A Modified Method for the Rapid Analysis of Long-Chain Alkanes and Neophytadiene from Tobacco

Abstract

A rapid method for the analysis of aliphatic hydrocarbons and neophytadiene in cured tobacco was developed. Briefly, ground tobacco was extracted for 15 min with methylene chloride in a flask placed in an ultrasonic vibration bath, 3-Methyltricosane was added as the internal standard. The solution was filtered and the solvent removed. The sample was redissolved in hexane and chromatographed on a small silicic acid column. The hydrocarbonneophytadiene fraction that eluted with hexane was reduced in volume and analysed on a SE-54 wall-coated glass capillary column, which resolved and permitted quantitation of the normal, iso-methyl- branched, and anteiso-methyl-branched hydrocarbons. The analytical data obtained by this method were equal to those obtained by the conventional Soxhlet extraction procedure. Based on experience in our laboratory, analysis time was reduced about fivefold and isomeric hydrocarbons were resolved by capillary gas chromatography.

Isolation and Identification of the Wax Esters from the Cuticular Waxes of Green Tobacco Leaf

“…Note the designations for the iso-chain [i], anteiso-chain [a], and normal-chain [n] wax ester isomers and their elution order from the SE-54 capillary column. The elution order of iso-, anteiso-, and normal-chain esters is consistent with the elution order of aliphatic hydrocarbons with similar chain branching, also found in the cuticular waxes of tobacco leaf (4,13). The data are more clearly depicted in selected single ion profiles of the molecular ions of the wax esters (Fig.…”

The Application of Capillary Gas Chromatography to the Analyses of Acidic Constituents of Tobacco Leaf and Smoke

“…The non-polar stationary phase, SE-54, was found to be the most useful liquid phase for analyses of tobacco and smoke components (1,4,6,7,9,10,12). The trimethylsilyl derivatives (TMS) of the mono-and dihydroxybenzenes from tobacco smoke and the phenolic acids of leaf and smoke can · be readily analyzed on fused silica SE-54 wall-coated open-tubular columns ( 4 ).…”

Προσδιορισμος Ενωσεων Που Εμπεριεχονται Σε Ελληνικα Καπνα Επεξεργασμε- Να Και Μηπριν Και Μετα Την Καυση Αυτων

“…Γεγονός που άλλωστε προκύπτει και από τον έλεγχο των πινάκων (8.4)-(8.9), που παρουσιάζουν τα ταυτοποιημένα συστατικά. [99][100][101][102] Το ποσοστό που εμφανίζονται αυτές οι κατηγορίες ενώσεων, έχει άμεση σχέση με την υπάρχουσα υγρασία στα αρχικά φύλλα. Συγκεκριμένα, η παρουσία σημαντικών ποσοτήτων υγρασίας στ' αρχικά φύλλα καπνού, είναι η αιτία για την παρουσία χαμηλών συγκεντρώσεων καρβονυλικών συστατικών.…”