A Modified Approach to Evaluation of DON Content in Scab-Damaged Ground Wheat by Use of Diffuse Reflectance Spectroscopy

Fusarium damage assessment in wheat kernels by Vis/NIR hyperspectral imaging

“…Conventional methodologies for measurement of DON concentration (typically in the sub-ppm to 10 ppm range), such as immunoassay or mass spectrometry, are effective but slow and therefore not well suited to grain inspection operations. Near-infrared (NIR) spectroscopy has been examined as an alternative procedure [7][8][9][10][11]; however, the relatively low concentration range for DON makes it very challenging for NIR quantitative analysis (typically developed on analytes one hundred times or greater in concentration). As an alternative to measurement of DON, some NIR studies have relied on the positive, though imperfect correlation between the visual appearance of Fusarium damage and DON [12].…”

REVIEW: An Overview on Fusarium Mycotoxins in the Durum Wheat Pasta Production Chain

“…A semi-quantitative model was developed to discriminate between blank and naturally contaminated wheat samples by setting a cutoff at 300 μg/kg (De Girolamo et al 2009). A modified approach to evaluate DON content in scab-damaged wheat by using diffuse reflectance UV-vis spectroscopy was reported recently (Siuda et al 2008).…”

Estimating Deoxynivalenol Content of Ground Oats Using VIS‐NIR Spectroscopy

“…Previous studies have shown that Fusarium-damaged and sound wheat kernels differ in their major food reserves and water contents (Boyacioglu and Hettiarachchy 1995;Delwiche 2003;Jackowiak et al 2005;Wang et al 2005). Therefore, spectral differences between sound and scabby kernels were attributed to differences in major seed constituents rather than DON content per se, due to the extremely low concentration of the toxin and because of overlap of bands in the NIR region from major seed constituents (Siuda et al 2008;Girolamo et al 2009;Liu et al 2009). Even though it was difficult to attribute specific spectral features to DON, the overall effects of Fusarium infection on the physical, chemical, and structural attributes of kernels were correlated to DON contamination and were used to estimate DON level of samples (Siuda et al 2008;Girolamo et al 2009;Liu et al 2009).…”

“…The accuracy of spectroscopic methods in estimating DON contamination is highly dependent on the relationship between degree of scab damage and the actual DON level of the investigated samples (Siuda et al 2008). Apparently healthy-looking kernels can be contaminated with considerable levels of DON, and kernels with visible scab damage are not necessarily contaminated with corresponding high levels of DON (Dexter and Nowicki 2003).…”