A MODIFIED ALUMINON REAGENT FOR THE DETERMINATION OF ALUMINUM AFTER HNO3–H2SO4 DIGESTION

Wang, Chang; Wood, Frances A.

doi:10.4141/cjss73-035

Cited by 13 publications

(9 citation statements)

References 1 publication

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“…The dry, powdered plant material was mineralized in a nitric-perchloric mixture (3:1, v/v). The Al content was determined using a modified aluminon method (Wang and Wood, 1973).…”

Section: Methodsmentioning

confidence: 99%

The Effects of Aluminium on the Photosynthetic Apparatus of Two Rice Cultivars

et al. 2013

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SUMMARYWe aimed to evaluate aluminium (Al) effects on the photosynthetic apparatus of two rice cultivars with contrasting tolerances to Al. Nine-days-old seedlings were exposed to 0 or 1 mM Al for 10 days, and then dry mass, Al and chloroplastidic pigment contents and photosynthetic parameters were determined. Al accumulated mainly in the roots of the Al-treated plants. In the leaves, Al increased only in the sensitive cultivar, but there was no difference between the cultivars in Al-treated plants. The root and leaf dry mass, the net carbon assimilation rate, stomatal conductance and internal CO2 concentration were all reduced in response to Al application, but only in the sensitive cultivar. Both the initial fluorescence and potential photochemical efficiency of photosystem II were unresponsive to the Al treatments, regardless of the cultivar. In the Al-sensitive cultivar, Al provoked significant decreases in the photochemical quenching coefficient, quantum yield of photosystem II electron transport and apparent electron transport rate, in parallel to an unaltered non-photochemical quenching coefficient. All of these parameters remained at the control levels in the tolerant cultivar. The chloroplastidic pigment content increased only in the Al-tolerant cultivar, whereas it remained unaltered after Al treatment in the sensitive cultivar. In conclusion, Al induced stomatal and (most likely) photochemical constraints on photosynthesis but with no apparent signs of photoinhibition in the Al-sensitive cultivar. Despite the similar Al levels of the cultivars, unchanging biomass accumulation or photosynthetic performance in the tolerant cultivar challenged with Al highlights its higher intrinsic ability to cope with Al stress.

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“…The dry, powdered plant material was mineralized in a nitric-perchloric mixture (3:1, v/v). The Al content was determined using a modified aluminon method (Wang and Wood, 1973).…”

Section: Methodsmentioning

confidence: 99%

The Effects of Aluminium on the Photosynthetic Apparatus of Two Rice Cultivars

et al. 2013

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“…Samples of oven-dried plant materials, finely ground in a stainless steel electric grinder, were digested in a mixture of HNO 3 :HClO 4 (2:1, v/v), and the Al content was determined using the aluminon spectrophotometric method (Wang and Wood, 1973).…”

Section: Determination Of the Aluminum Contentmentioning

confidence: 99%

Antioxidant system response induced by aluminum in two rice cultivars

Ribeiro

Cambraia

Peixoto

et al. 2012

Braz. J. Plant Physiol.

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The antioxidant defense system response was evaluated in two rice cultivars (Oryza sativa L.), Fernandes (CNA-1158) and Maravilha (CNA-6843-1), treated with toxic levels of aluminum. After exposure to aluminum we determined plant growth, H 2 O 2 and O 2•-contents, lipid peroxidation, antioxidant enzymes activities and ascorbate and dehydroascorbate contents. Al predominantly accumulated in roots of both cultivars but it reduced root and shoot growth only in the Maravilha cultivar. Treatment with aluminum resulted in a reduction of 84 and 60% in the levels of H 2 O 2 in the roots of the cultivars Maravilha and Fernandes, respectively, and of 26% in the levels of O 2•-only in the roots of Fernandes cultivar. Increased lipid peroxidation was observed only in the roots of the Maravilha cultivar. In general, the antioxidant enzyme activities were higher in roots and increased in the presence of aluminum, especially in the Fernandes cultivar. The levels of ascorbate were higher in leaves and increased with aluminum treatment, while dehydroascorbate decreased in roots of both cultivars after aluminum treatment. However, the ascorbate/dehydroascorbate ratio increased in the roots of both cultivars after treatment with aluminum. Ascorbate, dehydroascorbate and ascorbate/dehydroascorbate levels found here point to an efficient regeneration of ascorbate, essential for the homeostasis of cellular metabolites involved in reactive oxygen species removal by rice plants treated with aluminum. Therefore, the higher tolerance of Fernandes to aluminum relative to Maravilha cultivar may be the result of better growth of the root system and shoots, higher antioxidant enzyme activities and a best use/ regeneration of ascorbate.

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“…Several samples of clay and silt fractions were treated with 1 M HCl at 80"C, K-saturated and heated at 550°C or Mg-saturated and submitted to EG vapour to complete the determination of chloritic and vermiculitic minerals (Ross, 1969;Brindley & Brown, 1980). Fe, Mg and Mn present in the HC1 extracts were determined by AAS and Al colorimetrically with aluminon (Wang & Wood, 1973). The selective dissolution procedure of Kiely & Jackson (1965) (fusion with Na-pyrosulphate) was used to determine amounts of quartz, orthoclase, albite and anorthite in the silt (2-50 pm) and sand 5Ck2000 pm) fractions.…”

Section: Introductionmentioning

confidence: 99%

Changes caused by Norway spruce in an ochreous brown earth, assessed by the isoquartz method

Sohet

Herbauts

Gruber

1988

Journal of Soil Science

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Previous investigations had shown that about 80 years of Norway spruce monoculture on an ochreous brown earth of the Belgian Ardennes were sufficient to accentuate the weak podzolization process already present in the climax beechwood soil and to increase the weathering of several minerals, e.g. 2:2 clay minerals. In order to evaluate more accurately mineral weathering and spruce-induced losses of nutrient elements, an isoquartz assessment method has been applied to a pair of soil profiles, one developed under beech, the other under spruce.Results show a clear decrease of several weatherable minerals in the soil of the spruce stand which was more marked than in the beechwood, especially for chloritic minerals: losses reach about 30% of the initial chlorite content in the cambic horizon and regularly increase up to 70% in the humic layer. There were severe spruce-induced losses of Mg and Na: 27% of the initial Mg reserve was removed from the whole soil and the losses reached 60% in the upper 20 cm.It is concluded that 86 years of Norway spruce monoculture have appreciably increased weathering processes and removal of elements. 550

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A MODIFIED ALUMINON REAGENT FOR THE DETERMINATION OF ALUMINUM AFTER HNO₃–H₂SO₄ DIGESTION

Abstract: not available

Cited by 13 publications

References 1 publication

The Effects of Aluminium on the Photosynthetic Apparatus of Two Rice Cultivars

The Effects of Aluminium on the Photosynthetic Apparatus of Two Rice Cultivars

Antioxidant system response induced by aluminum in two rice cultivars

Changes caused by Norway spruce in an ochreous brown earth, assessed by the isoquartz method

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