Abstract:Complex metabolic components in mango leaves lead to high difficulty in RNA extraction. In order to improve the quality of total RNA extraction, on the basis of the existing methods reported in the literature, an RNA extraction method combining acetone washing liquid nitrogen abrasive material with 0.3 mol/L lithium chloride (LiCl) solution and 2 times the volume of anhydrous ethanol as the second precipitator was developed. The experimental results showed that the improved method significantly reduced the amo… Show more
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