A modified coomassie brilliant blue G 250 staining method for the detection of chitinase activity and molecular weight after polyacrylamide gel electrophoresis

Liau, Chun Yi; Lin, Chung‐Sheng

doi:10.1263/jbb.106.111

Cited by 26 publications

(13 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The anthrone colorimetry method was adopted to determine the content of soluble sugars (Shi et al 2011). Coomassie brilliant blue G 250 staining was used for the detection of proteins (Liu and Lin 2008). Organic acid content was determined by acid-base titration, and the acid washing method was used for crude fiber content analysis (Xie and Qu 2006).…”

Section: Methodsmentioning

confidence: 99%

Directional transfer of a multiple-allele male sterile line in Brassica campestris L. ssp. chinensis (L.) Makino var. rosularis Tsen et Lee

Wang¹,

Zhang²,

Li³

et al. 2014

Breed. Sci.

View full text Add to dashboard Cite

To produce hybrid seeds of Wutacai (Brassica campestris L. ssp. chinensis (L.) Makino var. rosularis Tsen et Lee), a “directional transfer program” was designed to breed the multiple-allele male sterile line of Wutacai. A multiple-allele male sterile line of Naibaicai (Brassica campestris L. ssp. chinensis L., S01) was used as the male sterile resource, and an inbred line of Wutacai (WT01) was used as the target line. Recurrent backcrossing was employed to transfer the male sterility and other botanical traits simultaneously, while the genotype was identified through test crossing. The male sterility was successfully transferred from S01 to WT01. A new male sterile line, GMS-3, with similar botanical traits to WT01, was bred. Four hybrid combinations were generated with GMS-3 as the female parent. One hybrid (C1) that contained the most desirable traits was developed from the new male sterile line.

show abstract

Section: Methodsmentioning

confidence: 99%

Directional transfer of a multiple-allele male sterile line in Brassica campestris L. ssp. chinensis (L.) Makino var. rosularis Tsen et Lee

Wang¹,

Zhang²,

Li³

et al. 2014

Breed. Sci.

View full text Add to dashboard Cite

show abstract

“…The soy proteins concentration in the wastewater was determined by Coomassie Brilliant Blue protein assay. 19 The standard curve was worked out using BSA as reference protein at the maximum absorption wavelength 595 nm. The linearfitting equation was A = 6.475C + 0.0562, r 2 = 0.9994, and the range of C was from 0.01 g/L to 0.1 g/L, where A and C were the absorption value at λ = 595 nm and the soy proteins concentration, respectively.…”

Section: Methodsmentioning

confidence: 99%

Recovery of Isoflavones from the Soy Whey Wastewater Using Two-Stage Batch Foam Fractionation

Liu

Wang

et al. 2013

Ind. Eng. Chem. Res.

View full text Add to dashboard Cite

The feasibility of recovering bioactive substances without surface activity from their aqueous solutions was studied using biosurfactants as collectors. For recovering the isoflavones from the soy whey wastewater, a two-stage batch foam fractionation was developed using the soy proteins as collectors. The results showed that when the conditions of the first stage were temperature of 60°C, pH of 5.0, volumetric air flow rate 80 of mL/min, and loading liquid volume of 400 mL, a high isoflavones enrichment ratio of 4.05 was obtained and the residual solution could be used as the feeding solution of the second stage. When the conditions of the second stage were temperature of 25°C, pH of 5.0, and volumetric air flow rate of 150 mL/ min, the soy proteins concentration in the residual solution could decrease to 622 mg/L. By reusing the foamate of the second stage as the feeding solution of the first stage, the total isoflavones recovery percentage reached 87.72%.

show abstract

“…The concentration of WSP was measured by Coomassie Brilliant Blue Protein assay [17]. 1 mL of the test protein solution was mixed into 5 mL of the prepared Coomassie Brillant Blue solution (200 mg of Coomassie Brilliant Blue G-250 were dissolved in 100 mL of 95% ethanol and then 200 mL of 85% phosphoric acid were added.…”

Section: Determination Of the Wsp Concentrationmentioning

confidence: 99%

Isolation of soy whey proteins from isoflavones in the concentrated solution using foam fractionation

Liu

Wang

et al. 2015

Separation and Purification Technology

View full text Add to dashboard Cite

A modified coomassie brilliant blue G 250 staining method for the detection of chitinase activity and molecular weight after polyacrylamide gel electrophoresis

Cited by 26 publications

References 31 publications

Directional transfer of a multiple-allele male sterile line in <i>Brassica campestris</i> L. ssp. <i>chinensis</i> (L.) Makino var. <i>rosularis</i> Tsen et Lee

Directional transfer of a multiple-allele male sterile line in <i>Brassica campestris</i> L. ssp. <i>chinensis</i> (L.) Makino var. <i>rosularis</i> Tsen et Lee

Recovery of Isoflavones from the Soy Whey Wastewater Using Two-Stage Batch Foam Fractionation

Isolation of soy whey proteins from isoflavones in the concentrated solution using foam fractionation

Contact Info

Product

Resources

About