A modification of a Sandison‐Clark chamber for observation of transparent tissue in the rabbit's ear

Ebert, Robert H.; Florey, H. W.; Pullinger, B. D.

doi:10.1002/path.1700480110

Cited by 31 publications

(11 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…-The microscopic appearance of these cells conformed to descriptions previously made using the rabbit ear chamber technique Ebert et al, 1939;Ebert et al, 1940].…”

Section: Behaviour Of Macrophagessupporting

confidence: 81%

“…-The ingrowth of new blood vessels during wound healing involved the basic processes of sprouting, anastomosis and remodelling as described previously [Sandison, 1928;Clark, 1936;Ebert et al, 1939;Williams, 1954;Van Den Brenk, 1956;Cliff, 1963 (fig. 4) where the two sets of circles show the displacements of the cells from their positions at the end of cell division.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Kinetics of Wound Healing in Rabbit Ear Chambers, a Time Lapse Cinemicroscopic Study

Cliff

1965

Exp Physiol

View full text Add to dashboard Cite

Wound healing in rabbit ear chambers was studied by time lapse cinemicroscopy.Endothelial cells for the growth of blood vessel sprouts arose in a zone proximal to the growing fringe both by the mitotic division of existing endothelium and by the conservation for re-use of the endothelium of resorbing blood vessels. The migration of endothelial cells distally along the luminal surfaces of the walls of circulating blood vessels, into the proximal ends of developing blood vessel sprouts, and along the mid part and finally into the tips of blood vessel sprouts was demonstrated. New lumen of sprouts was produced by the formation, enlargement and coalescence of loculated spaces between apposed layers of endothelium. This process was considered to reflect secretory activity of the endothelial cells. Anastomosis between sprouts involved similar lumen formation and the spatial rearrangement of endothelial cells.Evidence supporting the conversion of fibroblasts into smooth muscle cells was obtained. Growth of lymphatic sprouts was also studied. The virtual absence of locomotion by macrophages during wound healing was confirmed and a possible explanation proposed. Mathematical analysis of the data obtained from film analysis revealed that the curves for blood vessel endothelial migration and sprout growth and lymphatic sprout growth were all derivable from the same general pseudo generating mathematical function.

show abstract

“…-The microscopic appearance of these cells conformed to descriptions previously made using the rabbit ear chamber technique Ebert et al, 1939;Ebert et al, 1940].…”

Section: Behaviour Of Macrophagessupporting

confidence: 81%

mentioning

confidence: 99%

Kinetics of Wound Healing in Rabbit Ear Chambers, a Time Lapse Cinemicroscopic Study

Cliff

1965

Exp Physiol

View full text Add to dashboard Cite

show abstract

“…3 B). This may bear some relation to the findings of Ebert, Florey, and Pullinger (1939), who prevented capillary invasion of the plasma clot in a rabbit-ear observation chamber by reducing the internal space with a central plug. This may explain why some suture-line abscesses are associated with large knots and the heavier grades of suture material, especially where frank infection is not a major factor.…”

Section: Discussionsupporting

confidence: 62%

The microvascular responses induced by materials used in operative surgery

Sewell¹

1966

Journal of British Surgery

View full text Add to dashboard Cite

“…Host Chamber Membrane.--Blood vessels and connective tissue formed in the freshly inserted ear chamber in the manner previously described (1,4,(12)(13)(14)(15)(16).…”

Section: Resultsmentioning

confidence: 99%

Observations on Tissue Grafts Established in Rabbit Ear Chambers

Hobbs

Cliff

1971

The Journal of Experimental Medicine

View full text Add to dashboard Cite

Early experiments using in vivo microscopy on mammalian tissues were reviewed by Clark et al. (1). The introduction of the transparent rabbit ear chamber, first described by Sandison (2, 3) has allowed microscopic observations to be made of the structure and function of a thin layer of living tissue in the mammal for periods up to several years (4).A modification of the Sandison-Clark rabbit ear chamber which allowed the mica cover slip to be removed, exposing the surface of the stable vascular membrane and then replaced, was adapted by Williams (4) to study graft tissue fragments implanted on the membrane. He found that autografts of a wide variety of tissues in fragments less than 1 mm diameter became vascularized by the anastomosis of graft vessels to the chamber vessels immediately beneath, and slowly became incorporated into the membrane. These grafts retained their structure and visible function (5). On the other hand, allograft tissues, with the exceptions of choroid plexus, ciliary body, V2 carcinoma, and to some extent adrenal, did not survive (5).Recently Greenblatt and others (6) established renal allografts in the hamster cheek pouch chamber and described the glomerular blood flow occurring during their brief survival. However, only fetal graft tissues could be used successfully and the glomerular blood flow always followed a preferential path in a relatively direct stream from the afferent to the efferent arterioles. Subsequently he has described fetal and neonatal heart grafts functioning in these chambers (7).With the eventual aim of studying the process of allograft rejection in vivo, we implanted tissues into the rabbit ear chamber b y a method adapted by Cliff et al. (8). Tissues successfully grafted include liver, kidney, myometrium, and thyroid. Materials and MethodsAnimal.--Rabbits used as recipients were obtained from a closed colony of half-lop rabbits maintained at the Australian National University. They were hardy animals with durable half-lop ears 16 cm in length, very suitable for transparent chamber insertion. Donor rabbits were either of a similar strain, though unrelated, or of an albino strain. Animals were fed on * Present address:

show abstract

A modification of a Sandison‐Clark chamber for observation of transparent tissue in the rabbit's ear

Cited by 31 publications

References 10 publications

Kinetics of Wound Healing in Rabbit Ear Chambers, a Time Lapse Cinemicroscopic Study

Kinetics of Wound Healing in Rabbit Ear Chambers, a Time Lapse Cinemicroscopic Study

The microvascular responses induced by materials used in operative surgery

Observations on Tissue Grafts Established in Rabbit Ear Chambers

Contact Info

Product

Resources

About