XFEL pulses, and slowly flowing column of highly viscous fluid with a rate below 1 µL min −1 . Characteristics and applications of the injectors are described.

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“…All of the above data were recorded using the CSPAD detector. Diffraction patterns from myoglobin crystals crystallized in space group P2 1 2 1 2 1 provided an example of an orthorhombic space group and were recorded using a Rayonix MX170-HS detector at the XPP endstation using an alternative siliconchip delivery system (Sherrell et al, 2015;Zarrine-Afsar et al, 2012;Mueller et al, 2015), which has been shown to support structure solution (Oghbaey et al, 2016).…”

Section: Data Acquisition For Various Protein Crystalsmentioning

confidence: 99%