A modified antigen-antibody crossed electrophoresis characterizing the specificity and titre of human precipitins against Candida albicancs

Svendsen, P. Just; Axelsen, N. H.

doi:10.1016/0022-1759(72)90044-0

Cited by 73 publications

(19 citation statements)

References 3 publications

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“…Much information exists regarding the environmental factors that control the yeast-tomycelium transition (Lee et al, 1975;Mitchell & Soll, 1979;Manning & Mitchell, 1980a;Mattia et al, 1982), and several studies have also been made concerning the biochemical (Chiew et al, 1980;Sullivan et al, 1983) and antigenic differences between the two types of cells (Chattaway et al, 1968(Chattaway et al, , 1976Svedsen & Axelsen, 1972;Manning & Mitchell, 1980b, c) as well as the cytology of the process (Soll & Mitchell, 1983;Gow & Gooday, 1984). However, a deeper understanding of the biology and pathogenicity of C. albicans has been hampered by the difficulties in carrying out genetic analysis in this species.…”

Section: Introductionmentioning

confidence: 99%

Genetic Analysis of Candida albicans Morphological Mutants

Pomés¹,

Gil²,

Nombela³

1985

Microbiology

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Section: Introductionmentioning

confidence: 99%

Genetic Analysis of Candida albicans Morphological Mutants

Pomés¹,

Gil²,

Nombela³

1985

Microbiology

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“…The intermediate gel in crossed immunoelectrophoretic analysis was introduced by Svendsen and Axelsen [1972] as a tool for measurements of human precipitins against Candida albicans antigens. The ma jor advantage over other methods of precip itin analysis is the high resolution and quan titative information gained.…”

Section: Discussionmentioning

confidence: 99%

Occurrence of Specific Precipitins against Bovine Whey Proteins in Serum from Children with Gastrointestinal Disorders

Løwenstein¹,

Krasilnikoff²,

Bjerrum³

et al. 1977

Int Arch Allergy Immunol

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Children with cow’s milk intolerance, clinically non-confirmed cow’s milk intolerance, coeliac disease, non-confirmed malabsorption, other gastrointestinal disorders, and normal children were investigated for the presence of precipitins against 40 individual bovine whey proteins by means of the crossed immunoelectrophoresis with intermediate gel technique. In the various groups of children the amount and specificity of bovine whey precipitins were neither connected with the various gastrointestinal disorders nor with alternating diet and challenge with milk. Bovine whey-specific IgE could not be detected in any of the patients clinically suspected for cow’s milk intolerance by cutaneous test, RAST or CRIE. The immunoglobulin level of the patients investigated did not differ significantly from the normal ranges. It is concluded that investigations of serum precipitins against bovine whey proteins do not give any significant information concerning cow’s milk intolerance.

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“…Rocket Immunoelectrophoresis C4d was quanti fied by rocket immunoelectrophoresis using interme diate gel technique [Svendsen and Axelsen, 1972;Brandslund et ah, 1981 andFolkersen et al, 1985], The design of the plates and description of the method are given in figure 1.…”

Section: Methodsmentioning

confidence: 99%

Immunoelectrophoretic Analysis of C4 Split Products Expressing D but Not C Epitopes: Influence of Storage, Ca2+ and Ca2+-Chelating Agents

Ne¹,

Folkersen²,

Teisner³

et al. 1985

Complement

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Based on immunoelectrophoretic methods a heterogeneity in the electrophoretic mobility of C4d was observed. C4d was defined immunochemically as C4 molecules expressing D but lacking C epitopes. A beta-mobile form was observed when EDTA or heparin was not added to the sample prior to electrophoretic analysis. This component was generated during electrophoresis. Another C4d component migrating to the post-albumin region probably represented an in vivo generated split product. However, this C4d form was also produced during storage of serum or plasma at room temperature and its formation was enhanced in the presence of EDTA. Based on these findings standard conditions for collection and storage of clinical samples for quantification of C4d by electroimmunoassay are suggested.

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A modified antigen-antibody crossed electrophoresis characterizing the specificity and titre of human precipitins against Candida albicancs

Cited by 73 publications

References 3 publications

Genetic Analysis of Candida albicans Morphological Mutants

Genetic Analysis of Candida albicans Morphological Mutants

Occurrence of Specific Precipitins against Bovine Whey Proteins in Serum from Children with Gastrointestinal Disorders

Immunoelectrophoretic Analysis of C4 Split Products Expressing D but Not C Epitopes: Influence of Storage, Ca2+ and Ca2+-Chelating Agents

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