A Modified Alcian Yellow and Toluidine Blue Staining Method to Visualize Intracellular Okadaella Gastrococcus

Okada, Takayuki; Adkins, Graham; Hori, Keiichi; Miwa, Hiroto

doi:10.1016/s0016-5085(11)61285-7

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“…These microorganisms, on the other hand, very closely resemble a newly identified Gram-negative Streptococcaceae : Okadaella gastrococcus (ATCC BAA-2258, NBRC 107862, V10/022878, GenBank HQ699465). 7 It is very interesting to note that intralesional and intracellular O gastrococcus has been found not only in erosive lesions of the gastroduodenal mucosa among H pylori -negative23 and H pylori -positive24 young generations, but also in the classic gastric carcinogenic cascade 25 26. The pathogenesis of the haemorrhagic diffuse erosive gastroduodenitis and multiple superficial ulcerations associated with unidentified coccoid microorganisms may be explained by that of intralesional and intracellular O gastrococcus -associated gastric mucosal lesions 27 28…”

Section: Discussionmentioning

confidence: 99%

Unidentified intralesional and intracellular coccoid microorganism discovered in the young man with a diffuse erosive gastroduodenitis and multiple superficial ulcerations

Okada¹,

Adkins

2014

BMJ Case Reports

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SUMMARYA Helicobacter pylori-negative young Japanese man with dyspeptic symptoms suffered from a diffuse erosive gastroduodenitis and multiple superficial ulcerations. Histology and electron microscopic examinations on the biopsy specimens revealed the presence of multiple unidentified intralesional and intracellular coccoid microorganisms in the pathological gastroduodenal mucosa. Microaerophilic and anaerobic Gram-negative coccoid and filamentobacillary bacteria were cultured from the gastric aspirate. The triple therapy containing tetracycline for 14 days followed by 4 months treatment with omeprazole resulted in the resolution of the gastroduodenal pathology. The question, therefore, was raised regarding a possible role for the cultured coccoid bacteria in the pathogenesis. 16S rRNA gene sequence analysis of the isolated Gram-negative coccoid bacteria revealed a close relationship with Haemophilus haemolyticus. The unidentified coccoid microorganisms and cultured X and V factors independent coccoid bacteria, however, shared similar phenotypic, microbiological and pathological characteristics to the novel Gram-negative Streptococcaceae: Okadaella gastrococcus. BACKGROUND

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