A modified method based on p-nitrophenol assay to quantify hydrolysis activities of lipases in litters

Farnet, Anne‐Marie; Qasemian, Leila; Goujard, Laurent; Gil, G.; Guiral, Daniel; Ruaudel, Florence; Ferre, E.

doi:10.1016/j.soilbio.2009.11.015

Cited by 18 publications

(13 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, we were able to more accurately determine the interactions between substrates (i.e. both water and p-nitrophenyl laurate), enzymes and the vegetal matrix which drive enzyme activity rates under low water content conditions (Farnet et al, 2010;Goujard et al, 2009). We did not compare lipase activities in the two types of litters quantitatively, since variations in the chemical composition of litter are known to shape microbial community structure and in turn the amount of extracellular enzymes produced (Fioretto et al, 2009;Papa et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

“…Enzyme activities were measured from litters for different a w values as described by Farnet et al (2010). This assay was adapted for litters from the method of Pencreac'h and Baratti (1996) using methyl t-butyl ether as the organic solvent of the reaction mixture.…”

Section: Lipase Hydrolytic Activitiesmentioning

confidence: 99%

“…Our previous study, (Farnet et al, 2013), first described the pronounced effect this parameter has on the balance of certain enzyme reactions (hydrolysis/synthesis), and the consequences for carbon mineralization or storage. Certain enzymes such as lipases are indeed able to catalyze either hydrolysis or synthesis according to water availability, since water is a substrate of the reaction (Goujard et al, 2009;Farnet et al, 2010). Thus, under the particular conditions linked to arid climates, water activity can be considered as a useful parameter to clearly understand enzyme activity dynamics.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Infra-red spectroscopy reveals chemical interactions driving water availability for enzyme activities in litters of typical Mediterranean tree species

Silva

Ferre

Dupuy

et al. 2017

Soil Biology and Biochemistry

View full text Add to dashboard Cite

Rebufa. Infra-red spectroscopy reveals chemical interactions driving water availability for enzyme activities in litters of typical Mediterranean tree species. Soil Biology and Biochemistry, Elsevier, 2017, 114, pp.72-81. 10 In Mediterranean ecosystems, water is one of the main drivers of the microbial activities that support organic matter turnover in soils or litters. In addition to drought stress, coastal areas are subject to osmotic stress linked to sea spray exposure. Here we explored i) how water availability, characterized by water activity a w , is impacted by adding NaCI to litter, ii) the chemical interactions between water, NaCl and the litter matrix and iii) whether microbial activities (using lipase as a model) are affected under these conditions. Litters of two vegetal species typical of the Mediterranean area (Quercus pubescens and Pinus halepensis) were subjected to FTIR-ATR (Fourier-Transformed Infra-Red e Attenuated Total Reflectance) spectroscopy for chemical characterization. Q. pubescens and P. halepensis litters were characterized by cutin and aromatics respectively. Sorption isotherms were identical for both species litters; when NaCl was added, a shift in isotherm shape was observed at a w ranging from 0.75 to 1. FTIR also discriminated samples with and without added NaCl and revealed that cellulose is probably the polymer in interactions with ions. Very interestingly, no differences were found between lipase hydrolytic activities with and without added NaCl: salt addition had no effect on these activities.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Lipase Hydrolytic Activitiesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Infra-red spectroscopy reveals chemical interactions driving water availability for enzyme activities in litters of typical Mediterranean tree species

Silva

Ferre

Dupuy

et al. 2017

Soil Biology and Biochemistry

View full text Add to dashboard Cite

show abstract

“…Lipase activity was measured in accordance with Farnet et al . () with p ‐nitrophenyl ( p NP) laurate as substrate. Protease activity was determined using the method of Ladd & Butler ().…”

Section: Methodsmentioning

confidence: 99%

Effect of agricultural practices and coastal constraints on soil microbial functional properties in Mediterranean olive orchards

Boukhdoud

Gros

Darwish

et al. 2016

European J Soil Science

View full text Add to dashboard Cite

International audienceThis research examines how agricultural practices commonly used in Lebanese olive tree orchards (no-tillage, co-culture with Vicia sativa L. var. sativa and conventional tillage) affect soil microbial and chemical characteristics, and whether this depends on geographical context (coastal or inland areas). Four coastal and four inland sampling sites were selected, and at each site three practices were considered (one practice per plot). For each plot (200m(2)), 20 soil samples were taken and homogenized to obtain a composite sample from which soil was characterized chemically and microbiologically. Differences in chemical properties (smaller N content and alkyl C fraction in the coastal area) depended on geographical location rather than on agricultural practices. For microbial properties, catabolic structure varied with geographical location; the index of catabolic diversity was larger in the coastal area. Importantly, the basal respiration was similar in both areas although the soil contained less nitrogen (N) in the coastal area. The negative effect of conventional tillage on microbial functioning of the soil was observed only in relation to geographical location. We found that this effect was reduced by co-culture, for example with Vicia sativa L. var. sativa. Our study reveals that geographical location affects how agricultural practices affect soil properties (in the coastal area, enhanced mineralization of labile C and change in microbial catabolic profile). This suggests that soil management should take into account the environmental conditions specific to coastal areas, which enhance the adverse effects of conventional tillage. Consequently, this type of management should not be implemented in coastal areas of the Mediterranean

show abstract

“…Lipase, urease and protease activities were measured directly in the soil. The hydrolysis activity of lipases was measured according to Farnet et al (2010b) using p-nitrophenyl laurate as substrate dissolved in an organic solvent. The release of p-nitrophenol was measured at 412 nm after 2 h-incubation at 30 C. The urease activity was measured using the method of Kandeler and Gerber (1988) based on the determination of NH þ 4 after enzyme action on urea at 37 C for 2 h. Protease activity was determined using 1 g of dry soil equivalent in a 2% casein solution in tris(hydroxymethyl) aminomethane buffer (50 mM, pH 8.1) incubated for 3 h at 50 C. After incubation, the reaction was stopped using trichloroacetic acid (TCA) 15% and the mixture was centrifuged for 10 min at 10 000 rpm.…”

Section: Enzymatic Activitiesmentioning

confidence: 99%

Fate of carbamazepine and anthracene in soils watered with UV-LED treated wastewaters

et al. 2013

View full text Add to dashboard Cite

A modified method based on p-nitrophenol assay to quantify hydrolysis activities of lipases in litters

Cited by 18 publications

References 15 publications

Infra-red spectroscopy reveals chemical interactions driving water availability for enzyme activities in litters of typical Mediterranean tree species

Infra-red spectroscopy reveals chemical interactions driving water availability for enzyme activities in litters of typical Mediterranean tree species

Effect of agricultural practices and coastal constraints on soil microbial functional properties in Mediterranean olive orchards

Fate of carbamazepine and anthracene in soils watered with UV-LED treated wastewaters

Contact Info

Product

Resources

About