A Modified Silver Method for Demonstrating Developing Nervous Tissue in Culture

Abstract

Dissociated explants of 8-day-old embryonic chick cerebrum were cultured for up to 18 days. By the beginning of the 2nd week and thereafter, primary cultures of neurons exhibited characteristic differentiated morphology with an interconnecting neurofibrillary network that became increasingly ramified. Neurons in bipolar, tripolar or multipolar form could be demonstrated positively using a short modified silver impregnation method with potassium ferrocyanide.

Neurotrophic effects of GABA in cultures of embryonic chick brain and retina

“…The modification of Ogawa's (1979) silver impregnation technique was used to identify the purity of the neuronal cultures as previously described (Spoerri et al, 1985). Neuronal cells grown on plastic dishes for 2, 4, and 7 days were rinsed with PBS kept at 37"C, fixed in methanol for 5 min, and dried at 37°C.…”

A Silver Impregnation Method for Embedded Central Nervous Tissue of the Rat

“…These procedures were suitable for frozen sections. In a recent investigation, a modified silver method with potassium ferrocyanide for developing neurons in culture has been described [Spoerri et al, 1985]. Presently, the authors report on a similar but somewhat modified method for staining paraffin-or paraplast-embedded tissue from the central nervous system of the rat.…”