A modified porous silicon microparticle promotes mucosal delivery of SARS-CoV-2 antigen and induction of potent and durable systemic and mucosal T helper 1 skewed protective immunity

Adam, Awadalkareem; Shi, Qing; Wang, Binbin; Zou, Jing; Mai, Junhua; Osman, Samantha R.; Wu, Wenzhe; Xie, Xuping; Aguilar, Patricia V.; Bao, Xiaoyong; Shi, Pei–Yong; Shen, Haifa; Wang, Tian

doi:10.1101/2021.11.22.469576

Cited by 3 publications

(2 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The intranasal vaccine stimulated both local mucosal and systemic immunity [ 242 ]. Also, a modified intranasal porous silicon microparticle (mPSM)-adjuvanted SARS-CoV-2 RBD vaccine was developed [ 243 ]. Boost vaccinations with intranasal mPSM-RBD vaccine diminished lung inflammation and viral loads following Delta variant infection [ 243 ].…”

Section: Needle-free Particulate Delivery Systems Of Covid-19 Vaccinementioning

confidence: 99%

“…Also, a modified intranasal porous silicon microparticle (mPSM)-adjuvanted SARS-CoV-2 RBD vaccine was developed [ 243 ]. Boost vaccinations with intranasal mPSM-RBD vaccine diminished lung inflammation and viral loads following Delta variant infection [ 243 ]. It stimulated SARS-CoV-2 specific IgA responses, lung resident memory T and B cells, along with potent systemic immunity [ 243 ].…”

Section: Needle-free Particulate Delivery Systems Of Covid-19 Vaccinementioning

confidence: 99%

See 1 more Smart Citation

Towards novel nano-based vaccine platforms for SARS-CoV-2 and its variants of concern: Advances, challenges and limitations

Helmy

El-Morsi

Helmy

et al. 2022

Journal of Drug Delivery Science and Technology

View full text Add to dashboard Cite

Section: Needle-free Particulate Delivery Systems Of Covid-19 Vaccinementioning

confidence: 99%

Section: Needle-free Particulate Delivery Systems Of Covid-19 Vaccinementioning

confidence: 99%

Towards novel nano-based vaccine platforms for SARS-CoV-2 and its variants of concern: Advances, challenges and limitations

Helmy

El-Morsi

Helmy

et al. 2022

Journal of Drug Delivery Science and Technology

View full text Add to dashboard Cite

Understanding 3D genome organization by multidisciplinary methods

Jerković

Cavalli

2021

Nat Rev Mol Cell Biol

242

172

View full text Add to dashboard Cite

Understanding how chromatin is folded in the nucleus is fundamental to understanding its function. Although 3D genome organization has been historically difficult to study owing to lack of relevant methodologies, major technological breakthroughs in genome-wide mapping of chromatin contacts and advances in imaging technologies in the 21 st century considerably improved our understanding of chromosome conformation and nuclear architecture.In this Review, we discuss methods of 3D genome organization analysis, including sequencing-based techniques, such as Hi-C and its derivatives, micro-C, DamID and others; microscopy-based techniques, such as super-resolution imaging coupled with fluorescent in situ hybridization (FISH), multiplex FISH, in situ genome sequencing and live microscopy methods; and computational and modeling approaches. We describe the most commonly used techniques and their contribution to our current knowledge of nuclear architecture and, finally, we provide a perspective on up-and-coming methods that open possibilities for future major discoveries.Simultaneously with the development of the C-based techniques, ligation-independent techniques were invented to assay not only chromosome conformation in general, but also the nuclear position of chromatin contacts (tyramide signal amplification (TSA), DNA adenine methyltransferase identification (DamID), split-pool recognition of interactions by tag extension (SPRITE)) and multi-way contacts (SPRITE and genome architecture mapping (GAM)), which are not assayed effectively using ligation-based techniques [34][35][36][37][38][39][40][41][42] . Finally, the recent advancement of superresolution microscopy and imaging techniques allowed us to investigate chromatin conformation of single cells at extremely high resolution and at a higher throughput than ever before 12,14,15,[43][44][45][46][47][48] . In addition to improvements in spatial resolution, live-imaging in combination with genome-engineering using CRISPR-Cas9 systems facilitated and improved the study of chromatin-contact dynamics [49][50][51] .Owing to these methodological and technological advancements, it is not surprising that the past decade has provided major revelations in 3D genome organization and function. Most notably is the finding that chromosomes in interphase predominantly fold into two compartments, A and B, which respectively consist of predominantly gene-active and gene-inactive regions 27 (Figure 1). Furthermore, parts of compartments, from the same or different chromosomes, can come together and create hubs, which are connected by multiple chromatin interactions, thereby sharing a common function (for example, gene repression) and coalescing around different nuclear bodies such as nuclear speckles 35,37,52 . On a scale below the compartments, chromatin interactions were found to be enriched within domains of 100 kb to 1 Mb in length termed topologically associating domains (TADs); these partially insulated domains are subdivided into smaller chromatin nanodomains (CNDs) 43,53-57 ...

show abstract

Structural insights into nuclear transcription by eukaryotic DNA-dependent RNA polymerases

Girbig

Misiaszek

Müller

2022

Nat Rev Mol Cell Biol

View full text Add to dashboard Cite

A modified porous silicon microparticle promotes mucosal delivery of SARS-CoV-2 antigen and induction of potent and durable systemic and mucosal T helper 1 skewed protective immunity

Cited by 3 publications

References 50 publications

Towards novel nano-based vaccine platforms for SARS-CoV-2 and its variants of concern: Advances, challenges and limitations

Towards novel nano-based vaccine platforms for SARS-CoV-2 and its variants of concern: Advances, challenges and limitations

Understanding 3D genome organization by multidisciplinary methods

Structural insights into nuclear transcription by eukaryotic DNA-dependent RNA polymerases

Contact Info

Product

Resources

About