A Modified Agar Plate Method for Detection of Strongyloides Stercoralis

Koga, Kaori; Kasuya, Shiro; Khamboonruang, Chirasak; Sukhavat, K; Ieda, Masatoshi; Takatsuka, Naoyoshi; Kita, Kiyoshi; Ohtomo, Hiroshi

doi:10.4269/ajtmh.1991.45.518

Cited by 227 publications

(194 citation statements)

References 0 publications

Supporting

Mentioning

176

Contrasting

Unclassified

Order By: Relevance

“…Even higher rates were observed in rural Laos (Sayasone et al, 2011(Sayasone et al, , 2009 (Baermann, 1917;Koga et al, 1991) and for E. vermicularis, the scotch-tape anal swab technique (Cho and Kang, 1975). Hence, diversity of the helminthic infection is underestimated in our study.…”

Section: Discussionmentioning

confidence: 54%

High prevalence of large trematode eggs in schoolchildren in Cambodia

et al. 2015

View full text Add to dashboard Cite

Large trematode eggs (LTE) resembling Fasciola spp. eggs were reportedly found in the stools of schoolchildren in Kandal province, Cambodia. This study aimed to assess the prevalence of LTE in the stools of children attending the affected school, identify potential risk factors for infection and ascertain the trematode species. We performed a cross-sectional study involving an in-depth questionnaire administered to schoolchildren at the affected school, and examined cattle droppings in the surrounding area and the livers of slaughtered cattle. Three stool samples were examined per child, using KatoKatz and formalin-ether concentration techniques. In addition, blood serum ELISA and coprological PCR was conducted for species clarification. Cattle droppings were examined by cup sedimentation and coprological ELISA. LTE were observed in the stools of 228 schoolchildren (46.5%). Two blood serum samples from schoolchildren were positive for Fasciola hepatica in a first ELISA but were negative in a confirmation immunofluorescence antibody test. Out of 221 PCR samples, only one tested positive for transmission from cattle to human is possible and public health services need to be alerted accordingly.3

show abstract

Section: Discussionmentioning

confidence: 54%

High prevalence of large trematode eggs in schoolchildren in Cambodia

et al. 2015

View full text Add to dashboard Cite

show abstract

“…The stool samples were transported to the laboratory within 2 hours after collection and subsequently analyzed by Baermann (Baermann, 1917), Koga Agar (Koga et al, 1991) and Kato-Katz (Katz et al, 1972) …”

Section: Sample Collectionmentioning

confidence: 99%

Evaluation of real-time PCR for Strongyloides stercoralis and hookworm as diagnostic tool in asymptomatic schoolchildren in Cambodia

et al. 2013

View full text Add to dashboard Cite

Diagnosis of soil-transmitted helminths such as Strongyloides stercoralis and hookworms (Ancylostoma duodenale and Necator americanus) is challenging due to irregular larval and egg output in infected individuals and insensitive conventional diagnostic procedures. Sensitive novel real-time PCR assays have been developed. Our study aimed to evaluate the real-time PCR assays as a diagnostic tool for detection of Strongyloides spp. and hookworms in a random stool sample of 218 asymptomatic schoolchildren in Cambodia.Overall prevalence of 17.4% (38/218) and 34.9% (76/218) were determined by real-time PCR for S. stercoralis and hookworms, respectively. Sensitivity and specificity of S. stercoralis specific real-time PCR as compared to the combination of Baermann/Koga Agar as gold standard were 88.9% and 92.7%, respectively. For hookworm specific realtime PCR a sensitivity of 78.9% and specificity of 78.9% were calculated. Co-infections were detectable by PCR in 12.8% (28/218) of individuals.Strongyloides stercoralis real-time PCR applied in asymptomatic cases showed a lower sensitivity compared to studies undertaken with symptomatic patients with the same molecular tool, yet it proved to be a valid supplement in the diagnosis of STH infection in Cambodia.

show abstract

“…Definitive diagnosis of strongyloidiasis is usually made through detection of the larvae in stool samples, which have low sensitivity 6 . Agar Plate culture is considered more efficient than other conventional methods in the parasitological diagnosis of S. stercoralis with high sensitivity 1,3 . However, it has not been used to screen candidates for transplantation.…”

mentioning

confidence: 99%