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The protein fractions of 7-18 day chick serum, allantoic and amniotic fluid and egg white were demonstrated by acrylamide gel electrophoresis.The similarity in protein fractions suggests that most of the amniotic fluid proteins are derived from egg white while serum contributes most of the allantoic fluid proteins.Several protein fractions which are unique to the respective fluids may be produced by components of the extraembryonic membranes.The nature of the proteins found in both serum and allantoic fluid suggests a selective transfer of protein between the two fluids.An interest in the function of the chorioallantoic membrane of the chick embryo has prompted investigations (Conklin, '66) of the membrane and the allantoic Jluid. One of the unanswered questions about the allantoic fluid is the initial source of its primary componcnts. It has been suggested (Romanoff, '60) that avian allantoic fluid is produced either by the allantoic membrane or excreted into the cloaca and thence into the allantoic lumen ( o p . c i t . ) , while the amniotic fluid may originate as a transudate of serum or as a secretory product of the amnion.An obvious way to determine whether serum might contribute to the formation of either fluid would be by comparison of the protein content of the serum and the amniotic and allantoic fluids. Strangely, however, no successful comparison of the protein components of these fluids and of embryonic serum has been made. Indeed, earlier attempts to detect similarities between the fluids and embryonic and adult serum, using paper electrophoresis, failed to demonstrate any proteins in amniotic fluid until after the thirteenth day when, following the rupture of the seroamniotic plate, the proteins of amniotic fluid were identical to ovalbumin (Marshall and Deutsch, '5.0). These authors were able to demonstrate nine protein fractions in 13 day chick serum.Shepard and Hottle ('49) also employed paper electrophoresis and demonstrated three protein fractions in 13 day allantoic fluid. These fractions reportedly were unlike any found in embryonic plasma, J. EXP. ZOOL., 162: 257-262.
The protein fractions of 7-18 day chick serum, allantoic and amniotic fluid and egg white were demonstrated by acrylamide gel electrophoresis.The similarity in protein fractions suggests that most of the amniotic fluid proteins are derived from egg white while serum contributes most of the allantoic fluid proteins.Several protein fractions which are unique to the respective fluids may be produced by components of the extraembryonic membranes.The nature of the proteins found in both serum and allantoic fluid suggests a selective transfer of protein between the two fluids.An interest in the function of the chorioallantoic membrane of the chick embryo has prompted investigations (Conklin, '66) of the membrane and the allantoic Jluid. One of the unanswered questions about the allantoic fluid is the initial source of its primary componcnts. It has been suggested (Romanoff, '60) that avian allantoic fluid is produced either by the allantoic membrane or excreted into the cloaca and thence into the allantoic lumen ( o p . c i t . ) , while the amniotic fluid may originate as a transudate of serum or as a secretory product of the amnion.An obvious way to determine whether serum might contribute to the formation of either fluid would be by comparison of the protein content of the serum and the amniotic and allantoic fluids. Strangely, however, no successful comparison of the protein components of these fluids and of embryonic serum has been made. Indeed, earlier attempts to detect similarities between the fluids and embryonic and adult serum, using paper electrophoresis, failed to demonstrate any proteins in amniotic fluid until after the thirteenth day when, following the rupture of the seroamniotic plate, the proteins of amniotic fluid were identical to ovalbumin (Marshall and Deutsch, '5.0). These authors were able to demonstrate nine protein fractions in 13 day chick serum.Shepard and Hottle ('49) also employed paper electrophoresis and demonstrated three protein fractions in 13 day allantoic fluid. These fractions reportedly were unlike any found in embryonic plasma, J. EXP. ZOOL., 162: 257-262.
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