A modified Amaranthus betacyanin bioassay for the rapid determination of cytokinins in plant extracts

Biddington, N. L.; Thomas, T. H.

doi:10.1007/bf00386279

Cited by 214 publications

(74 citation statements)

References 3 publications

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“…3 The effects of auxin on gene activity, especially in the epidermis, explains the quantitative variation promoted by addition of auxin. 12 Auxins may affect the kind of proteins formed in a plant cell before or as soon as growth promotion starts, which could explain changes in the level of some substances through the modification of the cell enzymatic pattern. 13 The effect of cytokinins on the levels of secondary metabolites was already observed.…”

Section: Resultsmentioning

confidence: 99%

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Essential oil composition of Melissa officinalis L. in vitro produced under the influence of growth regulators

Silva¹,

Sato²,

Lage³

et al. 2005

J. Braz. Chem. Soc.

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Foram investigados os efeitos de ácido indol acético (11,42 μmol L -1 ) e benzilaminopurina (8,87 μmol L -1 ) sobre o crescimento e composição do óleo essencial de plantas in vitro de Melissa officinalis. Plantas desenvolvidas em meio de Murashige e Skoog (MS) apresentaram o incremento de 1,4 vezes na proporção de nerol e de 4,1vezes de geraniol, quando comparadas às plantas ex vitro. Tratamentos com 11,42 μmol L -1 de ácido indol acético mais 8,87 μmol L -1 de benzilaminopurina resultaram em aumentos de 1,7 e 2,2 vezes na proporção de nerol e geraniol, respectivamente, em plantas de 60 dias. Estes aumentos podem estar associados à ação dos reguladores de crescimento, por estimularem o desenvolvimento vegetal (na organogênese e alongamento de brotos) e retardarem a oxidação de álcoois em aldeídos.It was investigated the effects of indole-3-acetic acid (11.42 μmol L -1 ), benzylaminopurine (8.87 μmol L -1 ) on essential oil composition and on the growth of Melissa officinalis in vitro plants. In vitro plantlets developed on MS media, showed 1.4 times in the proportion of nerol and 4.1 of geraniol, when compared with ex vitro plants. Treatments with 11.42 μmol L -1 indole-3-acetic acid plus 8.87 μmol L -1 benzylaminopurine led to 1.7 and 2.2 fold in proportion of nerol and geraniol, respectively in 60-day-old whole plants. These increases might be associated with the action of growth regulators wich stimulate plant growth (shoot organogenesis and elongation) and delaying the alcohol oxidation to aldehydes. Keywords: Melissa officinalis, essential oil composition, VOCs, GC/MS, growth regulators, tissue culture IntroductionMelissa officinalis Lam. (Lamiaceae) also known as lemon balm, is a perennial herb that presents a lemon flavor. The infusion of its leaves is used in folk medicine due to its sedative and antispasmodic properties. The chemical composition of the essential oil of the M. officinalis leaf (0.02-0.3% dry weight) has been previously studied, being the major compounds citronellal (2-40%) and citral (mixture of neral and geranial: 10-30%), followed by β-caryophyllene, germacrene D, ocimene and citronellol. 1 The growth of sprouts and roots and the composition of the essential leaf oil can be altered by addition of selected phytohormones into the nutrition medium. 2 Culture media supplemented with high concentrations of BA induced Melissa officinalis plants to accumulate more than 10% alloaromadendrene. 3 In order to identify possible factors affecting the oil production and its quality, M. officinalis was cultured in vitro and the effect of growth regulators on the essential oil yield and composition were evaluated by gas chromatography and gas chromatography/mass spectrometry. Experimental Plant materialSeeds acquired from ISLAR, batch Nº 8250 were used to obtain the plant material. Erika Von Sohsten Medeiros Treatment with growth regulatorsNodal segments of in vitro plantlets were cultured in four different media composition: MS0; MS plus 11.42 μmol L -1 Indole-3-acetic acid (IAA); MS plus 8.87 μmol ...

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Section: Resultsmentioning

confidence: 99%

“…13 The effect of cytokinins on the levels of secondary metabolites was already observed. Cytokinins are able to stimulate the synthesis of betacyanins 12 and indole alkaloids. 13 Field plants are exposed to considerable more stressful conditions, such as lower relative humidity, higher light levels, and herbivory those are the more stressful.…”

Section: Resultsmentioning

confidence: 99%

Essential oil composition of Melissa officinalis L. in vitro produced under the influence of growth regulators

Silva¹,

Sato²,

Lage³

et al. 2005

J. Braz. Chem. Soc.

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“…In other words, at the panicle~formation stage, the absorption of nitrogen accounted for more than 60% of the total accumulation in above~ground parts in both Xylem exudates were collected at the base of stems for 12 hours during night. Cytokinin activity was determined by the Amaranthus betacyanin bioassay (Biddington and Thomas, 1973) using Amaranthus tricolor.…”

Section: Discussionmentioning

confidence: 99%

A Comparison of the Accumulation and Partitioning of Nitrogen in Plants between Two Rice Cultivars, Akenohoshi and Nipponbare, at the Ripening Stage

Ookawa

Naruoka

Yamazaki

et al. 2003

Plant Production Science

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“…Xylem sap was weighed immediately after collection, supplemented with 99% ethanol, as a preservative, to a concentration of 80% ethanol, and stored at -20°C until analysis. Cytokinin activity was determined by the Amaranthus betacyanin bioassay (Biddington and Thomas, 1973) using Amaran thus tricolor. The xylem sap in 80% ethanol was evaporated on a rotary evaporator at 40°C to an aqueous residue.…”

Section: Resistance To Water Transportmentioning

confidence: 99%

Effects of Soil Moisture Depletion for One Month before Flowering on Dry Matter Production and Ecophysiological Characteristics of Wheat Plants in Wet Soil during Grain Filling

Emiko

Ookawa

Ishihara

et al. 2003

Plant Production Science

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A modified Amaranthus betacyanin bioassay for the rapid determination of cytokinins in plant extracts

Abstract: Pure cytokinin standards and celery seed extracts containing cytokinin activity were bioassayed using a modified Amaranthus betacyanin bioassay. The assay is very rapid and requires no special sterile precautions.

Cited by 214 publications

References 3 publications

Essential oil composition of Melissa officinalis L. in vitro produced under the influence of growth regulators

Essential oil composition of Melissa officinalis L. in vitro produced under the influence of growth regulators

A Comparison of the Accumulation and Partitioning of Nitrogen in Plants between Two Rice Cultivars, Akenohoshi and Nipponbare, at the Ripening Stage

Effects of Soil Moisture Depletion for One Month before Flowering on Dry Matter Production and Ecophysiological Characteristics of Wheat Plants in Wet Soil during Grain Filling

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