A modified assay for the detection of human adult active rosette forming lymphocytes

Smith, Randall A.; Kerman, Ronald H.; Ezdinli, Ediz Z.; Stefani, Stefano S.

doi:10.1016/0022-1759(75)90094-0

Cited by 49 publications

(16 citation statements)

References 16 publications

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“…The differences found in the percentage of ERFCs were given only by'different lymphocytes /SRBCs ratios as described also by Smith et al (1975) and Woody and Sell (1975). .…”

Section: Discussionmentioning

confidence: 80%

The Percentage of Active E rosette Forming Cells After Incubation of Pig Lymphocytes in Xenogeneic Sera

Knotek¹,

Vojtíšek²,

Hořín³

et al. 1993

Acta Vet. Brno

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“…The differences found in the percentage of ERFCs were given only by'different lymphocytes /SRBCs ratios as described also by Smith et al (1975) and Woody and Sell (1975). .…”

Section: Discussionmentioning

confidence: 80%

The Percentage of Active E rosette Forming Cells After Incubation of Pig Lymphocytes in Xenogeneic Sera

Knotek¹,

Vojtíšek²,

Hořín³

et al. 1993

Acta Vet. Brno

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“…For the demonstration of 'active' E-rosette forming cells (active E-RFC) 0.1 ml of 1% E was added, and the tubes were centrifuged at 200 g for 5 min [12]. For the demonstra tion o f 'total' E-RFC 0.1 ml of 0.5% E was added, and the tube in cubated at 37°C for 5 min before centrifugation at 200 g for 5 min and incubation in ice water overnight [7], In both procedures, most of the supernatant was carefully sucked off and the cell pellet gently suspended in a Pasteur pipette.…”

Section: Methodsmentioning

confidence: 99%

Enhancement of E Rosette Formation by Incubation of Lymphocytes in Cerebrospinal Fluid Dilutions

Naess¹

1982

Int Arch Allergy Immunol

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Lymphocytes from normal donors were incubated for 4 h at 37 °C in dilutions of normal cerebrospinal fluid (CSF) in Hanks’ balanced salt solution (HBSS), before being tested for ‘active’ and ‘total’ sheep erythrocyte rosette-forming cells (E-RFC). Incubation in CSF dilutions caused an increase in the percentage of both ‘active’ and ‘total’ E-RFC compared to incubation in HBSS. Incubation in HBSS caused a decrease in ‘total’ but not in ‘active’ E-RFC percentages. CSF thus effects an increase in the ability to form E-RFC under sub-optimal conditions. No mitogenic effect of CSF was observed in lymphocyte culture experiments.

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“…( [14] modified technique, the isolated lymphocytes were tested with sheep red blood cells previous activation. Data were assumed as Ea-RFC percent.…”

Section: Methodsmentioning

confidence: 99%

Immunobiology of Primary CNS Tumors in Infancy and Childhood

Gerosa¹,

Amadori²,

Semenzato³

et al. 1980

Pediatr Neurosurg

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The authors report their experience on the immunological monitoring of children with posterior fossa medulloblastomas. The most important findings concerning humoral and cell-mediated immunity in this kind of patient are discussed. Among the main immunobiological pictures, the authors stress the remarkable failure of the T-cell dependent immune response, generally correlated to the degree of malignancy of the tumor, and the characteristic appearance of cells with natural cytotoxic activity, whose precise outlining is, at present, in progress. The B-cell dependent pool, mostly investigated through immunofluorescence studies, turned out to be very close to normal.

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A modified assay for the detection of human adult active rosette forming lymphocytes

Cited by 49 publications

References 16 publications

The Percentage of Active E rosette Forming Cells After Incubation of Pig Lymphocytes in Xenogeneic Sera

The Percentage of Active E rosette Forming Cells After Incubation of Pig Lymphocytes in Xenogeneic Sera

Enhancement of E Rosette Formation by Incubation of Lymphocytes in Cerebrospinal Fluid Dilutions

Immunobiology of Primary CNS Tumors in Infancy and Childhood

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