A modified live PRRSV vaccine and the pathogenic parent strain induce regulatory T cells in pigs naturally infected with Mycoplasma hyopneumoniae

LeRoith, Tanya; Hammond, Sarah; Todd, S. Michelle; Ni, Yanyan; Cecere, Thomas E.; Pelzer, Kevin D.

doi:10.1016/j.vetimm.2010.12.011

Cited by 39 publications

(33 citation statements)

References 26 publications

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“…Our results suggest that mutations in the vaccine strain that result in attenuation of the virus do not alter the virus's ability to stimulate T regs (LeRoith T, 2011). This information can help us design vaccines in which the T reg -stimulating epitopes can be mutated or deleted in order to stimulate a robust virus-specific IFN- response, and provide protection against heterologous strains (LeRoith T, 2011 …”

Section: Immune Modulation Mediated By Interleukin-10mentioning

confidence: 82%

“…Which components of the virus are responsible for T reg proliferation are unknown, but are currently under investigation by our group. Importantly, we were able to demonstrate that T reg induction by PRRSV results in increased susceptibility to natural Mycoplasma hyopneumoniae infection (LeRoith T, 2011). In this study, pigs were inoculated with a virulent strain of PRRSV and a modified live vaccine that was derived from the same strain.…”

Section: Immune Modulation Mediated By Interleukin-10mentioning

confidence: 90%

See 1 more Smart Citation

Regulatory T Cells and Viral Disease

LeRoith¹,

Ahmed²

2012

Inflammation, Chronic Diseases and Cancer - Cell and Molecular Biology, Immunology and Clinical Bases

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Section: Immune Modulation Mediated By Interleukin-10mentioning

confidence: 82%

Section: Immune Modulation Mediated By Interleukin-10mentioning

confidence: 90%

Regulatory T Cells and Viral Disease

LeRoith¹,

Ahmed²

2012

Inflammation, Chronic Diseases and Cancer - Cell and Molecular Biology, Immunology and Clinical Bases

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“…However, MLV-induced immunity is usually delayed and does not provide complete protection against heterologous viruses [8,33]. Similar to the virulent PRRSV strains, MLV also induces Tregs which affect the immunocompetency of the host [17]. It is envisaged that priming with DNA vaccine should reduce MLVinduced immunomodulatory activities (if any) and, subsequently, improve vaccine-induced protective immunity.…”

Section: Discussionmentioning

confidence: 99%

“…Interleukin-10 is essential for induction of PRRSV-specific Tregs [15]. The role of PRRSV-specific Tregs has also been linked to delayed induction of cellular responses and increased susceptibility of bacterial infection in infected hosts [5,16,17].…”

Section: Introductionmentioning

confidence: 99%

A novel DNA vaccine for reduction of PRRSV-induced negative immunomodulatory effects: A proof of concept

Suradhat

Wongyanin

Kesdangsakonwut

et al. 2015

Vaccine

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Please cite this article in press as: Suradhat S, et al. A novel DNA vaccine for reduction of PRRSV-induced negative immunomodulatory effects: A proof of concept. Vaccine (2015), http://dx.Keyword: PRRSV DNA vaccine Nucleocapsid protein Tregs a b s t r a c tBackground: Viral-induced interleukin (IL)-10 and regulatory T lymphocytes (Tregs) are believed to play a major role in shaping the immunological and clinical outcomes following Porcine Reproductive and Respiratory Syndrome virus (PRRSV) infection. Recently, it has been shown that PRRSV nucleocapsid (N) protein can induce IL-10 production which is essential for induction of PRRSV-specific Tregs. We hypothesized that immunity to N protein should reduce PRRSV-induced negative immunomodulatory effects which will be essential for establishing proper anti-PRRSV immunity in infected pigs. Objectives: To investigate the immunomodulatory effects of DNA vaccine encoding a linearized, truncated form of PRRSV-N protein (pORF7t) which was designed to preferentially induce cell-mediated immunity against PRRSV N protein.Method: Immunomodulatory effects of the novel DNA vaccine were investigated in an experimental vaccinated-challenged model. In addition, long-term immunomodulatory effects of the DNA vaccine were investigated in vaccinated pigs kept at the PRRSV-positive environment until the end of the fattening period. Pigs were vaccinated either prior to or following natural PRRSV infection. Result: The results indicated that pORF7t could modulate the anti-PRRSV immune responses and promote the control of viral replication in the vaccinated-challenged pigs. Immunized pigs exhibited increased numbers of PRRSV-specific activated CD4 + CD25 + lymphocytes, reduced numbers of PRRSV-specific Tregs, and rapid viral clearance following infection. In a long-term study, regardless of the time of vaccination, DNA vaccine could modulate the host immune responses, resulted in enhanced PRRSV-specific IFN-␥ producing cells, and reduced numbers of PRRSV-specific Tregs, without evidence of enhanced antibody responses. No vaccine adverse reaction was observed throughout the study. Conclusion: This study revealed the novel concept that PRRSV-specific immunity can be modulated by induction of cell-mediated immunity against the nucleocapsid protein. This concept could potentially benefit the development of PRRSV management and control strategies.

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“…MLV vaccinations in sows reported to cause acute PRRS-like clinical signs, characterized by increased late term abortions, increased numbers of stillborns and mummified piglets, as well as reduced numbers of live born and weaned piglets (Bøtner et al, 1997;Dewey et al, 1999). An additional problem is that the MLV vaccination has been shown to decrease the efficacy of Mycoplasma hyopneumoniae vaccines (Thacker et al, 2000;Le Roitha et al, 2011).…”

Section: Vaccination Programmesmentioning

confidence: 99%

Porcine Herd Health Management Practices for the Control of PRRSV Infection

Papatsiros¹

2012

A Bird's-Eye View of Veterinary Medicine

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A modified live PRRSV vaccine and the pathogenic parent strain induce regulatory T cells in pigs naturally infected with Mycoplasma hyopneumoniae

Cited by 39 publications

References 26 publications

Regulatory T Cells and Viral Disease

Regulatory T Cells and Viral Disease

A novel DNA vaccine for reduction of PRRSV-induced negative immunomodulatory effects: A proof of concept

Porcine Herd Health Management Practices for the Control of PRRSV Infection

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